ID A0A2P8KLH0_9BURK Unreviewed; 564 AA.
AC A0A2P8KLH0;
DT 23-MAY-2018, integrated into UniProtKB/TrEMBL.
DT 23-MAY-2018, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Long-chain-fatty-acid--CoA ligase {ECO:0000256|ARBA:ARBA00039545};
DE EC=6.2.1.3 {ECO:0000256|ARBA:ARBA00026121};
DE AltName: Full=Long-chain acyl-CoA synthetase {ECO:0000256|ARBA:ARBA00042773};
GN ORFNames=C7T35_14260 {ECO:0000313|EMBL:PSL83817.1}, GXN78_00220
GN {ECO:0000313|EMBL:NDZ11309.1};
OS Variovorax sp. WS11.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Variovorax.
OX NCBI_TaxID=1105204 {ECO:0000313|EMBL:PSL83817.1, ECO:0000313|Proteomes:UP000241151};
RN [1] {ECO:0000313|EMBL:PSL83817.1, ECO:0000313|Proteomes:UP000241151}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WS11 {ECO:0000313|EMBL:PSL83817.1,
RC ECO:0000313|Proteomes:UP000241151};
RA Crombie A.T., Mejia-Florez N.L., Murrell C.;
RT "Isoprene degraders from the Poplar phyllosphere.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:NDZ11309.1, ECO:0000313|Proteomes:UP000479786}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WS11 {ECO:0000313|EMBL:NDZ11309.1,
RC ECO:0000313|Proteomes:UP000479786};
RA Dawson R.A., Larke-Mejia N.L., Crombie A.T., Ul-Haque M.F., Murrell J.C.;
RT "Isoprene oxidation by the Gram negative model bacterium Variovorax sp.
RT WS11.";
RL Submitted (FEB-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC {ECO:0000256|ARBA:ARBA00005005}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004170};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004170}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PSL83817.1}.
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DR EMBL; JAAGOW010000001; NDZ11309.1; -; Genomic_DNA.
DR EMBL; PXZZ01000017; PSL83817.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2P8KLH0; -.
DR OrthoDB; 9787658at2; -.
DR Proteomes; UP000241151; Unassembled WGS sequence.
DR Proteomes; UP000479786; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.10.129.10; Hotdog Thioesterase; 1.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR029069; HotDog_dom_sf.
DR PANTHER; PTHR43767; LONG-CHAIN-FATTY-ACID--COA LIGASE; 1.
DR PANTHER; PTHR43767:SF8; LONG-CHAIN-FATTY-ACID--COA LIGASE; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF13193; AMP-binding_C; 1.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR SUPFAM; SSF54637; Thioesterase/thiol ester dehydrase-isomerase; 1.
PE 4: Predicted;
KW Ligase {ECO:0000313|EMBL:PSL83817.1}.
FT DOMAIN 127..301
FT /note="AMP-dependent synthetase/ligase"
FT /evidence="ECO:0000259|Pfam:PF00501"
FT DOMAIN 372..440
FT /note="AMP-binding enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13193"
SQ SEQUENCE 564 AA; 59309 MW; 096F5320E1282F36 CRC64;
MSPELLPLLA DRDPDAPLAW RAGVPVSARA FLADVARLAP QLPDGGPVVN LCTDRYAFAL
GLAAALVRGQ TSLLPPDARP DTLARLVEPG SRTYAVLDDT GIETPGMPRV QIEHRLPPAG
AAPQAVPAIA GSLHAVSLLT SGSTGTPQPH AKSWKTLVGD VAAAVGRLSE LLGRRSLAGL
TLVATVPVQH SYGLESSALL AMLGGAAFDS GRPFFPADVA QALASVPQPR ALVTTPFHLK
TLLLSGIALP PVDLILSATA PLSPQLAAQA EQAMGGQMIE IYGSTESGQV ATRRPTRSEV
WETFGEIRVH AEHDAEGAER FVFAGDFVPE PTPMADVLEL VDDRRFRLLG RANDLIHVAG
RRSSLGYLNH HLNSIPAVED GAFWLPDDVT DGVVRPVAFV VAPALDAHAI IAALRERLEA
VFVPRRVVHV HAFPREGTGK LTARALREFA LAQLASDGTP VQLTHEVPVD HPAFAGHFPG
QPLLPGALLL AEVLEAAQGV PALAARLGPR PTLAAAKFLA PVRPGSTLRI DLNPESGASR
GLRFEVRVAE VVAASGRWTP GSTP
//
