ID A0A2P6TUS1_CHLSO Unreviewed; 826 AA.
AC A0A2P6TUS1;
DT 23-MAY-2018, integrated into UniProtKB/TrEMBL.
DT 23-MAY-2018, sequence version 1.
DT 24-JAN-2024, entry version 17.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
GN ORFNames=C2E21_3298 {ECO:0000313|EMBL:PRW57819.1};
OS Chlorella sorokiniana (Freshwater green alga).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC Chlorellales; Chlorellaceae; Chlorella clade; Chlorella.
OX NCBI_TaxID=3076 {ECO:0000313|EMBL:PRW57819.1, ECO:0000313|Proteomes:UP000239899};
RN [1] {ECO:0000313|EMBL:PRW57819.1, ECO:0000313|Proteomes:UP000239899}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UTEX 1602 {ECO:0000313|Proteomes:UP000239899};
RX PubMed=29178410; DOI=10.1111/tpj.13789;
RA Arriola M.B., Velmurugan N., Zhang Y., Plunkett M.H., Hondzo H.,
RA Barney B.M.;
RT "Genome sequences of Chlorella sorokiniana UTEX 1602 and Micractinium
RT conductrix SAG 241.80: implications to maltose excretion by a green alga.";
RL Plant J. 93:566-586(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|RuleBase:RU000675};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PRW57819.1}.
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DR EMBL; LHPG02000006; PRW57819.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2P6TUS1; -.
DR STRING; 3076.A0A2P6TUS1; -.
DR OrthoDB; 5489808at2759; -.
DR Proteomes; UP000239899; Unassembled WGS sequence.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0031072; F:heat shock protein binding; IEA:InterPro.
DR GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd10719; DnaJ_zf; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR048912; BetaGal1-like_ABD1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR001305; HSP_DnaJ_Cys-rich_dom.
DR PANTHER; PTHR23421:SF165; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21317; BetaGal_ABD_1; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW Reference proteome {ECO:0000313|Proteomes:UP000239899};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 21..826
FT /note="Beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5015126048"
FT DOMAIN 38..373
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 422..545
FT /note="Beta-galactosidase 1-like first all-beta"
FT /evidence="ECO:0000259|Pfam:PF21317"
FT DOMAIN 586..655
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
SQ SEQUENCE 826 AA; 90485 MW; D0A132C6974C73C7 CRC64;
MPRAIAPLLL LALLAGGAAA GAATAVGASP SFGIKDDQLL LDGKPIQVIS GSMHYFRIHP
DLWEDRLARA VAMGINTVEV YTPWNMHEPY PGQYVWSGIA DLERWMDLIQ KSGLKVLLRP
GPYICAEWDN GGFPHWFASS KVAGGRTMRM RTNDPLYLDH VDRWWAVLFS KLRRYLHENG
GPILMVQIEN EYGFCGEDKE YLRRLIDITR THLSSDVLLF STDPPGVASR GSIMGDELYT
VVDFGPGWFD PSSAFAAQAA MNAPGKSPPF CSEFYTGWLT HWGEAMANTS SEVLFKDTQV
LLEWANSTAS LSFYMIHGGT NFGFWAGANV DGERYLPHIT SYDYDAPISE AGDYCQPGIG
GDCKYYVLRD LIAKHTGRSL PEPPPRPAIR SYGKVDVKES LPLMEAVPAL FSGGGIRAEV
PDIMEEYGQR WGLILYRTRL LAKALEKDGA LDLGGAPHDY ATVFVDGELA GRLDRSEPAT
NLTLRARHAQ GKGKGNQIQL DILVEAMGRQ NFGCDTGNWD WKGLTSQNVT LNGKRLRGWE
VFPLQLDDVS DLAYASGQSG AALVSAARRR MLEVPAVGGK RNADAPAFFR GTFEVDAATA
VRGPSGHLAD TYLALHGWSK GIAWVNGFNL GWYWPRLGPQ MTLYVPGPVL REGANEVVLL
EVERAAHDEA VLLDDKPDFW GPAAWLRPGG PSAARRQALL RPRALGPRGA SGSFDEGGLD
YKLAKELETA TWDVRRQAVT KHLELLYQAS QSQSERNKVE QCECCNGSGE KECAWCHGTG
AMTVGDMLYC SEGGCSPCPV CRGTGACKCE HCRGTGRRAS WLPLAP
//
