ID A0A2S4N8F5_9FLAO Unreviewed; 345 AA.
AC A0A2S4N8F5;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU362028};
DE EC=5.4.99.- {ECO:0000256|RuleBase:RU362028};
GN ORFNames=Q361_10630 {ECO:0000313|EMBL:POS01968.1};
OS Flavobacterium croceum DSM 17960.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=1121886 {ECO:0000313|EMBL:POS01968.1, ECO:0000313|Proteomes:UP000237056};
RN [1] {ECO:0000313|EMBL:POS01968.1, ECO:0000313|Proteomes:UP000237056}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17960 {ECO:0000313|EMBL:POS01968.1,
RC ECO:0000313|Proteomes:UP000237056};
RA Goeker M.;
RT "Genomic Encyclopedia of Type Strains, Phase I: the one thousand microbial
RT genomes (KMG-I) project.";
RL Submitted (JAN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil.
CC {ECO:0000256|RuleBase:RU362028}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC Evidence={ECO:0000256|RuleBase:RU362028};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000256|ARBA:ARBA00010876, ECO:0000256|RuleBase:RU362028}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:POS01968.1}.
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DR EMBL; PQNY01000006; POS01968.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2S4N8F5; -.
DR OrthoDB; 9807829at2; -.
DR Proteomes; UP000237056; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006224; PsdUridine_synth_RluA-like_CS.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR NCBIfam; TIGR00005; rluA_subfam; 1.
DR PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1.
DR PANTHER; PTHR21600:SF44; PSEUDOU_SYNTH_2 DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|RuleBase:RU362028};
KW Reference proteome {ECO:0000313|Proteomes:UP000237056};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT DOMAIN 29..88
FT /note="RNA-binding S4"
FT /evidence="ECO:0000259|SMART:SM00363"
FT ACT_SITE 152
FT /evidence="ECO:0000256|PIRSR:PIRSR606225-1"
SQ SEQUENCE 345 AA; 39477 MW; A7D2A5B4595B82BF CRC64;
MNSFIDTQDL EEELFEHHRF EVPKGQAMLR IDKYLMQVLP NTTRNKIQNA ASNGDVYVND
TPVKSNYKVK PFDIVKILLA HPPYENRIDP ENIPLEIVYE DDELIVLNKP AGLVVHPGHG
NYTGTLVNGL AFHFQNLPMN SSNRPGLVHR IDKNTSGLLV VAKTEYAMSF LAKQFEEKTT
EREYIALVWG NVKEDSGTIT GNIARHVKDR MQMAVFPDGD VGKHAVTHYK VLERLGYVTL
VSCKLETGRT HQIRVHMKYI GHTLFNDERY GGNLILKGTT FTKYKQFVEN CFKILPRQAL
HARTLGFLHP TTGAFLKFEC DIPNDMLECI DKWRNYAKNI TVDEE
//
