ID A0A2P6TQL0_CHLSO Unreviewed; 1199 AA.
AC A0A2P6TQL0;
DT 23-MAY-2018, integrated into UniProtKB/TrEMBL.
DT 23-MAY-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=Glutamine synthetase {ECO:0000256|RuleBase:RU004356};
DE EC=6.3.1.2 {ECO:0000256|RuleBase:RU004356};
GN ORFNames=C2E21_5011 {ECO:0000313|EMBL:PRW56325.1};
OS Chlorella sorokiniana (Freshwater green alga).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC Chlorellales; Chlorellaceae; Chlorella clade; Chlorella.
OX NCBI_TaxID=3076 {ECO:0000313|EMBL:PRW56325.1, ECO:0000313|Proteomes:UP000239899};
RN [1] {ECO:0000313|EMBL:PRW56325.1, ECO:0000313|Proteomes:UP000239899}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UTEX 1602 {ECO:0000313|Proteomes:UP000239899};
RX PubMed=29178410; DOI=10.1111/tpj.13789;
RA Arriola M.B., Velmurugan N., Zhang Y., Plunkett M.H., Hondzo H.,
RA Barney B.M.;
RT "Genome sequences of Chlorella sorokiniana UTEX 1602 and Micractinium
RT conductrix SAG 241.80: implications to maltose excretion by a green alga.";
RL Plant J. 93:566-586(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine +
CC phosphate; Xref=Rhea:RHEA:16169, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.1.2;
CC Evidence={ECO:0000256|RuleBase:RU004356};
CC -!- SIMILARITY: Belongs to the glutamine synthetase family.
CC {ECO:0000256|ARBA:ARBA00009897, ECO:0000256|PROSITE-ProRule:PRU01330,
CC ECO:0000256|RuleBase:RU000384}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PRW56325.1}.
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DR EMBL; LHPG02000009; PRW56325.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2P6TQL0; -.
DR STRING; 3076.A0A2P6TQL0; -.
DR OrthoDB; 168438at2759; -.
DR Proteomes; UP000239899; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0004356; F:glutamine synthetase activity; IEA:UniProtKB-EC.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0006542; P:glutamine biosynthetic process; IEA:InterPro.
DR GO; GO:0043170; P:macromolecule metabolic process; IEA:UniProt.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProt.
DR CDD; cd19519; RecA-like_CDC48_r1-like; 1.
DR CDD; cd19528; RecA-like_CDC48_r2-like; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.10.330.10; -; 1.
DR Gene3D; 6.10.20.150; -; 1.
DR Gene3D; 3.10.20.70; Glutamine synthetase, N-terminal domain; 1.
DR Gene3D; 3.30.590.10; Glutamine synthetase/guanido kinase, catalytic domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR005938; AAA_ATPase_CDC48.
DR InterPro; IPR041569; AAA_lid_3.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR003960; ATPase_AAA_CS.
DR InterPro; IPR004201; Cdc48_dom2.
DR InterPro; IPR029067; CDC48_domain_2-like_sf.
DR InterPro; IPR003338; CDC4_N-term_subdom.
DR InterPro; IPR008147; Gln_synt_N.
DR InterPro; IPR036651; Gln_synt_N_sf.
DR InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR InterPro; IPR008146; Gln_synth_cat_dom.
DR InterPro; IPR027303; Gln_synth_gly_rich_site.
DR InterPro; IPR027302; Gln_synth_N_conserv_site.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR01243; CDC48; 1.
DR PANTHER; PTHR23077; AAA-FAMILY ATPASE; 1.
DR PANTHER; PTHR23077:SF171; ATPASE FAMILY PROTEIN 2 HOMOLOG; 1.
DR Pfam; PF00004; AAA; 2.
DR Pfam; PF17862; AAA_lid_3; 2.
DR Pfam; PF02933; CDC48_2; 1.
DR Pfam; PF02359; CDC48_N; 1.
DR Pfam; PF00120; Gln-synt_C; 1.
DR SMART; SM00382; AAA; 2.
DR SMART; SM01072; CDC48_2; 1.
DR SMART; SM01073; CDC48_N; 1.
DR SMART; SM01230; Gln-synt_C; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF54585; Cdc48 domain 2-like; 1.
DR SUPFAM; SSF54368; Glutamine synthetase, N-terminal domain; 1.
DR SUPFAM; SSF55931; Glutamine synthetase/guanido kinase; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS00674; AAA; 2.
DR PROSITE; PS00180; GLNA_1; 1.
DR PROSITE; PS00181; GLNA_ATP; 1.
DR PROSITE; PS51986; GS_BETA_GRASP; 1.
DR PROSITE; PS51987; GS_CATALYTIC; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU004356};
KW Cell cycle {ECO:0000313|EMBL:PRW56325.1};
KW Cell division {ECO:0000313|EMBL:PRW56325.1};
KW Ligase {ECO:0000256|RuleBase:RU004356};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU004356};
KW Reference proteome {ECO:0000313|Proteomes:UP000239899}.
FT DOMAIN 42..122
FT /note="GS beta-grasp"
FT /evidence="ECO:0000259|PROSITE:PS51986"
FT DOMAIN 139..375
FT /note="GS catalytic"
FT /evidence="ECO:0000259|PROSITE:PS51987"
FT REGION 1157..1199
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1199 AA; 132655 MW; A6F290FA24A78191 CRC64;
MSPSGEPYVL PPCFGTSGRE EQLLDGTLWD ARFKDLSQHG RIAAEYIWIG GTGQDMRSKT
RVLDKVPSKP EELPMWNYDG SSTGQAPGDD SEVYLVPRAI YKDPFRGGDN ILVMCDTYEP
PRVLPDGTVS ALKPIPTNTR AACAEVMEKV KAEEPWFGIE QEYTLLNART KWPLGWPTNG
YPGPQGPYYC SAGAGAAIGR EIVESHMKCC MFAGVNISGV NAEVMPAQWE YQVGPCTGIA
MGDDLWMSRY IMYRLAELYN VEVTFDPKPI PGDWNGAGGH TNFSSKATRT AETGWQAIQD
QIAKLERRHA VHIAAYGEGN ERRLTGKHET SSIHDFSWGV ANRGCSIRVG RMVPVEKCGY
YEDRRPASNL DPYVVTRLIC ETPAGEGQPP AHGDHGKKDF STAILERKKS PNRLVVDDAV
NQDDNSVVTL HPKTMEVLEL FRGDTVLLKG KKRKDTVCIV LADDTVEEAK IRMNKVVRKN
LRVRLGDIVS VHQCPDVKYG KRIHVLPFED TIEGISGNLF DAFLKPYFNE AYRPVRKGDT
FLVRGGMRTV EFKVVETDPA EYCIVAPDTE IFCEGEPIRR EDEERLDEVG YDDVGGVRKQ
MAQIRELVEL PLRHPQLFKT IGVKPPKGIL LYGPPGSGKT LIARAVANET GAFFFLINGP
EIMSKLAGES ESNLRKAFEE AEKNSPAIIF IDEIDSIAPK REKTQGEVER RIVSQLLTLM
DGLKSRSHVI VMGATNRPNS IDPALRRFGR FDREIDIGVP DEIGRLEVLR IHTRNMKLDD
DVDLEAISRD THGYVGADLA ALCTEAALQC IREKMDVIDL EDESIDAEVL NSMAVTMDHF
KTALGLSNPS ALRETVVEVP NVTWDDIGGL ENVKRELQET VQYPVEHPEK FEKYGMAPSK
GVLFYGPPGC GKTLLAKAIA NECQANFISV KGPELLTMWF GESEANVREI FDKARQSAPC
VLFFDELDSI AVQRGSSSGD AGGAADRVLN QMLTEMDGMN AKKTVFIIGA TNRPDIIDPA
LMRPGRLDQL IYIPLPDEGS RRSIFKAALR KSPVAPNVDL DHLAKHTQGY SGADITEICQ
RAVKYAIRES IEKDIERNRR KQENPDLMDE DDTDPVPCIT VAHFEESMRF ARRSVSDADI
RKYQAFAQTL QQSRGFGSEF RFPDSGGAAA PAADGAAPAA AAGTFASAGA ADEDDDLYS
//
