ID   A0A2S0PC37_9NEIS        Unreviewed;      1145 AA.
AC   A0A2S0PC37;
DT   18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT   18-JUL-2018, sequence version 1.
DT   27-MAR-2024, entry version 21.
DE   RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE            EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN   ORFNames=DAI18_13275 {ECO:0000313|EMBL:AVY94902.1};
OS   Microvirgula aerodenitrificans.
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
OC   Aquaspirillaceae; Microvirgula.
OX   NCBI_TaxID=57480 {ECO:0000313|EMBL:AVY94902.1, ECO:0000313|Proteomes:UP000244173};
RN   [1] {ECO:0000313|EMBL:AVY94902.1, ECO:0000313|Proteomes:UP000244173}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BE2.4 {ECO:0000313|EMBL:AVY94902.1,
RC   ECO:0000313|Proteomes:UP000244173};
RA   Anderson E., Jang J., Ishii S.;
RT   "Denitrifier Microvirgula.";
RL   Submitted (APR-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC         Evidence={ECO:0000256|ARBA:ARBA00024632};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR   EMBL; CP028519; AVY94902.1; -; Genomic_DNA.
DR   RefSeq; WP_028498948.1; NZ_CP028519.1.
DR   AlphaFoldDB; A0A2S0PC37; -.
DR   STRING; 1122240.GCA_000620105_01630; -.
DR   OrthoDB; 9803237at2; -.
DR   Proteomes; UP000244173; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   CDD; cd04485; DnaE_OBF; 1.
DR   CDD; cd07433; PHP_PolIIIA_DnaE1; 1.
DR   Gene3D; 1.10.150.870; -; 1.
DR   Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR   Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR048472; DNA_pol_IIIA_C.
DR   InterPro; IPR004805; DnaE2/DnaE/PolC.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR049821; PolIIIA_DnaE1_PHP.
DR   NCBIfam; TIGR00594; polc; 1.
DR   PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR   PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF20914; DNA_pol_IIIA_C; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; PHP domain-like; 1.
PE   4: Predicted;
KW   DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW   DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW   Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW   Reference proteome {ECO:0000313|Proteomes:UP000244173};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          7..74
FT                   /note="Polymerase/histidinol phosphatase N-terminal"
FT                   /evidence="ECO:0000259|SMART:SM00481"
SQ   SEQUENCE   1145 AA;  128387 MW;  BE3EEDD4EB1EC718 CRC64;
     MTTPRFVHLR LHTEFSVTDG ITRVDDAVKR AVGERMPALG ISDLMNLFGM VKHYKACLKQ
     GVKPIIGVDA WLENETDRDQ PFRLLLIAMN RTGYHRLCEL MTDAYRTNQY RGRAELKREW
     LDAASCEHLI LVSGAQFGDV GQALLAGNLE QARQRATEWQ TRFPGRCYLE IQRIGKPEVE
     TCVDRTLWLA GETGLPVVAT HPIQFLDQDD FKAHEARVCI AEGEMLGNPK RPRRYTDQQY
     FKSADDMAAL FADVPEALEN SVEIARRCNI EIELGKNYLP LFPTPAGMTL DDYLVHRAEE
     GLVERMQALY PDEALREQNM PRYRDRLKFE LDTIIQMGFP GYFLIVADFI NWAKGNGCPV
     GPGRGSGAGS LVAFSIGITD IDPLAYALLF ERFLNPERVS MPDFDIDFCQ DNRWRVIEYV
     REKYGADAVS QIATFGTMAA KAVVRDVGRV LDLPYSFCDG LSKLIPAAPG KQYSLDDAVE
     MEPTLKERIA NEEEVAELWT LAKKLEGVTR NIGMHAGGVL IAPGQIVDFC PMYQASGDDA
     SPVSMLDKGD VEEVGLVKFD FLGLRNLTII ELAVEYIHKL DPKFDIDLNH MGFDDVAAYR
     ILQQANTTAV FQLESDGMKK LLEKLKPDRF EDIIAVLALY RPGPLGSGMV DDFILRKAGK
     QAVDYFHPDL TACLDPTYGV IVYQEQVMQI SQIIGGYTLG GADMLRRAMG KKKAEEMDKH
     RGLFVAGALK NGYKQELAEY LFDLMAKFAE YGFNKSHTAA YAVVSYQTAW LKAHYPSAFM
     AATMSSELDN TDQLRVFYDD SLKNGLVFLP PDVNEGFYRF VPTDGKHIRY ALGAVKGTGE
     AAVEHIVEVR REGRFTDLFD FCRRTDKRLV NRRTIEALIR AGAFDAIDDN RAKLLANVEL
     AMSVAEQEAE NINQGGLFDM FEAEDVPTVD MVDVKPWDEA TRLTEEKTAI GFFLSGHPFH
     AYESTVRRFI RTPLNRLSPK KEPQWLAGTI TGIRTKIGNR GKMAFIQLDD GSAKVEVSIF
     SEAFDEARHK LKEDVVLVVS GKVSHDDFTG GMRIVADTVF DLAEAKSQFA KALTVDMPSS
     RADLNRLRTL ISPHVGGGCP LRIDYRNADA RCLLQLGDDW RVKLDDGMLA ALREWLGEQA
     VKVVF
//