ID A0A2S2CYN8_9PROT Unreviewed; 856 AA.
AC A0A2S2CYN8;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=phosphoenolpyruvate--protein phosphotransferase {ECO:0000256|ARBA:ARBA00012232};
DE EC=2.7.3.9 {ECO:0000256|ARBA:ARBA00012232};
GN Name=ptsP {ECO:0000313|EMBL:AWK89624.1};
GN ORFNames=DEW08_26860 {ECO:0000313|EMBL:AWK89624.1};
OS Azospirillum thermophilum.
OG Plasmid unnamed2 {ECO:0000313|EMBL:AWK89624.1,
OG ECO:0000313|Proteomes:UP000245629}.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC Azospirillaceae; Azospirillum.
OX NCBI_TaxID=2202148 {ECO:0000313|EMBL:AWK89624.1, ECO:0000313|Proteomes:UP000245629};
RN [1] {ECO:0000313|Proteomes:UP000245629}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CFH 70021 {ECO:0000313|Proteomes:UP000245629};
RC PLASMID=unnamed2 {ECO:0000313|Proteomes:UP000245629};
RA Zhao Z.;
RT "Azospirillum thermophila sp. nov., a novel isolated from hot spring.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-
CC L-histidyl-[protein] + pyruvate; Xref=Rhea:RHEA:23880, Rhea:RHEA-
CC COMP:9745, Rhea:RHEA-COMP:9746, ChEBI:CHEBI:15361, ChEBI:CHEBI:29979,
CC ChEBI:CHEBI:58702, ChEBI:CHEBI:64837; EC=2.7.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00000683};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the PEP-utilizing enzyme family.
CC {ECO:0000256|ARBA:ARBA00007837}.
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DR EMBL; CP029357; AWK89624.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2S2CYN8; -.
DR KEGG; azz:DEW08_26860; -.
DR OrthoDB; 9765468at2; -.
DR Proteomes; UP000245629; Plasmid unnamed2.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008965; F:phosphoenolpyruvate-protein phosphotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:InterPro.
DR Gene3D; 2.70.70.10; Glucose Permease (Domain IIA); 1.
DR Gene3D; 3.30.1340.10; HPr-like; 1.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR Gene3D; 3.50.30.10; Phosphohistidine domain; 1.
DR Gene3D; 1.10.274.10; PtsI, HPr-binding domain; 1.
DR InterPro; IPR011055; Dup_hybrid_motif.
DR InterPro; IPR000032; HPr-like.
DR InterPro; IPR035895; HPr-like_sf.
DR InterPro; IPR008279; PEP-util_enz_mobile_dom.
DR InterPro; IPR000121; PEP_util_C.
DR InterPro; IPR023151; PEP_util_CS.
DR InterPro; IPR036637; Phosphohistidine_dom_sf.
DR InterPro; IPR006318; PTS_EI-like.
DR InterPro; IPR001127; PTS_EIIA_1_perm.
DR InterPro; IPR008731; PTS_EIN.
DR InterPro; IPR001020; PTS_HPr_His_P_site.
DR InterPro; IPR002114; PTS_HPr_Ser_P_site.
DR InterPro; IPR036618; PtsI_HPr-bd_sf.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR NCBIfam; TIGR00830; PTBA; 1.
DR NCBIfam; TIGR01003; PTS_HPr_family; 1.
DR NCBIfam; TIGR01417; PTS_I_fam; 1.
DR PANTHER; PTHR46244; PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE; 1.
DR PANTHER; PTHR46244:SF3; PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE; 1.
DR Pfam; PF05524; PEP-utilisers_N; 1.
DR Pfam; PF00391; PEP-utilizers; 1.
DR Pfam; PF02896; PEP-utilizers_C; 1.
DR Pfam; PF00381; PTS-HPr; 1.
DR Pfam; PF00358; PTS_EIIA_1; 1.
DR PRINTS; PR00107; PHOSPHOCPHPR.
DR PRINTS; PR01736; PHPHTRNFRASE.
DR SUPFAM; SSF51261; Duplicated hybrid motif; 1.
DR SUPFAM; SSF47831; Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain; 1.
DR SUPFAM; SSF55594; HPr-like; 1.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR SUPFAM; SSF52009; Phosphohistidine domain; 1.
DR PROSITE; PS00742; PEP_ENZYMES_2; 1.
DR PROSITE; PS51093; PTS_EIIA_TYPE_1; 1.
DR PROSITE; PS00371; PTS_EIIA_TYPE_1_HIS; 1.
DR PROSITE; PS51350; PTS_HPR_DOM; 1.
DR PROSITE; PS00369; PTS_HPR_HIS; 1.
DR PROSITE; PS00589; PTS_HPR_SER; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683};
KW Plasmid {ECO:0000313|EMBL:AWK89624.1};
KW Pyruvate {ECO:0000313|EMBL:AWK89624.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000245629};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:AWK89624.1};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT DOMAIN 22..126
FT /note="PTS EIIA type-1"
FT /evidence="ECO:0000259|PROSITE:PS51093"
FT DOMAIN 176..263
FT /note="HPr"
FT /evidence="ECO:0000259|PROSITE:PS51350"
SQ SEQUENCE 856 AA; 88448 MW; 902B2FE70B45A895 CRC64;
MSAITLAAPL AGWAMPLEDV PDPAFAQGLV GDGMAIDPTV NELRAPCDGV VLSVHRARHA
CTIRAASGAE VLLHLGVDTV GLNGEGFTAH VRDGQPVRTG DLLLSFDMDL VGSRAHSLVS
MMVVVNDGFT VDGRTVNREV AAGAPVMTVE GGTAPAAAAP AAAAGPAAGP GAGGETAERT
VRLRIAHGLH ARPAAALVAA AKAHPGAVTI LCRGQTANAK SVVALMGLGT VANDELTVRA
TGPGAPGVVE SLAGLIESGL GDEIVEGPPP AAATAAPAAA PAVAEEIGPP FAPGEEVLLK
GTIAVPGVAV GTALRRFRAA VTVREEGRGA AEEERRLRGA LDRVVAGLSD AARRMPEHAA
IFQAHQELLA DPELLDGALA DIGAGKSAEW AWSRAARGQA EVLSRLADPR MAERAADLRD
LEQQVLAALS GREPSVGLAG VPEGSIVLAD EILPSDLAGV PAGRLGGICM VHGGPTSHAV
ILAAALGVPT VVAVGRQAER IPDGASVIVD GNRGEIRVSP PAEAMEATRG AVASRAARRE
ENRRTAHEPC RTADGTRIEV LANLGRVSDA PGAVTEGAEG CGLLRTEFLF LERQSAPSED
EQYRQYQQIA DDLGGRPLVI RTLDVGGDKP LPYLPLPKEE NPVLGLRGVR VGLREPELLR
AQIRAILRVR PAGVCRIMVP MIASPAELRT VRAMVDEERA RLGRAEPIEV GAMIEVPAAA
LVADRMSIVA DFLSIGTNDL TQYVLAMDRG NPHVAAQLDA LHPGVLRLIR QTVAGAEVLG
RTVAVCGGAA SDPMAAPLLI GLGVTELSAT PAVIADLKAF IRTLRIADCR RIAEMALSLD
SAEDVRRLVT ETWPDL
//
