ID A0A2S1LSR0_9FLAO Unreviewed; 348 AA.
AC A0A2S1LSR0;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU362028};
DE EC=5.4.99.- {ECO:0000256|RuleBase:RU362028};
GN ORFNames=FK004_16960 {ECO:0000313|EMBL:AWG26800.1};
OS Flavobacterium kingsejongi.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=1678728 {ECO:0000313|EMBL:AWG26800.1, ECO:0000313|Proteomes:UP000244677};
RN [1] {ECO:0000313|EMBL:AWG26800.1, ECO:0000313|Proteomes:UP000244677}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AJ004 {ECO:0000313|EMBL:AWG26800.1,
RC ECO:0000313|Proteomes:UP000244677};
RA Lee P.C.;
RT "Complete genome sequence of Flavobacterium kingsejong AJ004.";
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil.
CC {ECO:0000256|RuleBase:RU362028}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC Evidence={ECO:0000256|RuleBase:RU362028};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000256|ARBA:ARBA00010876, ECO:0000256|RuleBase:RU362028}.
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DR EMBL; CP020919; AWG26800.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2S1LSR0; -.
DR KEGG; fki:FK004_16960; -.
DR OrthoDB; 9807829at2; -.
DR Proteomes; UP000244677; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006224; PsdUridine_synth_RluA-like_CS.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR NCBIfam; TIGR00005; rluA_subfam; 1.
DR PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1.
DR PANTHER; PTHR21600:SF44; PSEUDOU_SYNTH_2 DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|RuleBase:RU362028};
KW Reference proteome {ECO:0000313|Proteomes:UP000244677};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT DOMAIN 30..90
FT /note="RNA-binding S4"
FT /evidence="ECO:0000259|SMART:SM00363"
FT ACT_SITE 153
FT /evidence="ECO:0000256|PIRSR:PIRSR606225-1"
SQ SEQUENCE 348 AA; 39382 MW; 6594D5F058F21889 CRC64;
MDQFPTTDVD LEDELFEHFK FEASKGQSPL RVDKYLMGLI SNATRNKIQN AATSGNIFVN
DVTVKSNYKV KAGDVVKVML SHPPFENNVE PENIPLDVVY EDDALLIVNK PAGLVVHPGH
GNYTGTLVNA LAYHFENLPM NSSERPGLVH RIDKDTTGLL VVAKTEAAMT HLAKQFEAKT
SEREYVALVW GNVVEEEGTI TGNIARHVKD RMQMAVFADE EIGKHAVTHY KVLERFGYVT
LISCKLETGR THQIRVHLKH IGHTLFNDAR YGGDIILKGT TFTKYKQFID NCFKILPRQA
LHAKTLGFVH PVTGEMMRFD TELPQDMKDI LEKWRTYSKS HTVEEEED
//
