ID A0A2S1YX69_9ACTN Unreviewed; 963 AA.
AC A0A2S1YX69;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000256|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000256|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000256|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000256|HAMAP-Rule:MF_00049};
GN ORFNames=DDQ41_06450 {ECO:0000313|EMBL:AWK08622.1}, SSP531S_15030
GN {ECO:0000313|EMBL:GBQ00093.1};
OS Streptomyces spongiicola.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1690221 {ECO:0000313|EMBL:AWK08622.1, ECO:0000313|Proteomes:UP000245051};
RN [1] {ECO:0000313|EMBL:AWK08622.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HNM0071 {ECO:0000313|EMBL:AWK08622.1};
RA Zhou S., Huang X.;
RT "Complete genome sequence of the Type Strain of Streptomyces spongiicola
RT HNM0071, the producer of staurosporine.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:GBQ00093.1, ECO:0000313|Proteomes:UP000265354}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=531S {ECO:0000313|EMBL:GBQ00093.1,
RC ECO:0000313|Proteomes:UP000265354};
RA Dohra H., Kodani S.;
RT "Whole Genome Shotgun Sequence of Streptomyces spongiicola strain 531S.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00001372, ECO:0000256|HAMAP-
CC Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|HAMAP-Rule:MF_00049,
CC ECO:0000256|RuleBase:RU363039}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_00049}.
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DR EMBL; CP029254; AWK08622.1; -; Genomic_DNA.
DR EMBL; BGZL01000003; GBQ00093.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2S1YX69; -.
DR KEGG; sspo:DDQ41_06450; -.
DR OrthoDB; 9810365at2; -.
DR Proteomes; UP000245051; Chromosome.
DR Proteomes; UP000265354; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR CDD; cd07958; Anticodon_Ia_Leu_BEm; 1.
DR Gene3D; 3.40.50.620; HUPs; 3.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00396; leuS_bact; 1.
DR PANTHER; PTHR43740:SF2; LEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43740; LEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|HAMAP-Rule:MF_00049};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00049};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00049};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|HAMAP-Rule:MF_00049};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00049};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00049}.
FT DOMAIN 75..157
FT /note="Methionyl/Leucyl tRNA synthetase"
FT /evidence="ECO:0000259|Pfam:PF09334"
FT DOMAIN 304..506
FT /note="Leucyl-tRNA synthetase editing"
FT /evidence="ECO:0000259|Pfam:PF13603"
FT DOMAIN 809..922
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 560..582
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 734..738
FT /note="'KMSKS' region"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00049"
FT COMPBIAS 562..579
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 737
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00049"
SQ SEQUENCE 963 AA; 106363 MW; DD0BD889EE4E2AB7 CRC64;
MSEKNSSPVA SGTDGATSAE AAAPHRYTAA AAADIEARWQ DFWDADGTYE APNPSGDLAG
DAGVVARPKK FIMDMFPYPS GAGLHVGHPL GYIATDAYAR YQRMTGHNVL HTLGFDAFGL
PAEQYAVQTG THPRVSTEAN IENMKAQLRR LGLGHDRRRS FATIDPDYYK WTQWIFLRIF
GSWYDEAAGK ARPIEDLAAQ FESGERATPS GRPWSELSAV ERADVLSGHR LAYASDSPVN
WCPGLGTVLA NEEVTAEGRS ERGNFPVFKA NLRQWNMRIT AYADRLLDDL DALDWPEAIK
LQQRNWIGRS EGARVDFPVD GHDHARVTVF TTRPDTVFGA TYMVLAPEHD LIDSVLPAEW
PEDARGRDAW TGGAATPAEA VAEYRKQAQT KSDVERQTEH KAKTGVFTGA YAVNPVTGAR
IPVFVADYVL MGYGTGAIMA VPGQDERDWE FAEAFGLPIV RTVRPPEGWE GEAFTGQGPA
INSSNDEISL DGLEVAEAKA KITEWLVAKG VGEATVNFRL RDWLFSRQRY WGEPFPIVYD
EDGVAHALPE SMLPLELPEV DDYSPRTFDP DDADTRPETP LSRNAEWVEV ELDLGDGVKR
YRRETNTMPN WAGSCWYELR YLDPHNADRL VDPAIERYWM GPREGQPHGG VDLYVGGAEH
AVLHLLYARF WSKVLYDLGH VSSSEPFHKL YNQGMIQAHV YRDARGFPVP AAEVEERDGG
YVHGGEPVRR ELGKMGKSLK NAVTPDEIAA EYGADTLRLY EMAMGPLDVS RPWDTRAVVG
QYRLLQRLWR NVVDETTGEV TVVDAEPDEA TLRALHKTVD GVRQDMEAMR FNTAIAKITE
LNNHLTRTGG PVARSVAERL VLLIAPLAPH IGEELWRRLG HSGSVVHQDF PVADPAYVVD
ETVTCVVQVK GKVKARLEVS PSIGDAELEA LAVDDPAVVA ALGGAEVRKV IVRAPKLVNI
VPA
//
