ID A0A2P8KFL4_9BURK Unreviewed; 1166 AA.
AC A0A2P8KFL4;
DT 23-MAY-2018, integrated into UniProtKB/TrEMBL.
DT 23-MAY-2018, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=C7T35_25595 {ECO:0000313|EMBL:PSL81754.1};
OS Variovorax sp. WS11.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Variovorax.
OX NCBI_TaxID=1105204 {ECO:0000313|EMBL:PSL81754.1, ECO:0000313|Proteomes:UP000241151};
RN [1] {ECO:0000313|EMBL:PSL81754.1, ECO:0000313|Proteomes:UP000241151}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WS11 {ECO:0000313|EMBL:PSL81754.1,
RC ECO:0000313|Proteomes:UP000241151};
RA Crombie A.T., Mejia-Florez N.L., Murrell C.;
RT "Isoprene degraders from the Poplar phyllosphere.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PSL81754.1}.
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DR EMBL; PXZZ01000040; PSL81754.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2P8KFL4; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000241151; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd07433; PHP_PolIIIA_DnaE1; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR049821; PolIIIA_DnaE1_PHP.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 3..70
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1166 AA; 129338 MW; D047BF871BE66EE3 CRC64;
MFVHLRLHTE FSVVDGTNRI DEVVKAAAAD KQPALAITDL NNLFGGVKFY KEARGKGVKP
VLGAEVFVDG LGTEPGVLTR MLLLVQNFEG YLHLSELLAR AWTQNVGRGQ AQAVCKLAWI
EELQGGLIAL SGAQAGPLGA PLLQGQAERA AEVALQLAGL FPHRFYIELQ RAGRPEDEPH
VVAAVQLAAR LNLPVVATHP VQFATAEDFE AHEARVCISE GEILGNARRV RRFTPQQYFK
SAAEMAQLFA DVPSAIANTV EIAKRCNLTL TLGKPRLPDF PTPNGMPIEE YFRHASFEGL
EERLAHLYPD AAKRDAERPR YVERLEFEIN TILKMGFPGY FLIVGDFINW AKKNGCPVGP
GRGSGAGSLV AYALKITDLD PLEYKLLFER FLNPERVSMP DFDIDFCQGN RDRVIDYVKD
KYGRDAVSQI ATFGTMAARA AIRDVGRVLD MSYTFCDGIS KLIPNKPGMS VTLQYPPVPK
IEGDKNNYAI EMEPQLAARI EKEEEVRMLV ELAQKLEGMT RNIGMHAGGV LIAPGKLTDF
CPLYQQPGSD SAVSQYDKDD VEAIGLVKFD FLGLATLTIL EIAKDFIVQR HKGQEGFAYE
NIRLDDTPTY KLFAEGKTEA VFQFESRGMQ GMLKDARPTR LEDLIALNAL YRPGPMDLIP
SFVARKHGRE PVEYPHPLVE EMLSETYGIM VYQEQVMQTA QILGGYSLGG ADLLRRAMGK
KKAEEMAEHR LIFRAGAAKN GISEEKADEI FDLMEKFAGY GFNKSHAAAY SLLAYHTGWL
KVHYTAEFFC ANMTVEMDDT DKLKVLFEDA QKNFGMTFEP PDVNRGNYRF EPVTDKVIRY
GLGAVKGTGQ LAVEAVVRAR EEGGPFMSLF DFCVRVDRQR INKRTVEALI KAGAFDALQQ
NRAALVASID RAFEFASATE ANASQVDIFG DCEHGSATQE PELVDATPWG VKERLTLEKT
AIGFYLSGHL FDEVAHEVRR FCKREIDDLI DSREQQVIAG IVSDMRVING QRGRLAIFKL
DDKSASIEAT ADEALINNNR NTLKDDELVI VSGRLQPGRG GFEARFQVQQ VWDLATARCR
FGKFLRVAVN GKAPDIARLV REFPPRTEQS EHGELLQGLA VRLSMARGGA QVELQLGERA
KFFPTDAALA SWMAQAEAGK ASVVYE
//
