ID   A0A2S8EPX2_9RHOB        Unreviewed;      1003 AA.
AC   A0A2S8EPX2;
DT   18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT   18-JUL-2018, sequence version 1.
DT   24-JAN-2024, entry version 18.
DE   SubName: Full=Sarcosine oxidase subunit alpha family protein {ECO:0000313|EMBL:PQO21892.1};
GN   ORFNames=C2I36_15895 {ECO:0000313|EMBL:PQO21892.1};
OS   Rhodobacteraceae bacterium WD3A24.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC   Paracoccaceae.
OX   NCBI_TaxID=2086263 {ECO:0000313|EMBL:PQO21892.1, ECO:0000313|Proteomes:UP000237945};
RN   [1] {ECO:0000313|EMBL:PQO21892.1, ECO:0000313|Proteomes:UP000237945}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WD3A24 {ECO:0000313|EMBL:PQO21892.1,
RC   ECO:0000313|Proteomes:UP000237945};
RA   Wang S.;
RT   "The draft genome sequence of Pararoseibaca halophilus WD3A24.";
RL   Submitted (JAN-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the GcvT family.
CC       {ECO:0000256|ARBA:ARBA00008609}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PQO21892.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; PUHN01000083; PQO21892.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2S8EPX2; -.
DR   OrthoDB; 5287468at2; -.
DR   Proteomes; UP000237945; Unassembled WGS sequence.
DR   GO; GO:0008115; F:sarcosine oxidase activity; IEA:InterPro.
DR   GO; GO:0046653; P:tetrahydrofolate metabolic process; IEA:InterPro.
DR   Gene3D; 3.10.20.440; 2Fe-2S iron-sulphur cluster binding domain, sarcosine oxidase, alpha subunit, N-terminal domain; 1.
DR   Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR   InterPro; IPR042204; 2Fe-2S-bd_N.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR023753; FAD/NAD-binding_dom.
DR   InterPro; IPR028896; GCST/YgfZ/DmdA.
DR   InterPro; IPR013977; GCV_T_C.
DR   InterPro; IPR006222; GCV_T_N.
DR   InterPro; IPR029043; GcvT/YgfZ_C.
DR   InterPro; IPR006277; Sarcosine_oxidase_asu.
DR   InterPro; IPR041117; SoxA_A3.
DR   InterPro; IPR027266; TrmE/GcvT_dom1.
DR   NCBIfam; TIGR01372; soxA; 1.
DR   PANTHER; PTHR43757; AMINOMETHYLTRANSFERASE; 1.
DR   PANTHER; PTHR43757:SF2; AMINOMETHYLTRANSFERASE, MITOCHONDRIAL; 1.
DR   Pfam; PF13510; Fer2_4; 1.
DR   Pfam; PF01571; GCV_T; 1.
DR   Pfam; PF08669; GCV_T_C; 1.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   Pfam; PF17806; SO_alpha_A3; 1.
DR   PIRSF; PIRSF037980; SoxA; 1.
DR   PRINTS; PR00368; FADPNR.
DR   PRINTS; PR00411; PNDRDTASEI.
DR   SUPFAM; SSF101790; Aminomethyltransferase beta-barrel domain; 1.
DR   SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR   SUPFAM; SSF103025; Folate-binding domain; 1.
PE   3: Inferred from homology;
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Reference proteome {ECO:0000313|Proteomes:UP000237945}.
FT   DOMAIN          170..422
FT                   /note="FAD/NAD(P)-binding"
FT                   /evidence="ECO:0000259|Pfam:PF07992"
FT   DOMAIN          522..604
FT                   /note="SoxA A3"
FT                   /evidence="ECO:0000259|Pfam:PF17806"
FT   DOMAIN          619..884
FT                   /note="Aminomethyltransferase folate-binding"
FT                   /evidence="ECO:0000259|Pfam:PF01571"
FT   DOMAIN          910..995
FT                   /note="Glycine cleavage T-protein C-terminal barrel"
FT                   /evidence="ECO:0000259|Pfam:PF08669"
FT   REGION          923..946
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1003 AA;  109299 MW;  21FBC279EEA133D3 CRC64;
     MSTRLATGGR LIDRSARRRF TFDGRAHEGY AGDTLASALL AGGHTLLGRS FKYHRPRGLV
     ASGAEEPNAL VGLGAGARFE PNQRATTTEL FDGLQAVSQN AWPSLDWDVG VVNNAFARFL
     PAGFYYKMFM HPRPLWKHLF EPFIRRSAGL GRAPSERDAD RYEHFYAFCD VLVVGGGIAG
     LQAALAAGRA GARVILLEQT AHWGGRSPVD GVRIDGGPAE AWINAAVQEL DGMENVTLRP
     RTMGAGVYDH GYVLGYERVG DHDPAAEVPR HRLWRIRAAR LITATGALER PLSFAGNDVP
     GVMLASAVRD YVVNWAVSPG DRTVVVTNND DAYRTALALK SAGLEVPAIL DARPRAEGEL
     PEMARAHGIR VETGKGVAKV RGGRHVTAVG VCAQAGEGAV LEEIACDAVA MSGGWSPVVH
     LWSHCGGKLE WDAAQALFRP DPDRAPTGHD GHPFVATAGA ASGALQADRA MADADENMKN
     VLQGIGRKSP SRKPPKVESA EEAPLAPVWQ MPQGAGPALR AKMWLDYQND VKVSDVRLAA
     QEGYESVEHT KRYTTLGMAT DQGKLSNING LATLSQALHE EIPQVGTTTF RPPYTPISMA
     AIAGEARGPV FQPLRRTPMH EWHEANGADW EPVGQWRRPY CYRRAGESRR DAVHREVRNT
     RNALGLLDAS TLGKIMVSGP DAERFIDMLY TGKMSTLKPG KCRYGLMCDE NGFLMDDGVC
     ARLDERSFLC HTTSGGAERI HGWMEDWLQC EWWDWQVHVV NLTEQFAQVA VVGPNARNLL
     ERLGGIDVSK ETLPFMAWTE GTLGGFRARV FRISFSGELS FEIAVPASEG RAFWERLVEA
     GEGFGAMPYG TEALHVMRAE KGFIMIGDET DGTIIPQDLG LDWAISKKKE DFLGKRGMER
     VNMTSPDRWK LVGLETLDGS VLPDGAHAPA EGLNANGQRN TQGRVTSTYY SPTLGRGIAM
     ALVHRGPERM GETIAFNVPG RTPVKAKIVE PVFYDPKGER QNV
//