ID A0A2S9KIT9_9BURK Unreviewed; 220 AA.
AC A0A2S9KIT9;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE RecName: Full=Urease accessory protein UreG {ECO:0000256|HAMAP-Rule:MF_01389};
GN Name=ureG {ECO:0000256|HAMAP-Rule:MF_01389,
GN ECO:0000313|EMBL:PRD70368.1};
GN ORFNames=C6P61_01495 {ECO:0000313|EMBL:PRD70368.1}, F5985_16305
GN {ECO:0000313|EMBL:MYZ53646.1};
OS Malikia spinosa.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Malikia.
OX NCBI_TaxID=86180 {ECO:0000313|EMBL:PRD70368.1, ECO:0000313|Proteomes:UP000238326};
RN [1] {ECO:0000313|EMBL:PRD70368.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=83 {ECO:0000313|EMBL:PRD70368.1};
RA Suzuki S., Ishii S., Walworth N., Bird L., Kuenen J.G., Nealson K.H.;
RT "Comparative genomics illustrates the genes involved in a hyperalkaliphilic
RT mechanisms of Serpentinomonas isolated from highly-alkaline calcium-rich
RT serpentinized springs.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:MYZ53646.1, ECO:0000313|Proteomes:UP000481947}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AB6 {ECO:0000313|EMBL:MYZ53646.1,
RC ECO:0000313|Proteomes:UP000481947};
RA Tancsics A., Revesz F., Kriszt B.;
RT "Identification of Malikia spinosa a prominent benzene-, toluene-, and
RT ethylbenzene-degrading bacterium: enrichment, isolation and whole genome
RT sequencing.";
RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Facilitates the functional incorporation of the urease nickel
CC metallocenter. This process requires GTP hydrolysis, probably
CC effectuated by UreG. {ECO:0000256|HAMAP-Rule:MF_01389}.
CC -!- SUBUNIT: Homodimer. UreD, UreF and UreG form a complex that acts as a
CC GTP-hydrolysis-dependent molecular chaperone, activating the urease
CC apoprotein by helping to assemble the nickel containing metallocenter
CC of UreC. The UreE protein probably delivers the nickel.
CC {ECO:0000256|HAMAP-Rule:MF_01389}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01389}.
CC -!- SIMILARITY: Belongs to the SIMIBI class G3E GTPase family. UreG
CC subfamily. {ECO:0000256|ARBA:ARBA00005732, ECO:0000256|HAMAP-
CC Rule:MF_01389}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PRD70368.1}.
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DR EMBL; VYSB01000024; MYZ53646.1; -; Genomic_DNA.
DR EMBL; PVLR01000005; PRD70368.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2S9KIT9; -.
DR OrthoDB; 9802035at2; -.
DR Proteomes; UP000238326; Unassembled WGS sequence.
DR Proteomes; UP000481947; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR CDD; cd05540; UreG; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_01389; UreG; 1.
DR InterPro; IPR003495; CobW/HypB/UreG_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004400; UreG.
DR NCBIfam; TIGR00101; ureG; 1.
DR PANTHER; PTHR31715; UREASE ACCESSORY PROTEIN G; 1.
DR PANTHER; PTHR31715:SF0; UREASE ACCESSORY PROTEIN G; 1.
DR Pfam; PF02492; cobW; 1.
DR PIRSF; PIRSF005624; Ni-bind_GTPase; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW Chaperone {ECO:0000256|ARBA:ARBA00023186, ECO:0000256|HAMAP-Rule:MF_01389};
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01389};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134, ECO:0000256|HAMAP-
KW Rule:MF_01389};
KW Nickel insertion {ECO:0000256|ARBA:ARBA00022988, ECO:0000256|HAMAP-
KW Rule:MF_01389};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_01389}.
FT DOMAIN 21..188
FT /note="CobW/HypB/UreG nucleotide-binding"
FT /evidence="ECO:0000259|Pfam:PF02492"
FT BINDING 25..32
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01389"
SQ SEQUENCE 220 AA; 24033 MW; 5C778C5C9FC0CC27 CRC64;
MNAPSLHQIP GRTKILPPLR VGIGGPVGSG KTTLLEMLCK AMRARYDLIA ITNDIYTKED
QRLLTLSGAL PAERIMGVET GGCPHTAIRE DASINLEAID RMLEQFPDAD IVFIESGGDN
LAATFSPELS DLTIYVIDVA AGEKIPRKGG PGITKSDLFV INKTDLAPHV GADLEVMRRD
TARMRPDHER RPWFMTNLKT LDGLADVVGF IERRGLLSQS
//
