ID A0A2S9I4P2_9GAMM Unreviewed; 1160 AA.
AC A0A2S9I4P2;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=CQW29_24995 {ECO:0000313|EMBL:PRD12766.1};
OS Pantoea coffeiphila.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Pantoea.
OX NCBI_TaxID=1465635 {ECO:0000313|EMBL:PRD12766.1, ECO:0000313|Proteomes:UP000239181};
RN [1] {ECO:0000313|EMBL:PRD12766.1, ECO:0000313|Proteomes:UP000239181}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=342 {ECO:0000313|EMBL:PRD12766.1,
RC ECO:0000313|Proteomes:UP000239181};
RA Siqueira K.A., Liotti R.G., Mendes T.A., Soares M.A.;
RT "Draft genome of two endophytic bacteria isolated from 'guarana' Paullinia
RT cupana (Mart.) Ducke.";
RL Submitted (OCT-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PRD12766.1}.
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DR EMBL; PDET01000027; PRD12766.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2S9I4P2; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000239181; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd07433; PHP_PolIIIA_DnaE1; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR048472; DNA_pol_IIIA_C.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR049821; PolIIIA_DnaE1_PHP.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF20914; DNA_pol_IIIA_C; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 7..74
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1160 AA; 130207 MW; 92602234E1DD4551 CRC64;
MAEPRFIHLR VHSDYSMIDG LAKTGPLVKN AAKMGMPAIG ITDFTNLCGL VKFYGTAHGQ
GMKPIIGADF NVASPLMGDE LSQLTVLAAD NEGYQNLTLL ISRAYQRGYG PAGPTIDRDW
LMEHQQGIIL LSGGRRGDVG KMLLRGNPTG VAECLSFYQT YFPDRYYLEL IRTGRQDEEV
YLHAAVDLAI AEGIPVVATN EVCFLQEDDF DAHEIRVAIH DGFTLDDPKR PKNYSPQQYM
RTEEEMCELF SDIPEALENS VEIARRCNVT IRLGEYFLPQ FPTGEMTTED FLVMKSKEGL
EERLEFLFPD EQLRAERRPE YDERLDVELN VINQMGFPGY FLIVMEFIQW SKDNDVPVGP
GRGSGAGSLV AYALKITDLD PLEFDLLFER FLNPERVSMP DFDVDFCMEK RDLVIDHVAE
MYGREAVSQI ITFGTMAAKA VIRDVGRVLG HPYGFVDRIS KLVPPDPGMT LEKAFAAEPQ
LPEIYEADEE VKALIDMARK LEGVTRNAGK HAGGVVIAPT KITDFAPLYC DENGNHPVTQ
YDKNDVEYAG LVKFDFLGLR TLTIIDWALG MINARRKKEG LEPIDIAAIP LEDQKSFDML
QRSETTAVFQ LESRGMKDLI KRLKPDCFED MIALVALFRP GPLQSGMVDN FIDRKHGREE
ISYPDIQWQH ESLKPVLEPT YGIILYQEQV MQIAQVLAGY SLGGADMLRR AMGKKNPVEM
AKQRGGFEDG AKARGVDGEL AIKIFDLVEK FAGYGFNKSH SAAYALVSYQ TLWLKAHYPA
EFMAAVMTAD MDNTEKVVGL VDECWRMGLK VLPPDINSGL YQFHVNDDGE IVYGIGAIKG
VGEGPIEAII EARNEGGYFR ELFDLCARTD TKKLNKRVLE KLIMSGAFDR LGPHRAALMS
ALPDALKAAD QHAKAEAIGQ ADMFGVLAEA PEQVEKSYSD VTPWPEQIQL DGERETLGLY
LTGHPINQYL KEIERYVGGL RLKDMHPTER GKMTTAAGLV VAARVMVTKR GNRIGICTLD
DRSGRLEVML FTDALDKFQH MLEKDRILIV SGQVSFDDFN GGLKMMARDM MDIDEAREKY
ARGLAISLTD RQIDDQLLNR LRQSLEPHRS GTIPVHLYYQ REDARAKLRF GATWRVSPSD
RLLNDLRSLI GSEQVELEFD
//
