ID A0A2S0P8H9_9NEIS Unreviewed; 718 AA.
AC A0A2S0P8H9;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN ORFNames=DAI18_05705 {ECO:0000313|EMBL:AVY93597.1};
OS Microvirgula aerodenitrificans.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
OC Aquaspirillaceae; Microvirgula.
OX NCBI_TaxID=57480 {ECO:0000313|EMBL:AVY93597.1, ECO:0000313|Proteomes:UP000244173};
RN [1] {ECO:0000313|EMBL:AVY93597.1, ECO:0000313|Proteomes:UP000244173}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BE2.4 {ECO:0000313|EMBL:AVY93597.1,
RC ECO:0000313|Proteomes:UP000244173};
RA Anderson E., Jang J., Ishii S.;
RT "Denitrifier Microvirgula.";
RL Submitted (APR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; CP028519; AVY93597.1; -; Genomic_DNA.
DR RefSeq; WP_028498571.1; NZ_CP028519.1.
DR AlphaFoldDB; A0A2S0P8H9; -.
DR STRING; 1122240.GCA_000620105_01196; -.
DR OrthoDB; 5905204at2; -.
DR Proteomes; UP000244173; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000244173}.
FT DOMAIN 7..283
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 284..550
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 27..34
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 718 AA; 79419 MW; 139F931FC8ABE77E CRC64;
MNSEFLAGLN PAQREAVTTP HSALVLAGAG SGKTRVLTTR IAWLIREGLA SPAGILGVTF
TNKAAREMQT RLTAMLPVNP KQMWIGTFHG LANRLLRLHH RDVGLPATFQ ILDSQDQLAA
IKRLLKTLQL SDETYPPRQV MSFINGSKEK GLRADRFAPA DHFEVKLTEI WREYDRQCRR
EGVVDFAELL LASYELLSGN TALRQHYQSR FRHILVDEFQ DTNRLQYAWL KCFAGDDNTV
FAVGDDDQSI YAFRGAEVGN MRDFLSDYAA GEPIRLEQNY RSTSRILAAA NAVIANNRGR
LGKTLWTDAG EGEQIRVYRG WSDGEEAEFI AEEARALKRE GVSLADVAVL YRSNAQSRAL
EHALFQAGLS YRVYGGLRFF ERQEIKHALA YLRLLANPAD DNALLRIINL PARGIGARTV
DGIQMAARDS GDSLWQAACQ VGGRAAASVG RFVLLIEELR TAADTLGFAE LVSHVIDASG
LTAMYQADKD GSERLENLSE LVNAAVSFVP DDPAQAMVEF LAHASLESGD HEAAAGEEAI
TLMTVHAAKG LEFDSVFLTG LEEGLFPHEQ SFADPDGLEE ERRLLYVAIT RARRRLTLSY
AQTRMLHGQT RYPLPSRFLD ELPPELLHPI NIEPVVRKPV ETAAPWAPTG RKTRTPVRTA
APEWVGGFRV GQTVTHPKFG LGVITDAEAG GGESRVEVNF QDAGRKWLDL RYAKLSAV
//
