ID A0A2S9A9K3_9MICO Unreviewed; 803 AA.
AC A0A2S9A9K3;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=CQ047_08250 {ECO:0000313|EMBL:PRB10176.1};
OS Microbacterium sp. MYb72.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Microbacterium.
OX NCBI_TaxID=1848693 {ECO:0000313|EMBL:PRB10176.1, ECO:0000313|Proteomes:UP000238041};
RN [1] {ECO:0000313|EMBL:PRB10176.1, ECO:0000313|Proteomes:UP000238041}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MYb72 {ECO:0000313|EMBL:PRB10176.1,
RC ECO:0000313|Proteomes:UP000238041};
RA Zimmermann J., Obeng N., Yang W., Obeng O., Kissoyan K., Pees B.,
RA Dirksen P., Hoppner M., Franke A., Rosenstiel P., Leippe M., Dierking K.,
RA Kaleta C., Schulenburg H.;
RT "Genomic, metabolic, and phenotypic characteristics of bacterial isolates
RT from the natural microbiome of the model nematode Caenorhabditis elegans.";
RL Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SIMILARITY: Belongs to the DnaX/STICHEL family.
CC {ECO:0000256|ARBA:ARBA00006360}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PRB10176.1}.
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DR EMBL; PCOT01000011; PRB10176.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2S9A9K3; -.
DR OrthoDB; 9810148at2; -.
DR Proteomes; UP000238041; Unassembled WGS sequence.
DR GO; GO:0009360; C:DNA polymerase III complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR CDD; cd00009; AAA; 1.
DR CDD; cd18137; HLD_clamp_pol_III_gamma_tau; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 1.20.272.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR008921; DNA_pol3_clamp-load_cplx_C.
DR InterPro; IPR022754; DNA_pol_III_gamma-3.
DR InterPro; IPR012763; DNA_pol_III_sug/sutau_N.
DR InterPro; IPR045085; HLD_clamp_pol_III_gamma_tau.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR02397; dnaX_nterm; 1.
DR PANTHER; PTHR11669:SF0; PROTEIN STICHEL; 1.
DR PANTHER; PTHR11669; REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT; 1.
DR Pfam; PF13177; DNA_pol3_delta2; 1.
DR Pfam; PF12169; DNA_pol3_gamma3; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF48019; post-AAA+ oligomerization domain-like; 1.
PE 3: Inferred from homology;
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022932};
KW Transferase {ECO:0000256|ARBA:ARBA00022932}.
FT DOMAIN 37..181
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT REGION 395..488
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 573..775
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 466..480
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 677..703
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 708..723
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 803 AA; 81560 MW; 1183EAAE84A3F4A2 CRC64;
MVTTALYRRY RPESFGEMIG QSQVTDPLMT ALRGDRVGHA YLFSGPRGCG KTTSARILAR
CLNCAEGPTD VPCGKCPSCI ELSRAGGGSL DVVEIDAASH NGVDDARDLR ERATFAPSRD
RYKIFILDEA HMVTPQGFNA LLKLVEEPPE HVKFIFATTE PEKVLGTIRS RTHHYPFRLV
PPAAMLEYVE KLCTEEGVVV EQGVLPLVVR AGGGSPRDTL SLLDQLIAGS DSPAGSETVT
VAYERAVALL GYTHATVLDE IVDALAAGDA AAAFPAIDKV VQTGQDPRRF VDDLLERLRD
LIVIAAVGAG ASAVLRGIAA DELDRMREQA ASFGSARLSR TADIVSAALD DMSGATSPRL
HLELLVARVL AGGGADAAAP APVSAAARPA AAPAPASAAP APAASAPASA APAPATPASA
PAAPAPAAPA AQTPAVQPSS APVVAPRGSG AGEAERGEIS VDSAAQPAPD SPSTSEAPAA
ASAPAPSGPV TFERISGSWS AVLKRLEDIS RTSWLLATAV QPLAFDTASE VLTLGFTSQH
DVQKFKGTVP GSGPSDHLRT AIEQELGVTV KYRPAPMPAG GAPRQPAAAS SPSSPSGDAP
ASEPASAGSS RPQVRSDSAG PVTEWAVASI PSAGGQGAAS GGGAETPIPA ASTSAAPVPV
TAASVHAAPT PATPSPSAPG STASAAPSAP ATSVSSSPTA ASPMSVDGPP LPDEPPYYDD
EPPYDPAYEP AMSSAPPRRP EQSASAPTAA SAAPEPRRSA PAPVVTERTP AGGVQRYGEA
VIRQVLGATF VREEPYEPPT RFS
//
