ID A0A2R7YWL1_9ACTN Unreviewed; 490 AA.
AC A0A2R7YWL1;
DT 20-JUN-2018, integrated into UniProtKB/TrEMBL.
DT 20-JUN-2018, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE RecName: Full=DNA-3-methyladenine glycosylase II {ECO:0000256|ARBA:ARBA00012000};
DE EC=3.2.2.21 {ECO:0000256|ARBA:ARBA00012000};
GN ORFNames=C7S10_13555 {ECO:0000313|EMBL:PUA80765.1};
OS Nocardioides currus.
OC Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
OC Nocardioidaceae; Nocardioides.
OX NCBI_TaxID=2133958 {ECO:0000313|EMBL:PUA80765.1, ECO:0000313|Proteomes:UP000244867};
RN [1] {ECO:0000313|EMBL:PUA80765.1, ECO:0000313|Proteomes:UP000244867}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IB-3 {ECO:0000313|EMBL:PUA80765.1,
RC ECO:0000313|Proteomes:UP000244867};
RA Keele B.F.;
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-
CC methylguanine, 7-methylguanine and 7-methyladenine.; EC=3.2.2.21;
CC Evidence={ECO:0000256|ARBA:ARBA00000086};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PUA80765.1}.
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DR EMBL; PYXZ01000005; PUA80765.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2R7YWL1; -.
DR OrthoDB; 9811249at2; -.
DR Proteomes; UP000244867; Unassembled WGS sequence.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006284; P:base-excision repair; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd00056; ENDO3c; 1.
DR Gene3D; 3.40.10.10; DNA Methylphosphotriester Repair Domain; 1.
DR Gene3D; 3.30.310.20; DNA-3-methyladenine glycosylase AlkA, N-terminal domain; 1.
DR Gene3D; 1.10.1670.10; Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal); 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR InterPro; IPR035451; Ada-like_dom_sf.
DR InterPro; IPR004026; Ada_DNA_repair_Zn-bd.
DR InterPro; IPR010316; AlkA_N.
DR InterPro; IPR037046; AlkA_N_sf.
DR InterPro; IPR011257; DNA_glycosylase.
DR InterPro; IPR003265; HhH-GPD_domain.
DR InterPro; IPR023170; HhH_base_excis_C.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR018062; HTH_AraC-typ_CS.
DR PANTHER; PTHR43003; DNA-3-METHYLADENINE GLYCOSYLASE; 1.
DR PANTHER; PTHR43003:SF13; DNA-3-METHYLADENINE GLYCOSYLASE 2; 1.
DR Pfam; PF02805; Ada_Zn_binding; 1.
DR Pfam; PF06029; AlkA_N; 1.
DR Pfam; PF12833; HTH_18; 1.
DR SMART; SM01009; AlkA_N; 1.
DR SMART; SM00478; ENDO3c; 1.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF57884; Ada DNA repair protein, N-terminal domain (N-Ada 10); 1.
DR SUPFAM; SSF48150; DNA-glycosylase; 1.
DR SUPFAM; SSF46689; Homeodomain-like; 1.
DR SUPFAM; SSF55945; TATA-box binding protein-like; 1.
DR PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 4: Predicted;
KW Activator {ECO:0000256|ARBA:ARBA00023159};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000244867};
KW Transcription {ECO:0000256|ARBA:ARBA00023163};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 90..188
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000259|PROSITE:PS01124"
SQ SEQUENCE 490 AA; 52397 MW; 9D2EE36F0B4BC4CB CRC64;
MPRTDLDPES CYRAVKSKDR RFDGVFYTCV ATTGIYCRPS CPARTPGFAN VSFRPTAAAC
QAAGFRACKR CLPDATPGSP DWDVAADVAG RAMRLIADGL VDREGVDGLA SRVGYTPRHL
GRLLTQELGA GPLALARARR AQTARILIET TEMGFADIAF AAGFSSIRQF NETVREVYAV
SPSELRGRRG GRRTPSGTIT MRLAVRTPFA GSRLLDFLAF HVVPGVEVAG DGWYARTLDL
PHGPGTVRLE LGDDLRIGET AFVAAEFALE DVRDTAAAVE RVRRLLDADC DPVAVDDHLV
EDPVIGSLVR GTPGLRVPGQ VDGDETAVRT VIGQQVSVAG ARAVAGRIVA AHGRPVDTEV
PGLTHLFPDA AALAAVDPET LPMPRARGRA VVALATALSS GSLALDRGPD REDVRHRMLD
LPGIGPWTAD YVAMRALGHP DVFLAGDLAV RKVLAGLGGE AVDVEGWRPW RSYALMHLWN
TLMPSTSKEN
//
