ID A0A2S5X6F0_9MICO Unreviewed; 851 AA.
AC A0A2S5X6F0;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=Aminopeptidase N {ECO:0000256|ARBA:ARBA00015611};
DE EC=3.4.11.2 {ECO:0000256|ARBA:ARBA00012564};
DE AltName: Full=Alanine aminopeptidase {ECO:0000256|ARBA:ARBA00029811};
DE AltName: Full=Lysyl aminopeptidase {ECO:0000256|ARBA:ARBA00031533};
GN Name=pepN {ECO:0000313|EMBL:PPG40059.1};
GN ORFNames=C5C17_09395 {ECO:0000313|EMBL:PPG40059.1};
OS Pseudoclavibacter sp. RFBA6.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Pseudoclavibacter.
OX NCBI_TaxID=2080573 {ECO:0000313|EMBL:PPG40059.1, ECO:0000313|Proteomes:UP000237697};
RN [1] {ECO:0000313|EMBL:PPG40059.1, ECO:0000313|Proteomes:UP000237697}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RFBA6 {ECO:0000313|EMBL:PPG40059.1,
RC ECO:0000313|Proteomes:UP000237697};
RA Davis E.W.II., Tabima J.F., Weisberg A.J., Lopes L.D., Wiseman M.S.,
RA Wiseman M.S., Pupko T., Belcher M.S., Sechler A.J., Tancos M.A.,
RA Schroeder B.K., Murray T.D., Luster D.G., Schneider W.L., Rogers E.,
RA Andreote F.D., Grunwald N.J., Putnam M.L., Chang J.H.;
RT "Bacteriophage NCPPB3778 and a type I-E CRISPR drive the evolution of the
RT US Biological Select Agent, Rathayibacter toxicus.";
RL Submitted (FEB-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa- from a
CC peptide, amide or arylamide. Xaa is preferably Ala, but may be most
CC amino acids including Pro (slow action). When a terminal hydrophobic
CC residue is followed by a prolyl residue, the two may be released as
CC an intact Xaa-Pro dipeptide.; EC=3.4.11.2;
CC Evidence={ECO:0000256|ARBA:ARBA00000098};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- SIMILARITY: Belongs to the peptidase M1 family.
CC {ECO:0000256|ARBA:ARBA00010136}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PPG40059.1}.
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DR EMBL; PSUA01000011; PPG40059.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2S5X6F0; -.
DR Proteomes; UP000237697; Unassembled WGS sequence.
DR GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09602; M1_APN; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR Gene3D; 2.60.40.1730; tricorn interacting facor f3 domain; 1.
DR InterPro; IPR045357; Aminopeptidase_N-like_N.
DR InterPro; IPR042097; Aminopeptidase_N-like_N_sf.
DR InterPro; IPR024571; ERAP1-like_C_dom.
DR InterPro; IPR012778; Pept_M1_aminopeptidase.
DR InterPro; IPR001930; Peptidase_M1.
DR InterPro; IPR014782; Peptidase_M1_dom.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR NCBIfam; TIGR02412; pepN_strep_liv; 1.
DR PANTHER; PTHR11533:SF304; AMINOPEPTIDASE N; 1.
DR PANTHER; PTHR11533; PROTEASE M1 ZINC METALLOPROTEASE; 1.
DR Pfam; PF11838; ERAP1_C; 1.
DR Pfam; PF01433; Peptidase_M1; 1.
DR Pfam; PF17900; Peptidase_M1_N; 1.
DR PRINTS; PR00756; ALADIPTASE.
DR SUPFAM; SSF63737; Leukotriene A4 hydrolase N-terminal domain; 1.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Aminopeptidase {ECO:0000313|EMBL:PPG40059.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000237697};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 22..190
FT /note="Aminopeptidase N-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF17900"
FT DOMAIN 235..446
FT /note="Peptidase M1 membrane alanine aminopeptidase"
FT /evidence="ECO:0000259|Pfam:PF01433"
FT DOMAIN 528..837
FT /note="ERAP1-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11838"
SQ SEQUENCE 851 AA; 94130 MW; C284E8EB111F4E07 CRC64;
MPGENLTRAE SVERRGVVLG VEHYDVALDL TTGPETFRSI TTVTFSAVPG ASTFIDAITR
QVHSVVLNGE ALDVAAVNDG VRIQLPNLAE RNTLTVDADA IYMNTGEGLH RFVDPADGEV
YLYSQFEVPD SRRMFAVFEQ PDLKANFTFH VTAPAAWQVV SNEPSQTPQP IDAERAVWHF
DKTPRISSYI TALVAGPYTV VRDELTSIDG RTIPLAVYCR ASLAPNLDAD NIFRTTKQGF
EYYEKAFGVP YPFTKYDQLF VPEFNAGAME NAGCVTFTET YVFRSKVADA VRERRVVTIL
HELAHMWFGD LVTMKWWNDL WLNESFAEFI STLATAEATE WTENWVTFNA SEKSWAYRQD
QLPSTHPIVA EIRDLDDVQV NFDGITYAKG GSVLKQLFAW VGRDAFFAGI NAYFSKHAWG
NTELRDLLAE LEVTSGRDLT EWSAKWLETA GVNTLRPVYE LDEHGNFTSF AIEQTATAEH
PTIRPHRLAI GLYNVQGDEL VRIHRFELDV DGERTEVPSF VGGALPDLII VNDDDLAYAK
IRLDAHSVTT VQQHLPKLRA PLSRSLVVSS LWDATRDAEL RARDFIRAVL GVIGTETEST
TRRTVLGQLI SAAKFYTAPE DRAEIVEVVT DELFELAKRA EAGSDAQFSF VKAFASLAET
GPQLDAVAAL FDGTFALEGL EIDTDLGWEL LYALVAGGRA GEAEIAARLE TDRTADGERS
AAHARAALPT PEGKEAAWAS VMERSDATNA IVRATVAGFD RVNDEALLEP FVDRYFGHIS
TIWADRSFQI AETLIEGFFP AGLATARIGD AADDWLANNE AAAPAFRRMI IEGRAGVVRA
LAAQERDAQP E
//
