ID A0A2S0U9H7_9BACL Unreviewed; 656 AA.
AC A0A2S0U9H7;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 13.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN Name=pulA {ECO:0000313|EMBL:AWB44472.1};
GN ORFNames=DCC85_09705 {ECO:0000313|EMBL:AWB44472.1};
OS Paenibacillus sp. CAA11.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=1532905 {ECO:0000313|EMBL:AWB44472.1, ECO:0000313|Proteomes:UP000244407};
RN [1] {ECO:0000313|EMBL:AWB44472.1, ECO:0000313|Proteomes:UP000244407}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CAA11 {ECO:0000313|EMBL:AWB44472.1,
RC ECO:0000313|Proteomes:UP000244407};
RA Um Y., Kim S., Gong G.;
RT "Complete genome of CAA11.";
RL Submitted (APR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
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DR EMBL; CP028922; AWB44472.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2S0U9H7; -.
DR OrthoDB; 9761875at2; -.
DR Proteomes; UP000244407; Chromosome.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR049117; pulA_all-beta.
DR InterPro; IPR011840; PulA_typeI.
DR NCBIfam; TIGR02104; pulA_typeI; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF21653; pulA_all-beta; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000244407}.
FT DOMAIN 182..556
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 656 AA; 74565 MW; 3F5C8A73426632B1 CRC64;
MSVQKERDIV IDYGDPVITG GISVFDPEFD KAYYYDGNDL GATYSPKRTQ FRLWAPTASE
AWVFLYDSWD DDRPDKRRMR RDVKGTWILS IDEDLQNRYY TYCVHVGEQI NEAVDPYAKA
VGVNGDRGAI IDLQKTNPAG WTEEKPPLES PLDAIIYEVH LRDLSIHPAS GIEHKGKYLG
LAEGGTKGPG GISTGLDHIS NLGVTHVQIL PFYDYSTESV DETRLDEPQY NWGYDPKNYN
VPEGSYATDP YKPAVRIAEL KQMIQALHDR GLRVVMDVVY NHVYDGYIVN FTRLVPGYYL
RYTPDHKFSN GSGCGNDVAS ERRMVTKFIV DSVLYWAREY HIDGFRFDLM GLLDVNTMNE
LRRRLDEVDP SILLYGEGWM MDTALPPRQR ANQQQAGRMP KIGHFNDVIR NALKGHVFEY
TAQGFVTGGK GLEDEVRKGV VGGIAYNDDI VSFVEEPVQS INYVECHDNH TLWDKIMLST
GTDSNLLRPA MHRLASAIIL TSQGIPFIHA GQEFLRTKDG VENSYKSEDL VNRLDWDRCN
VHQEDVQFMK RLIELRKEHA AFRLRDADSI RKHLVFEKSP KQTVAFTLRN HAGGDQDKHL
YVAYSACVKG AELDLPQIGT WEIQFGEELV SNFGEELQEK RLQIKGIGMV VLAVKA
//
