ID A0A2P8KU65_9BURK Unreviewed; 833 AA.
AC A0A2P8KU65;
DT 23-MAY-2018, integrated into UniProtKB/TrEMBL.
DT 23-MAY-2018, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=K(+)-insensitive pyrophosphate-energized proton pump {ECO:0000256|HAMAP-Rule:MF_01129};
DE EC=7.1.3.1 {ECO:0000256|HAMAP-Rule:MF_01129};
DE AltName: Full=Membrane-bound proton-translocating pyrophosphatase {ECO:0000256|HAMAP-Rule:MF_01129};
DE AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase {ECO:0000256|HAMAP-Rule:MF_01129};
DE Short=H(+)-PPase {ECO:0000256|HAMAP-Rule:MF_01129};
GN Name=hppA {ECO:0000256|HAMAP-Rule:MF_01129,
GN ECO:0000313|EMBL:PSL86490.1};
GN ORFNames=C7T35_00490 {ECO:0000313|EMBL:PSL86490.1}, GXN78_02100
GN {ECO:0000313|EMBL:NDZ11676.1};
OS Variovorax sp. WS11.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Variovorax.
OX NCBI_TaxID=1105204 {ECO:0000313|EMBL:PSL86490.1, ECO:0000313|Proteomes:UP000241151};
RN [1] {ECO:0000313|EMBL:PSL86490.1, ECO:0000313|Proteomes:UP000241151}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WS11 {ECO:0000313|EMBL:PSL86490.1,
RC ECO:0000313|Proteomes:UP000241151};
RA Crombie A.T., Mejia-Florez N.L., Murrell C.;
RT "Isoprene degraders from the Poplar phyllosphere.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:NDZ11676.1, ECO:0000313|Proteomes:UP000479786}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WS11 {ECO:0000313|EMBL:NDZ11676.1,
RC ECO:0000313|Proteomes:UP000479786};
RA Dawson R.A., Larke-Mejia N.L., Crombie A.T., Ul-Haque M.F., Murrell J.C.;
RT "Isoprene oxidation by the Gram negative model bacterium Variovorax sp.
RT WS11.";
RL Submitted (FEB-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Proton pump that utilizes the energy of pyrophosphate
CC hydrolysis as the driving force for proton movement across the
CC membrane. Generates a proton motive force. {ECO:0000256|HAMAP-
CC Rule:MF_01129}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=diphosphate + H(+)(in) + H2O = 2 H(+)(out) + 2 phosphate;
CC Xref=Rhea:RHEA:13973, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:43474; EC=7.1.3.1;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01129};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01129};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01129}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|HAMAP-Rule:MF_01129};
CC Multi-pass membrane protein {ECO:0000256|HAMAP-Rule:MF_01129}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the H(+)-translocating pyrophosphatase (TC
CC 3.A.10) family. K(+)-insensitive subfamily. {ECO:0000256|HAMAP-
CC Rule:MF_01129}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_01129}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PSL86490.1}.
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DR EMBL; JAAGOW010000001; NDZ11676.1; -; Genomic_DNA.
DR EMBL; PXZZ01000001; PSL86490.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2P8KU65; -.
DR OrthoDB; 9808652at2; -.
DR Proteomes; UP000241151; Unassembled WGS sequence.
DR Proteomes; UP000479786; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009678; F:diphosphate hydrolysis-driven proton transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0004427; F:inorganic diphosphate phosphatase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR CDD; cd07185; OmpA_C-like; 1.
DR Gene3D; 3.30.1330.60; OmpA-like domain; 1.
DR HAMAP; MF_01129; PPase_energized_pump; 1.
DR InterPro; IPR006665; OmpA-like.
DR InterPro; IPR036737; OmpA-like_sf.
DR InterPro; IPR004131; PPase-energised_H-pump.
DR NCBIfam; TIGR01104; V_PPase; 1.
DR PANTHER; PTHR31998; K(+)-INSENSITIVE PYROPHOSPHATE-ENERGIZED PROTON PUMP; 1.
DR PANTHER; PTHR31998:SF55; K(+)-STIMULATED PYROPHOSPHATE-ENERGIZED SODIUM PUMP; 1.
DR Pfam; PF03030; H_PPase; 1.
DR Pfam; PF00691; OmpA; 1.
DR PIRSF; PIRSF001265; H+-PPase; 1.
DR SUPFAM; SSF103088; OmpA-like; 1.
DR PROSITE; PS51123; OMPA_2; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|HAMAP-Rule:MF_01129};
KW Hydrogen ion transport {ECO:0000256|HAMAP-Rule:MF_01129};
KW Hydrolase {ECO:0000313|EMBL:PSL86490.1};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065, ECO:0000256|HAMAP-
KW Rule:MF_01129};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_01129};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|HAMAP-Rule:MF_01129};
KW Translocase {ECO:0000256|ARBA:ARBA00022967, ECO:0000256|HAMAP-
KW Rule:MF_01129};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|HAMAP-
KW Rule:MF_01129};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, ECO:0000256|HAMAP-
KW Rule:MF_01129};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|HAMAP-Rule:MF_01129}.
FT TRANSMEM 6..28
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01129"
FT TRANSMEM 57..75
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01129"
FT TRANSMEM 81..102
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01129"
FT TRANSMEM 122..149
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01129"
FT TRANSMEM 161..182
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01129"
FT TRANSMEM 240..258
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01129"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01129"
FT TRANSMEM 296..320
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01129"
FT TRANSMEM 332..351
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01129"
FT TRANSMEM 389..406
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01129"
FT TRANSMEM 412..432
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01129"
FT TRANSMEM 508..528
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01129"
FT TRANSMEM 576..595
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01129"
FT TRANSMEM 602..624
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01129"
FT TRANSMEM 664..684
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01129"
FT DOMAIN 727..833
FT /note="OmpA-like"
FT /evidence="ECO:0000259|PROSITE:PS51123"
FT REGION 697..724
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 812..833
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 702..716
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 467
FT /note="Determinant of potassium independence"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01129"
SQ SEQUENCE 833 AA; 84607 MW; 55BF43BF537E636A CRC64;
MIGNTPLVLA IVCGLVAVGY GFWARSWILS KDPGNARMQE IAAAIQTGAA AYLAKQYRTI
AIVGLVLAIL IGVFLDVTTA VGFVIGAVLS GACGFIGMNV SVRANVRTAQ AATQGIGPAL
DVAFRGGAIT GMLVVGLGLL GVTVFFWFLA GNGQLTPNTK LATLLNPLIG FAFGSSLISI
FARLGGGIFT KGADVGADLV GKVEAGIPED DPRNPAVIAD NVGDNVGDCA GMAADLFETY
AVTLIATMVL GALMVTAAPV NAVLYPLALG AVSIIASIIG CFFVRASPGM VNVMPALYKG
LAVAGILSLI AFWFVTQWLI PDNAIAASGS QLKLFGACFV GLALTAALVW ITEFYTGTQY
SPVRHIAQAS TTGHGTNIIA GLGVSMRSTA WPVLCVCIAI MASYWLGGLY GIAVAATAML
SMAGIVVALD AYGPITDNAG GIAEMSELPA SVRDITDPLD AVGNTTKAVT KGYAIGSAGL
ASLVLFADYT HKLETYGQAI SFNLSEPMVI VGLFIGGLIP YLFGAMAMEA VGRAAGSVVE
EVRRQFREIP GIMEGTGKPE YGRAVSMLTG AAIKEMMIPS LLPVVVPILV GLLLGPKALG
GLLMGTIVTG LFVAISMCTG GGAWDNAKKY IEDGHHGGKG SEAHKAAVTG DTVGDPYKDT
AGPAVNPLIK IINIVALLIV PLVVKFHSGD AGAMTAPAAS PAPAVAPAPA PAPAPAAAPA
AAGDTGASIK TDGGVVKFYF ASASAEVPAG SSAALADIVK SVQSGGRVLV SGYHDATGDA
EKNAELAKQR AMAVGAALRM AGVPDDKVEL AKPAQAQADG PPAEARRVEV KLQ
//
