ID A0A2R8ZNP9_PANPA Unreviewed; 901 AA.
AC A0A2R8ZNP9;
DT 20-JUN-2018, integrated into UniProtKB/TrEMBL.
DT 20-JUN-2018, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=Methionine--tRNA ligase, cytoplasmic {ECO:0000256|ARBA:ARBA00018335};
DE EC=6.1.1.10 {ECO:0000256|ARBA:ARBA00012838};
DE AltName: Full=Methionyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00030904};
GN Name=MARS1 {ECO:0000313|Ensembl:ENSPPAP00000006580.1};
OS Pan paniscus (Pygmy chimpanzee) (Bonobo).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Pan.
OX NCBI_TaxID=9597 {ECO:0000313|Ensembl:ENSPPAP00000006580.1, ECO:0000313|Proteomes:UP000240080};
RN [1] {ECO:0000313|Ensembl:ENSPPAP00000006580.1, ECO:0000313|Proteomes:UP000240080}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=22722832; DOI=10.1038/nature11128;
RA Prufer K., Munch K., Hellmann I., Akagi K., Miller J.R., Walenz B.,
RA Koren S., Sutton G., Kodira C., Winer R., Knight J.R., Mullikin J.C.,
RA Meader S.J., Ponting C.P., Lunter G., Higashino S., Hobolth A., Dutheil J.,
RA Karakoc E., Alkan C., Sajjadian S., Catacchio C.R., Ventura M.,
RA Marques-Bonet T., Eichler E.E., Andre C., Atencia R., Mugisha L.,
RA Junhold J., Patterson N., Siebauer M., Good J.M., Fischer A., Ptak S.E.,
RA Lachmann M., Symer D.E., Mailund T., Schierup M.H., Andres A.M., Kelso J.,
RA Paabo S.;
RT "The bonobo genome compared with the chimpanzee and human genomes.";
RL Nature 486:527-531(2012).
RN [2] {ECO:0000313|Ensembl:ENSPPAP00000006580.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-
CC methionyl-tRNA(Met); Xref=Rhea:RHEA:13481, Rhea:RHEA-COMP:9667,
CC Rhea:RHEA-COMP:9698, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:57844, ChEBI:CHEBI:78442, ChEBI:CHEBI:78530,
CC ChEBI:CHEBI:456215; EC=6.1.1.10;
CC Evidence={ECO:0000256|ARBA:ARBA00001234};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363039}.
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DR EMBL; AJFE02087625; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AJFE02087626; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AJFE02087627; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AJFE02087628; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AJFE02087629; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AJFE02087630; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AJFE02087631; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AJFE02087632; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AJFE02087633; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; A0A2R8ZNP9; -.
DR STRING; 9597.ENSPPAP00000006580; -.
DR Ensembl; ENSPPAT00000028025.1; ENSPPAP00000006580.1; ENSPPAG00000025147.1.
DR GeneTree; ENSGT00550000075017; -.
DR OMA; HLNTTEY; -.
DR Proteomes; UP000240080; Chromosome 12.
DR Bgee; ENSPPAG00000025147; Expressed in testis and 6 other cell types or tissues.
DR GO; GO:0017101; C:aminoacyl-tRNA synthetase multienzyme complex; IEA:Ensembl.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0005730; C:nucleolus; IEA:Ensembl.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004825; F:methionine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0071364; P:cellular response to epidermal growth factor stimulus; IEA:Ensembl.
DR GO; GO:0036120; P:cellular response to platelet-derived growth factor stimulus; IEA:Ensembl.
DR GO; GO:0006431; P:methionyl-tRNA aminoacylation; IEA:Ensembl.
DR GO; GO:1901838; P:positive regulation of transcription of nucleolar large rRNA by RNA polymerase I; IEA:Ensembl.
DR GO; GO:0009303; P:rRNA transcription; IEA:Ensembl.
DR CDD; cd07957; Anticodon_Ia_Met; 1.
DR CDD; cd10307; GST_C_MetRS_N; 1.
DR CDD; cd00814; MetRS_core; 1.
DR CDD; cd00939; MetRS_RNA; 1.
DR Gene3D; 1.20.1050.10; -; 1.
DR Gene3D; 3.40.30.10; Glutaredoxin; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 2.20.28.20; Methionyl-tRNA synthetase, Zn-domain; 1.
DR Gene3D; 1.10.287.10; S15/NS1, RNA-binding; 1.
DR HAMAP; MF_00098; Met_tRNA_synth_type1; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR041872; Anticodon_Met.
DR InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR InterPro; IPR004046; GST_C.
DR InterPro; IPR041598; MARS_N.
DR InterPro; IPR023458; Met-tRNA_ligase_1.
DR InterPro; IPR014758; Met-tRNA_synth.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR033911; MetRS_core.
DR InterPro; IPR029038; MetRS_Zn.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009068; uS15_NS1_RNA-bd_sf.
DR InterPro; IPR000738; WHEP-TRS_dom.
DR NCBIfam; TIGR00398; metG; 1.
DR PANTHER; PTHR45765; METHIONINE--TRNA LIGASE; 1.
DR PANTHER; PTHR45765:SF1; METHIONINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR Pfam; PF19303; Anticodon_3; 1.
DR Pfam; PF00043; GST_C; 1.
DR Pfam; PF18485; GST_N_5; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR Pfam; PF00458; WHEP-TRS; 1.
DR PRINTS; PR01041; TRNASYNTHMET.
DR SMART; SM00991; WHEP-TRS; 1.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF47616; GST C-terminal domain-like; 1.
DR SUPFAM; SSF57770; Methionyl-tRNA synthetase (MetRS), Zn-domain; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF47060; S15/NS1 RNA-binding domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
DR PROSITE; PS50405; GST_CTER; 1.
DR PROSITE; PS00762; WHEP_TRS_1; 1.
DR PROSITE; PS51185; WHEP_TRS_2; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363039};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363039};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363039};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363039};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363039};
KW Reference proteome {ECO:0000313|Proteomes:UP000240080}.
FT DOMAIN 74..191
FT /note="GST C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS50405"
FT DOMAIN 842..898
FT /note="WHEP-TRS"
FT /evidence="ECO:0000259|PROSITE:PS51185"
SQ SEQUENCE 901 AA; 101477 MW; 0D447C3FCA733EBD CRC64;
MRLFVSEGVP GCLPVLAAAG RARGRAEVLI STVGPEDCVV PFLTRPKVPV LQLDSGNYLF
STSAICRYFF LLSGWEQDDL TNQWLEWEAT ELQPALSAAL QEGEDVLGSV RRALTHIDHS
LSRQNCPFLA GETESLADIV LWGALYPLLQ DPAYLPEELS ALHSWFQTLS TQEPCQRAAE
TVLKQQGVLA LRPYLQKQPQ PSPTEGRAVT NEPEEEELAT LSEEEIAMAV TAWEKGLESL
PPLRPQQNPV LPVAGERNVL ITSALPYVNN VPHLGNIIGC VLSADVFARY SRLRQWNTLY
LCGTDEYGTA TETKALEEGL TPQEICDKYH IIHADIYRWF NISFDIFGRT TTPQQTKITQ
DIFQQLLKRG FVWQDTVEQL RCEHCARFLA DRFVEGMCPF CGYEEARGDQ CDKCGKLINA
VELKKPQCKV CRSCPVVQST QHLFLDLPKL EKRLEEWLGR TLPGSDWTPN AQFITRSWLR
DGLKPRCITR DLKWGTPVPL EGFEDKVFYV WFDATIGYLS ITANYTDQWE RWWKNPEQVD
LYQFMAKDNV PFHSLVFPCS ALGAEDNYTL VSHLIATEYL NYEDGKFSKS RGVGVFGDMA
QDTGIPADIW RFYLLYIRPE GQDSAFSWTD LLLKNNSELL NNLGNFINRA GMFVSKFFGG
YVPEMVLTPD DQRLLAHVTL ELQHYHQLLE KVRIRDALRS ILTISRHGNQ YIQVNEPWKR
IKGSEADRQR AGTVTGLAVN IAALLSVMLQ PYMPTVSATI QAQLQLPPPA CSILLTNFLC
TLPAGHQIGT VSPLFQKLEN DQIESLRQRF GGGQLEETFD LKAKTSLKPA VVETVTTARP
QQIQALMDEV TKQGNIVREL KAQKADKNEV AAEVAKLLDL KKQLAVAEGK PPEAPKGKKK
K
//
