ID A0A2S4W126_9BASI Unreviewed; 698 AA.
AC A0A2S4W126;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=tRNA (guanine(26)-N(2))-dimethyltransferase {ECO:0000256|ARBA:ARBA00039099};
DE EC=2.1.1.216 {ECO:0000256|ARBA:ARBA00039099};
GN ORFNames=PSTT_02046 {ECO:0000313|EMBL:POW15451.1};
OS Puccinia striiformis.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Pucciniomycotina;
OC Pucciniomycetes; Pucciniales; Pucciniaceae; Puccinia.
OX NCBI_TaxID=27350 {ECO:0000313|EMBL:POW15451.1, ECO:0000313|Proteomes:UP000239156};
RN [1] {ECO:0000313|EMBL:POW15451.1, ECO:0000313|Proteomes:UP000239156}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=93-210 {ECO:0000313|EMBL:POW15451.1,
RC ECO:0000313|Proteomes:UP000239156};
RA Xia C.;
RT "Gene loss provides genomic basis for host adaptation in cereal stripe rust
RT fungi.";
RL Submitted (DEC-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. Trm1 family. {ECO:0000256|PROSITE-ProRule:PRU00958}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:POW15451.1}.
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DR EMBL; PKSL01000012; POW15451.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2S4W126; -.
DR VEuPathDB; FungiDB:PSHT_16339; -.
DR VEuPathDB; FungiDB:PSTT_02046; -.
DR Proteomes; UP000239156; Unassembled WGS sequence.
DR GO; GO:0160102; F:tRNA (guanine(10)-N2)-methyltransferase activity; IEA:InterPro.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.30.56.70; N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR002905; Trm1.
DR InterPro; IPR042296; tRNA_met_Trm1_C.
DR NCBIfam; TIGR00308; TRM1; 1.
DR PANTHER; PTHR10631; N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE; 1.
DR PANTHER; PTHR10631:SF3; TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE; 1.
DR Pfam; PF02005; TRM; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51626; SAM_MT_TRM1; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU00958}; Reference proteome {ECO:0000313|Proteomes:UP000239156};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU00958};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU00958};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU00958}; tRNA processing {ECO:0000256|PROSITE-ProRule:PRU00958};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|PROSITE-
KW ProRule:PRU00958}.
FT REGION 83..150
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 83..97
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 98..134
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 698 AA; 76442 MW; 1BFFB8A514DF4DD3 CRC64;
MNQIRRLFSI NRIMDNFPQP PAGYQAHQEA STQILIKITE ESAAQPSEAF INPVQEYNRD
LSISAIKAWS SIRNQELESK WIKSKNSRTK KRGPSDSNHE LINSNKIKQA ESDQPVTQDT
PVDVTDQSTQ QPTPPPAASE DPPNNTKNTY KFQPQKFSAL EALSATGLRA IRYAKEIPSL
KSILANDLSP SAVKLIKANI THNGLDPAEP ASQEGRVKVN QGDACDLMYS HCQPSHQFDV
VDLDPYGTAA PFIDAAVQCV RDGGLLCVTC TDLAVLAGSA YPEKCFSNYG GTSMRAEYSH
EYALRQVIHS LASSASRYGK QIEPLLSLSI DFYVRIFVRV SHSPAEVKKC ITNTAIVYVC
SGCSAFHFQS MGRANTKPTK AGRGTNVSYQ NALGPPMSSG SDCEECGGKF HVAGPLWSGP
LHDPNFCDKI LEVQADDTPL KTVERIRGMD GLAKTGYQEL PESPFFFTPA KLSGIFKCSS
PSLNTVGSAI LNGGYSISRS HCQPGSIKTD APRAFIHDLM KAWIDSQKTS TTTTTTTLEK
KDSLIADTKA DVISTTETSP QVNGHGQDNL GDGAKDEHVG EADEKVVEEV EVNQDDKVMD
VKTQPEKVEE ATTNNNQDPN IISAERASEI LEACRIHGAK HSSNPRLMSL LISRPPRYQI
DLKYNKLVEK ALLTSLKIVR YQSNPTPNWG PKARAIKK
//
