ID   A0A2S4YD58_9ACTN        Unreviewed;       594 AA.
AC   A0A2S4YD58;
DT   18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT   18-JUL-2018, sequence version 1.
DT   27-MAR-2024, entry version 14.
DE   RecName: Full=galacturonan 1,4-alpha-galacturonidase {ECO:0000256|ARBA:ARBA00038933};
DE            EC=3.2.1.67 {ECO:0000256|ARBA:ARBA00038933};
DE   AltName: Full=Galacturan 1,4-alpha-galacturonidase {ECO:0000256|ARBA:ARBA00043142};
DE   AltName: Full=Poly(1,4-alpha-D-galacturonide)galacturonohydrolase {ECO:0000256|ARBA:ARBA00041604};
GN   ORFNames=C3488_25405 {ECO:0000313|EMBL:POX46802.1};
OS   Streptomyces sp. Ru72.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=2080747 {ECO:0000313|EMBL:POX46802.1, ECO:0000313|Proteomes:UP000237426};
RN   [1] {ECO:0000313|EMBL:POX46802.1, ECO:0000313|Proteomes:UP000237426}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ru72 {ECO:0000313|EMBL:POX46802.1,
RC   ECO:0000313|Proteomes:UP000237426};
RA   Suneja G., Sharma R.;
RT   "Genome sequencing of cellulose degrading bacteria.";
RL   Submitted (FEB-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[(1->4)-alpha-D-galacturonosyl](n) + H2O = [(1->4)-alpha-D-
CC         galacturonosyl](n-1) + alpha-D-galacturonate; Xref=Rhea:RHEA:14117,
CC         Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14572, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:58658, ChEBI:CHEBI:140523; EC=3.2.1.67;
CC         Evidence={ECO:0000256|ARBA:ARBA00036499};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 28 family.
CC       {ECO:0000256|ARBA:ARBA00008834, ECO:0000256|RuleBase:RU361169}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:POX46802.1}.
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DR   EMBL; PQSR01000062; POX46802.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2S4YD58; -.
DR   OrthoDB; 9795222at2; -.
DR   Proteomes; UP000237426; Unassembled WGS sequence.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProt.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0004650; F:polygalacturonase activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   Gene3D; 2.160.20.10; Single-stranded right-handed beta-helix, Pectin lyase-like; 1.
DR   InterPro; IPR015919; Cadherin-like_sf.
DR   InterPro; IPR000743; Glyco_hydro_28.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR006626; PbH1.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   InterPro; IPR006311; TAT_signal.
DR   PANTHER; PTHR31736; -; 1.
DR   PANTHER; PTHR31736:SF14; EXOPOLYGALACTURONASE X-1-RELATED; 1.
DR   Pfam; PF00295; Glyco_hydro_28; 1.
DR   Pfam; PF05345; He_PIG; 1.
DR   SMART; SM00710; PbH1; 6.
DR   SUPFAM; SSF49313; Cadherin-like; 1.
DR   SUPFAM; SSF51126; Pectin lyase-like; 1.
DR   PROSITE; PS51318; TAT; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361169};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361169};
KW   Reference proteome {ECO:0000313|Proteomes:UP000237426};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..37
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           38..594
FT                   /note="galacturonan 1,4-alpha-galacturonidase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5015409915"
SQ   SEQUENCE   594 AA;  64038 MW;  D6BA8BE90EDDF24B CRC64;
     MNDTQGTGMT RRTVLQAAGA AAAAYSLVGA AAATARADDT PASTDKLVVY PIPSGVPTNL
     SYSVKARTPG GDWQTVPVYR ARAKQIDANT GSGPVFNSSV AMFDFQGTVE VAVTSAKGTI
     GTARIRPLSY GTQFTVDGAT IGFTLTEPRN LSIEVDGDIF NNLQLHANPI ETNVPDPDDE
     DVLYFGPGLH KTTNNVLTVP SGKTVYLAGG AVLTSQVAFQ NVENARLRGR GVLYNSPSGV
     LVEYSKNIEI DGVTVLNPSS GYAITVGQSQ QVTIHNFHAY SHGQWGDGID IFCSQDVLID
     NVWMRNADDC IAIYAHRWNY YGDCRNITVR NSTLWADVAH PINVGTHGNT DNPETLENLV
     FSNIDILDHR EPQMNYQGCI ALNPGDSNLV SNVRAQDIRV EDFRWGQLLN MRVMFNTTYN
     TSVGRGIDGV YIKNLSYNGT HANPAILVGY DADHAIKNVT FQNLVINGKF IGNGMRKPGW
     YTFWDMMPGY ANEHVINTRF LNSTDATSTN APQITSPDQA AGTKNQVFNY LITASELPTS
     FSAAGLPKGL DIDTGTGLIS GVMRDNVGSF DVTVSATNSV GTATRTVTLT VQHP
//