ID A0A2R5HG32_9LACT Unreviewed; 383 AA.
AC A0A2R5HG32;
DT 20-JUN-2018, integrated into UniProtKB/TrEMBL.
DT 20-JUN-2018, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE SubName: Full=Hydroxymethylglutaryl-CoA synthase {ECO:0000313|EMBL:GBG96285.1};
GN Name=pksG {ECO:0000313|EMBL:GBG96285.1};
GN ORFNames=NtB2_00396 {ECO:0000313|EMBL:GBG96285.1};
OS Lactococcus termiticola.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus.
OX NCBI_TaxID=2169526 {ECO:0000313|EMBL:GBG96285.1, ECO:0000313|Proteomes:UP000245021};
RN [1] {ECO:0000313|EMBL:GBG96285.1, ECO:0000313|Proteomes:UP000245021}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NtB2 {ECO:0000313|EMBL:GBG96285.1,
RC ECO:0000313|Proteomes:UP000245021};
RA Noda S., Aihara C., Yuki M., Ohkuma M.;
RT "Draft Genome Sequence of Lactococcus sp. Strain NtB2 (JCM 32569), Isolated
RT from the Gut of the Higher Termite Nasutitermes takasagoensis.";
RL Genome Announc. 6:e00445-18(2018).
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. HMG-CoA synthase
CC family. {ECO:0000256|ARBA:ARBA00007061}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GBG96285.1}.
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DR EMBL; BFFO01000002; GBG96285.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2R5HG32; -.
DR Proteomes; UP000245021; Unassembled WGS sequence.
DR GO; GO:0004421; F:hydroxymethylglutaryl-CoA synthase activity; IEA:InterPro.
DR GO; GO:0006084; P:acetyl-CoA metabolic process; IEA:InterPro.
DR GO; GO:0010142; P:farnesyl diphosphate biosynthetic process, mevalonate pathway; IEA:InterPro.
DR CDD; cd00827; init_cond_enzymes; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR013746; HMG_CoA_synt_C_dom.
DR InterPro; IPR013528; HMG_CoA_synth_N.
DR InterPro; IPR011554; HMG_CoA_synthase_prok.
DR InterPro; IPR016039; Thiolase-like.
DR NCBIfam; TIGR01835; HMG-CoA-S_prok; 1.
DR PANTHER; PTHR43323; 3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE; 1.
DR PANTHER; PTHR43323:SF2; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR Pfam; PF08540; HMG_CoA_synt_C; 2.
DR Pfam; PF01154; HMG_CoA_synt_N; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000245021}.
FT DOMAIN 4..167
FT /note="Hydroxymethylglutaryl-coenzyme A synthase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01154"
FT DOMAIN 181..246
FT /note="Hydroxymethylglutaryl-coenzyme A synthase C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08540"
FT DOMAIN 257..364
FT /note="Hydroxymethylglutaryl-coenzyme A synthase C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08540"
FT ACT_SITE 81
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-1"
FT ACT_SITE 113
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-1"
FT ACT_SITE 235
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-1"
FT BINDING 145
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT BINDING 150
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT BINDING 244
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT BINDING 275
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
SQ SEQUENCE 383 AA; 42151 MW; DC7CC9C57EC7CC05 CRC64;
MMNTVGIDKM SFFVPPYFLE MDELASARQV EPAKLHIGIG QDQMAVSPAS QDIVTLAANA
AADILTEADK AAIDMVIVGT ESSIDESKAS AVIVHGLLDI QPFARSIEIK EACYGATAGL
QLAKNHVQQH PDRKVLVIAS DIAKYGLASG GEPTQGAGAV AMLVSANPSL LALHNDEVSL
SQDIYDFWRP TGQTYPSVDG KFSNETYINA FARVWEEYKT RTNLDFQDFS ALAFHTPYTK
MGKKALLPVL EGHADQERLM SRYEEGIVYN RRVGNLYTGS LYLSLLSLLE NSRALSAGDR
LGLFSYGSGT VAEFYSAELM PGFEQALLTE KHQAMLENRE ALDISAYEAM FSETLDLEQE
KSFQDQTPFS VCEIKDGIRH YKK
//
