ID A0A2R5HJL8_9LACT Unreviewed; 760 AA.
AC A0A2R5HJL8;
DT 20-JUN-2018, integrated into UniProtKB/TrEMBL.
DT 20-JUN-2018, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=peptidoglycan glycosyltransferase {ECO:0000256|ARBA:ARBA00012555};
DE EC=2.4.1.129 {ECO:0000256|ARBA:ARBA00012555};
GN ORFNames=NtB2_00603 {ECO:0000313|EMBL:GBG96491.1};
OS Lactococcus termiticola.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus.
OX NCBI_TaxID=2169526 {ECO:0000313|EMBL:GBG96491.1, ECO:0000313|Proteomes:UP000245021};
RN [1] {ECO:0000313|EMBL:GBG96491.1, ECO:0000313|Proteomes:UP000245021}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NtB2 {ECO:0000313|EMBL:GBG96491.1,
RC ECO:0000313|Proteomes:UP000245021};
RA Noda S., Aihara C., Yuki M., Ohkuma M.;
RT "Draft Genome Sequence of Lactococcus sp. Strain NtB2 (JCM 32569), Isolated
RT from the Gut of the Higher Termite Nasutitermes takasagoensis.";
RL Genome Announc. 6:e00445-18(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-
CC Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-
CC (1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-
CC cis-undecaprenyl diphosphate = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-
CC D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl
CC diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+);
CC Xref=Rhea:RHEA:23708, Rhea:RHEA-COMP:9602, Rhea:RHEA-COMP:9603,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58405, ChEBI:CHEBI:60033,
CC ChEBI:CHEBI:78435; EC=2.4.1.129;
CC Evidence={ECO:0000256|ARBA:ARBA00023988};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GBG96491.1}.
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DR EMBL; BFFO01000003; GBG96491.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2R5HJL8; -.
DR Proteomes; UP000245021; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR GO; GO:0008955; F:peptidoglycan glycosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009002; F:serine-type D-Ala-D-Ala carboxypeptidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 6.20.370.110; -; 1.
DR Gene3D; 1.10.3810.10; Biosynthetic peptidoglycan transglycosylase-like; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR001264; Glyco_trans_51.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR InterPro; IPR036950; PBP_transglycosylase.
DR InterPro; IPR001460; PCN-bd_Tpept.
DR NCBIfam; TIGR02074; PBP_1a_fam; 1.
DR PANTHER; PTHR32282; BINDING PROTEIN TRANSPEPTIDASE, PUTATIVE-RELATED; 1.
DR PANTHER; PTHR32282:SF32; PENICILLIN-BINDING PROTEIN 2A; 1.
DR Pfam; PF00912; Transgly; 1.
DR Pfam; PF00905; Transpeptidase; 1.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR SUPFAM; SSF53955; Lysozyme-like; 1.
PE 4: Predicted;
KW Carboxypeptidase {ECO:0000256|ARBA:ARBA00022645};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000245021};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 103..273
FT /note="Glycosyl transferase family 51"
FT /evidence="ECO:0000259|Pfam:PF00912"
FT DOMAIN 374..642
FT /note="Penicillin-binding protein transpeptidase"
FT /evidence="ECO:0000259|Pfam:PF00905"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 442..462
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 706..738
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 706..730
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 760 AA; 82901 MW; B9C7F24B3A43CD28 CRC64;
MTEEQERLTR RRKKATGDQS KTAKKETIIN KESNPVKYYA SKVWRPFRRF WKKYSLTKVS
LIALIVLVVA TGSYLFYLAK SANVSVLQSS ISAQTEIIDK NGNVAGELYG SKGSSVEFNQ
ISDNVKNAVV ATEDRTFYKN HGVNLGRFIL ATVTLGRFGG GSTITQQLAK NAYLTQAQTI
DRKAREFFLA LEINKHYSKQ QILTMYLNNS YFGNGVWGIQ DASEKYFGIP ASDLTVDEAA
TLAGMLKGPE IYNPLYKNAQ YAVPRRNTVL QNMVNAGFLK QDEADGYAKV DLLSELKDNY
VPQTKSYKYP SYFNAVITEA ENKFGMTLQE IMNNGYKIYT SLDSNMQTGM QATYSNVSLF
PQAADGTYAQ SGSVAIDPKT GGVSALVGNV DTGDYNAFLN FNYATQSKRS TGSTIKPLIA
YLPAVQSGWS TDKVLSDVGK NFGSSSNPYE PKDDDGMTGH TGQYDGTNYA SGTLPMYQAL
ANSYNIPALN TYQSIGPEKG NELGKTFGLN LTDKNNILPT VLGSGVETNP WQMAQAYATF
ANGGVMNTAH IINKVENAAG QTIHKAKVSQ TRVMSESDAD KMTSMMQGTF TNGSAWNAAP
SSYAMAGKTG TNNTTDQWVI GYTPDIAIAL WVGFPQESNA QYQLEGTSSG QTSVIFRDEA
SYILPYTPGT KFNSENAYAA HGISPLAADW TQTRQNQDNI VATEQYKNNT TGNAPVQSSG
SNSSDSSSND SKLGEKIKKG LNDVGNAAKD LWDKASNIFN
//
