ID   A0A2S9KF53_9BURK        Unreviewed;       283 AA.
AC   A0A2S9KF53;
DT   18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT   18-JUL-2018, sequence version 1.
DT   27-MAR-2024, entry version 35.
DE   SubName: Full=2,5-diketo-D-gluconic acid reductase {ECO:0000313|EMBL:PRD69032.1};
GN   ORFNames=C6P61_08095 {ECO:0000313|EMBL:PRD69032.1};
OS   Malikia spinosa.
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Malikia.
OX   NCBI_TaxID=86180 {ECO:0000313|EMBL:PRD69032.1, ECO:0000313|Proteomes:UP000238326};
RN   [1] {ECO:0000313|EMBL:PRD69032.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=83 {ECO:0000313|EMBL:PRD69032.1};
RA   Suzuki S., Ishii S., Walworth N., Bird L., Kuenen J.G., Nealson K.H.;
RT   "Comparative genomics illustrates the genes involved in a hyperalkaliphilic
RT   mechanisms of Serpentinomonas isolated from highly-alkaline calcium-rich
RT   serpentinized springs.";
RL   Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PRD69032.1}.
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DR   EMBL; PVLR01000020; PRD69032.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2S9KF53; -.
DR   OrthoDB; 9772407at2; -.
DR   Proteomes; UP000238326; Unassembled WGS sequence.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0071704; P:organic substance metabolic process; IEA:UniProt.
DR   GO; GO:0044281; P:small molecule metabolic process; IEA:UniProt.
DR   CDD; cd19133; AKR_AKR5F1; 1.
DR   Gene3D; 3.20.20.100; NADP-dependent oxidoreductase domain; 1.
DR   InterPro; IPR020471; AKR.
DR   InterPro; IPR018170; Aldo/ket_reductase_CS.
DR   InterPro; IPR023210; NADP_OxRdtase_dom.
DR   InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR   PANTHER; PTHR43827; 2,5-DIKETO-D-GLUCONIC ACID REDUCTASE; 1.
DR   PANTHER; PTHR43827:SF3; ALDO-KETO REDUCTASE; 1.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   PIRSF; PIRSF000097; AKR; 2.
DR   PRINTS; PR00069; ALDKETRDTASE.
DR   SUPFAM; SSF51430; NAD(P)-linked oxidoreductase; 1.
DR   PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
PE   4: Predicted;
FT   DOMAIN          24..257
FT                   /note="NADP-dependent oxidoreductase"
FT                   /evidence="ECO:0000259|Pfam:PF00248"
FT   ACT_SITE        49
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000097-1"
FT   BINDING         107
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000097-2"
FT   SITE            74
FT                   /note="Lowers pKa of active site Tyr"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000097-3"
SQ   SEQUENCE   283 AA;  31914 MW;  6623E5A74991BF4A CRC64;
     MDQVTLNNGV RMPIAGFGVF QIPDPAECER SVVDAIDAGY RLIDTAASYL NEAAVGRGLR
     ASGVPRDELF VTSKLWVQHA GYERTQQAID ASLRRLQLDY LDLYLIHQPY GDVHGSWRAM
     EEAYQAGKLR AIGVSNFHSD RLMDLMAFNT VPPAVNQIEV NPFHQQAESV AFMREHGVQA
     EAWAPFAEGR NQLFENAVLL EIGRKHGKSV GQVVLRWVVQ RDIVVLAKSV RKERMTENLA
     IFDFALDEDD MARIATLETG QSRFFSHRDP AIVKWMSERT LDI
//