ID A0A2R9B733_PANPA Unreviewed; 613 AA.
AC A0A2R9B733;
DT 20-JUN-2018, integrated into UniProtKB/TrEMBL.
DT 20-JUN-2018, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=Cytidine monophosphate-N-acetylneuraminic acid hydroxylase {ECO:0000256|ARBA:ARBA00015403};
DE EC=1.14.18.2 {ECO:0000256|ARBA:ARBA00011904};
DE AltName: Full=CMP-N-acetylneuraminate monooxygenase {ECO:0000256|ARBA:ARBA00033362};
DE AltName: Full=CMP-Neu5Ac hydroxylase {ECO:0000256|ARBA:ARBA00030460};
DE AltName: Full=CMP-NeuAc hydroxylase {ECO:0000256|ARBA:ARBA00029883};
OS Pan paniscus (Pygmy chimpanzee) (Bonobo).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Pan.
OX NCBI_TaxID=9597 {ECO:0000313|Ensembl:ENSPPAP00000024035.1, ECO:0000313|Proteomes:UP000240080};
RN [1] {ECO:0000313|Ensembl:ENSPPAP00000024035.1, ECO:0000313|Proteomes:UP000240080}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=22722832; DOI=10.1038/nature11128;
RA Prufer K., Munch K., Hellmann I., Akagi K., Miller J.R., Walenz B.,
RA Koren S., Sutton G., Kodira C., Winer R., Knight J.R., Mullikin J.C.,
RA Meader S.J., Ponting C.P., Lunter G., Higashino S., Hobolth A., Dutheil J.,
RA Karakoc E., Alkan C., Sajjadian S., Catacchio C.R., Ventura M.,
RA Marques-Bonet T., Eichler E.E., Andre C., Atencia R., Mugisha L.,
RA Junhold J., Patterson N., Siebauer M., Good J.M., Fischer A., Ptak S.E.,
RA Lachmann M., Symer D.E., Mailund T., Schierup M.H., Andres A.M., Kelso J.,
RA Paabo S.;
RT "The bonobo genome compared with the chimpanzee and human genomes.";
RL Nature 486:527-531(2012).
RN [2] {ECO:0000313|Ensembl:ENSPPAP00000024035.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Sialic acids are components of carbohydrate chains of
CC glycoconjugates and are involved in cell-cell recognition and cell-
CC pathogen interactions. Catalyzes the conversion of CMP-N-
CC acetylneuraminic acid (CMP-Neu5Ac) into its hydroxylated derivative
CC CMP-N-glycolylneuraminic acid (CMP-Neu5Gc), a sialic acid abundantly
CC expressed at the surface of many cells.
CC {ECO:0000256|ARBA:ARBA00003414}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=CMP-N-acetyl-beta-neuraminate + 2 Fe(II)-[cytochrome b5] + 2
CC H(+) + O2 = CMP-N-glycoloyl-beta-neuraminate + 2 Fe(III)-[cytochrome
CC b5] + H2O; Xref=Rhea:RHEA:16145, Rhea:RHEA-COMP:10438, Rhea:RHEA-
CC COMP:10439, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:29033, ChEBI:CHEBI:29034, ChEBI:CHEBI:57812,
CC ChEBI:CHEBI:58376; EC=1.14.18.2;
CC Evidence={ECO:0000256|ARBA:ARBA00029339};
CC -!- PATHWAY: Amino-sugar metabolism; N-acetylneuraminate metabolism.
CC {ECO:0000256|ARBA:ARBA00005141}.
CC -!- SIMILARITY: Belongs to the CMP-Neu5Ac hydroxylase family.
CC {ECO:0000256|ARBA:ARBA00010303}.
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DR EMBL; AJFE02081283; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AJFE02081284; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AJFE02081285; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_008975058.1; XM_008976810.1.
DR AlphaFoldDB; A0A2R9B733; -.
DR Ensembl; ENSPPAT00000046860.1; ENSPPAP00000024035.1; ENSPPAG00000035155.1.
DR GeneID; 100978297; -.
DR KEGG; pps:100978297; -.
DR CTD; 12763; -.
DR GeneTree; ENSGT00390000010830; -.
DR OMA; WKSFLMC; -.
DR OrthoDB; 5385472at2759; -.
DR UniPathway; UPA00628; -.
DR Proteomes; UP000240080; Chromosome 6.
DR Bgee; ENSPPAG00000035155; Expressed in liver and 4 other cell types or tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:Ensembl.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:InterPro.
DR GO; GO:0030338; F:CMP-N-acetylneuraminate monooxygenase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0046381; P:CMP-N-acetylneuraminate metabolic process; IEA:Ensembl.
DR GO; GO:0006054; P:N-acetylneuraminate metabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd03473; Rieske_CMP_Neu5Ac_hydrolase_N; 1.
DR Gene3D; 3.60.15.10; Ribonuclease Z/Hydroxyacylglutathione hydrolase-like; 1.
DR Gene3D; 2.102.10.10; Rieske [2Fe-2S] iron-sulphur domain; 1.
DR InterPro; IPR037339; CMP-Neu5Ac_hydroxylase_Rieske.
DR InterPro; IPR027033; Cnh.
DR InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR InterPro; IPR017941; Rieske_2Fe-2S.
DR InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR PANTHER; PTHR46522; CYTIDINE MONOPHOSPHATE-N-ACETYLNEURAMINIC ACID HYDROXYLASE; 1.
DR PANTHER; PTHR46522:SF3; INACTIVE CYTIDINE MONOPHOSPHATE-N-ACETYLNEURAMINIC ACID HYDROXYLASE; 1.
DR Pfam; PF13483; Lactamase_B_3; 1.
DR Pfam; PF00355; Rieske; 1.
DR SUPFAM; SSF50022; ISP domain; 1.
DR SUPFAM; SSF56281; Metallo-hydrolase/oxidoreductase; 1.
DR PROSITE; PS51296; RIESKE; 1.
PE 3: Inferred from homology;
KW 2Fe-2S {ECO:0000256|ARBA:ARBA00022714};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000240080}.
FT DOMAIN 37..135
FT /note="Rieske"
FT /evidence="ECO:0000259|PROSITE:PS51296"
SQ SEQUENCE 613 AA; 71187 MW; 00614A2D5214DCF5 CRC64;
MSNRNDTSNC CSFKIPRQRN RQTMGSIEQT TEILLCLSPV EVASLKEGIN FFRNKSTGKD
YILYKNKSRL RACKNMCKHQ GGLFIKDIED LAGRSVRCTK HNWKLDVSTM KYINPPESFC
QDELVVEMDE NNRLLLLELN PPNPWDLQPR SPEELAFGEV QITYLTHACM DLKLGDKRMV
FDPWLIGPAF ARGWWLLHEP PSDWLERLCQ ADLIYISHLH SDHLSYPTLK KLAGRRPDIP
IYVGNTERPV FWNLNQSGVQ LTNINVVPFG IWQQVDKNLR FMILMDGVHP EMDTCIIVEY
KGHKILNTVD CTRPNGGRLP MKVALMMSDF AGGASGFPMT FSGGKFTEEW KAQFIKTERK
KLLNYKARLV KNLQPRIYCP FAGYFVESHP SDKYIKETNT KNDPNELNNL IKKNSDVITW
TPRPGATLDL GRMLKDPTDS KGIIEPPEGT KIYKDSWDFE PYLEILNAAV GDEIFLHSSW
IKEYFTWAGF KDYNLVVRMI ETDEDFNPFP GGYDYLVDFL DLSFPKERPQ REHPYEEIHS
RVDVIRHVVK NGLLWDELYI GFQTRLQRDP DIYHHLFWNH FQIKLPLTPP NWKSFLMCCE
QNGPGILQEC KTT
//
