ID A0A2R6R7T6_ACTCC Unreviewed; 533 AA.
AC A0A2R6R7T6;
DT 20-JUN-2018, integrated into UniProtKB/TrEMBL.
DT 20-JUN-2018, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=Pyruvate kinase {ECO:0000256|ARBA:ARBA00012142, ECO:0000256|RuleBase:RU000504};
DE EC=2.7.1.40 {ECO:0000256|ARBA:ARBA00012142, ECO:0000256|RuleBase:RU000504};
GN ORFNames=CEY00_Acc08442 {ECO:0000313|EMBL:PSS23618.1};
OS Actinidia chinensis var. chinensis (Chinese soft-hair kiwi).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; Ericales; Actinidiaceae; Actinidia.
OX NCBI_TaxID=1590841 {ECO:0000313|EMBL:PSS23618.1, ECO:0000313|Proteomes:UP000241394};
RN [1] {ECO:0000313|EMBL:PSS23618.1, ECO:0000313|Proteomes:UP000241394}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Red5 {ECO:0000313|Proteomes:UP000241394};
RC TISSUE=Young leaf {ECO:0000313|EMBL:PSS23618.1};
RA Pilkington S., Crowhurst R., Hilario E., Nardozza S., Fraser L., Peng Y.,
RA Gunaseelan K., Simpson R., Tahir J., Deroles S., Templeton K., Luo Z.,
RA Davy M., Cheng C., Mcneilage M., Scaglione D., Liu Y., Zhang Q., Datson P.,
RA De Silva N., Gardiner S., Bassett H., Chagne D., Mccallum J., Dzierzon H.,
RA Deng C., Wang Y.-Y., Barron N., Manako K., Bowen J., Foster T., Erridge Z.,
RA Tiffin H., Waite C., Davies K., Grierson E., Laing W., Kirk R., Chen X.,
RA Wood M., Montefiori M., Brummell D., Schwinn K., Catanach A., Fullerton C.,
RA Li D., Meiyalaghan S., Nieuwenhuizen N., Read N., Prakash R., Hunter D.,
RA Zhang H., Mckenzie M., Knabel M., Harris A., Allan A., Chen A., Janssen B.,
RA Plunkett B., Dwamena C., Voogd C., Leif D., Lafferty D., Souleyre E.,
RA Varkonyi-Gasic E., Gambi F., Hanley J., Yao J.-L., Cheung J., David K.,
RA Warren B., Marsh K., Snowden K., Lin-Wang K., Brian L.,
RA Martinez-Sanchez M., Wang M., Ileperuma N., Macnee N., Campin R.,
RA Mcatee P., Drummond R., Espley R., Ireland H., Wu R., Atkinson R.,
RA Karunairetnam S., Bulley S., Chunkath S., Hanley Z., Storey R.,
RA Thrimawithana A., Thomson S., David C., Testolin R.;
RT "An improved, manually edited Actinidia chinensis var. chinensis
RT (kiwifruit) genome highlights the challenges associated with draft genomes
RT and gene prediction in plants.";
RL Submitted (JUL-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000241394}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Red5 {ECO:0000313|Proteomes:UP000241394};
RX PubMed=29661190; DOI=10.1186/s12864-018-4656-3;
RA Pilkington S.M., Crowhurst R., Hilario E., Nardozza S., Fraser L., Peng Y.,
RA Gunaseelan K., Simpson R., Tahir J., Deroles S.C., Templeton K., Luo Z.,
RA Davy M., Cheng C., McNeilage M., Scaglione D., Liu Y., Zhang Q., Datson P.,
RA De Silva N., Gardiner S.E., Bassett H., Chagne D., McCallum J.,
RA Dzierzon H., Deng C., Wang Y.Y., Barron L., Manako K., Bowen J.,
RA Foster T.M., Erridge Z.A., Tiffin H., Waite C.N., Davies K.M.,
RA Grierson E.P., Laing W.A., Kirk R., Chen X., Wood M., Montefiori M.,
RA Brummell D.A., Schwinn K.E., Catanach A., Fullerton C., Li D.,
RA Meiyalaghan S., Nieuwenhuizen N., Read N., Prakash R., Hunter D., Zhang H.,
RA McKenzie M., Knabel M., Harris A., Allan A.C., Gleave A., Chen A.,
RA Janssen B.J., Plunkett B., Ampomah-Dwamena C., Voogd C., Leif D.,
RA Lafferty D., Souleyre E.J.F., Varkonyi-Gasic E., Gambi F., Hanley J.,
RA Yao J.L., Cheung J., David K.M., Warren B., Marsh K., Snowden K.C.,
RA Lin-Wang K., Brian L., Martinez-Sanchez M., Wang M., Ileperuma N.,
RA Macnee N., Campin R., McAtee P., Drummond R.S.M., Espley R.V.,
RA Ireland H.S., Wu R., Atkinson R.G., Karunairetnam S., Bulley S.,
RA Chunkath S., Hanley Z., Storey R., Thrimawithana A.H., Thomson S.,
RA David C., Testolin R., Huang H., Hellens R.P., Schaffer R.J.;
RT "A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome
RT highlights the challenges associated with draft genomes and gene prediction
RT in plants.";
RL BMC Genomics 19:257-257(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate;
CC Xref=Rhea:RHEA:18157, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58702, ChEBI:CHEBI:456216;
CC EC=2.7.1.40; Evidence={ECO:0000256|ARBA:ARBA00001174,
CC ECO:0000256|RuleBase:RU000504};
CC -!- COFACTOR:
CC Name=K(+); Xref=ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|ARBA:ARBA00001958};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC glyceraldehyde 3-phosphate: step 5/5. {ECO:0000256|ARBA:ARBA00004997,
CC ECO:0000256|RuleBase:RU000504}.
CC -!- SIMILARITY: Belongs to the pyruvate kinase family.
CC {ECO:0000256|ARBA:ARBA00008663, ECO:0000256|RuleBase:RU000504}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PSS23618.1}.
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DR EMBL; NKQK01000008; PSS23618.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2R6R7T6; -.
DR STRING; 1590841.A0A2R6R7T6; -.
DR EnsemblPlants; PSS23618; PSS23618; CEY00_Acc08442.
DR Gramene; PSS23618; PSS23618; CEY00_Acc08442.
DR InParanoid; A0A2R6R7T6; -.
DR OMA; IVCREAE; -.
DR UniPathway; UPA00109; UER00188.
DR Proteomes; UP000241394; Chromosome lg8.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0030955; F:potassium ion binding; IEA:InterPro.
DR GO; GO:0004743; F:pyruvate kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR Gene3D; 2.40.33.10; PK beta-barrel domain-like; 1.
DR Gene3D; 3.40.1380.20; Pyruvate kinase, C-terminal domain; 1.
DR InterPro; IPR001697; Pyr_Knase.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR InterPro; IPR011037; Pyrv_Knase-like_insert_dom_sf.
DR InterPro; IPR015793; Pyrv_Knase_brl.
DR InterPro; IPR015795; Pyrv_Knase_C.
DR InterPro; IPR036918; Pyrv_Knase_C_sf.
DR InterPro; IPR015806; Pyrv_Knase_insert_dom_sf.
DR NCBIfam; TIGR01064; pyruv_kin; 1.
DR PANTHER; PTHR11817; PYRUVATE KINASE; 1.
DR PANTHER; PTHR11817:SF4; PYRUVATE KINASE; 1.
DR Pfam; PF00224; PK; 1.
DR Pfam; PF02887; PK_C; 1.
DR PRINTS; PR01050; PYRUVTKNASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR SUPFAM; SSF50800; PK beta-barrel domain-like; 1.
DR SUPFAM; SSF52935; PK C-terminal domain-like; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Glycolysis {ECO:0000256|ARBA:ARBA00023152, ECO:0000256|RuleBase:RU000504};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000256|RuleBase:RU000504};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|RuleBase:RU000504};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Pyruvate {ECO:0000256|ARBA:ARBA00023317, ECO:0000313|EMBL:PSS23618.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000241394};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000504}.
FT DOMAIN 30..366
FT /note="Pyruvate kinase barrel"
FT /evidence="ECO:0000259|Pfam:PF00224"
FT DOMAIN 401..521
FT /note="Pyruvate kinase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02887"
SQ SEQUENCE 533 AA; 58219 MW; EF9ECD9BA2607808 CRC64;
MHANHLLLEE PIRMATILEP SKASFFPAMT KIVGTMGPTS RSREVISGCL QAGMSVARFD
FSWGDPEYHQ ETLENLRAAV KTTKKLCAVM LDTVGPELLV VNNSDKTISL QEDGIVVLTP
DQGREASSDV LPINFSGLSK AVKKGDTIFV GQYLFTGSES TSVWLEVDEV KGQDVVCVIK
NTATLTGSLF TLHACQIRID LPTLSDKDKE VIGTWGFKNK IDFLSLSFTR HAEDVREARE
FLSNLGDISQ TQIFAKIENV EVSFNEMGLN HFDEILQEAD GIILSRGNLG IDLPPEKVFL
FQKAAVYKCN MAGKPAVVTR VVDSMTGNLR PTRAEATDVA NAVLDGTDAI LLGAETLRGL
YPIETISIVG KICAEAEKVF NQDLYFKKTV KYVGEPMSHL ESIASSAVRA AIKVKASVII
CFTSSGRAAR LIAKYRPTMP VISVVIPRLK TNQLKWSFSG AFEARQSLIV RGLFPMLADP
RHPAESTNAT NESVLKVALD HGKASGVIKS HDRVVICQKV GDSSVVKIIE LED
//
