ID   A0A2S0N3A9_9BURK        Unreviewed;       932 AA.
AC   A0A2S0N3A9;
DT   18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT   18-JUL-2018, sequence version 1.
DT   24-JAN-2024, entry version 18.
DE   RecName: Full=type I site-specific deoxyribonuclease {ECO:0000256|ARBA:ARBA00012654};
DE            EC=3.1.21.3 {ECO:0000256|ARBA:ARBA00012654};
GN   ORFNames=C6571_14900 {ECO:0000313|EMBL:AVO42403.1};
OS   Simplicispira suum.
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Simplicispira.
OX   NCBI_TaxID=2109915 {ECO:0000313|EMBL:AVO42403.1, ECO:0000313|Proteomes:UP000239326};
RN   [1] {ECO:0000313|EMBL:AVO42403.1, ECO:0000313|Proteomes:UP000239326}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC1-8 {ECO:0000313|EMBL:AVO42403.1,
RC   ECO:0000313|Proteomes:UP000239326};
RA   Kim S.-J., Heo J., Kwon S.-W.;
RT   "Genome sequencing of Simplicispira sp.";
RL   Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give random double-stranded
CC         fragments with terminal 5'-phosphates, ATP is simultaneously
CC         hydrolyzed.; EC=3.1.21.3; Evidence={ECO:0000256|ARBA:ARBA00000851};
CC   -!- SIMILARITY: Belongs to the HsdR family.
CC       {ECO:0000256|ARBA:ARBA00008598}.
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DR   EMBL; CP027669; AVO42403.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2S0N3A9; -.
DR   REBASE; 244289; SspSC18IP.
DR   KEGG; simp:C6571_14900; -.
DR   OrthoDB; 9804086at2; -.
DR   Proteomes; UP000239326; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009035; F:type I site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   CDD; cd18032; DEXHc_RE_I_III_res; 1.
DR   CDD; cd18799; SF2_C_EcoAI-like; 1.
DR   Gene3D; 3.90.1570.30; -; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR   InterPro; IPR013670; EcoEI_R_C_dom.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR007409; Restrct_endonuc_type1_HsdR_N.
DR   PANTHER; PTHR47396:SF1; ATP-DEPENDENT HELICASE IRC3-RELATED; 1.
DR   PANTHER; PTHR47396; TYPE I RESTRICTION ENZYME ECOKI R PROTEIN; 1.
DR   Pfam; PF08463; EcoEI_R_C; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF04313; HSDR_N; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW   Endonuclease {ECO:0000256|ARBA:ARBA00022759, ECO:0000313|EMBL:AVO42403.1};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Restriction system {ECO:0000256|ARBA:ARBA00022747}.
FT   DOMAIN          173..329
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS51192"
SQ   SEQUENCE   932 AA;  106911 MW;  D056893B05FC424F CRC64;
     MTYSEAATRS ALIDNALGQS GWNVKDPTQV VEEFDIKVGL APGVKEPRTP YEGHHFSDYV
     LLGKDGKPLA VVEAKRTSKD AALGREQAKQ YCYNIQKQLG GELPFCFYTN GHDTYFWDLE
     NTPPRKVVGF PTRDDLERFS YIRRNRKPLT HEFINTSIAG RDYQIRAIRS VLEGIEQKKR
     DFLLVMATGT GKTRTCIALV DALMRAGHAE KVLFLVDRIA LREQALAAFK EHMPNEPRWP
     NPGEKLVAKD RRVYVATYPT MLNVIRDEAQ HLSPHFFDFI VVDESHRSIY NTFGEVLDYF
     KTITVGLTAT PTALIDHNTF QLFHCEDGIP TFAYTYEEAV NNFPPYLSNF QVMKIQTKFQ
     MEGISKRTIS LEDQKRLILQ GKDVEEINFE GSQLEKQVIN KGTNTLIVRE FMEESIKDAN
     GVLPGKTIFF CATKAHARRM EEIFDKLYPQ YHGELAKVLV SDDPRVYGKG GLLDQFTNND
     MPRIAISVDM LDTGIDVREI VNLVFAKPVY SYTKFWQMVG RGTRLLETNK PKPWCLEKDV
     FLILDCWDNF EYFKLNPKGR ELKPQLSLPV RLVGLRLDKI ERANDSEHAE TAAREVTKLR
     SQIAALPNES VVIKEAAAVL ARLQDENFWI SLNHDRLEFL RAEVKPLFRT VSEADFKAMR
     FERDVLEYSL AVLSEAKEQA ETLKDGIVEQ IVELPLSVSF VKKEEALIRA AQTNHYWAKA
     DEDAFDELVA KLGPLMKFRE HNTGQEQTHL DLADELHKKE RVEFGPQHES VSITRYREMV
     EALVTELTDY NPVLQKIKNG EPISAEEASQ LADLLHEEHP HITEDLLRQV YKNRKARFIQ
     FIRHILGIEV LRSFPDEVSA AFDQFIRAHT TLSSRQMEFL NLLKGFIIER EKVEKKDLIN
     APFTVIHPQG IRGVFSPLEI NEILQLTERL AA
//