ID A0A2T0N3G7_9ACTN Unreviewed; 630 AA.
AC A0A2T0N3G7;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 24-JAN-2024, entry version 14.
DE RecName: Full=Beta-galactosidase {ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|RuleBase:RU000675};
GN ORFNames=B0I32_105169 {ECO:0000313|EMBL:PRX66729.1};
OS Nonomuraea fuscirosea.
OC Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
OC Streptosporangiaceae; Nonomuraea.
OX NCBI_TaxID=1291556 {ECO:0000313|EMBL:PRX66729.1, ECO:0000313|Proteomes:UP000238312};
RN [1] {ECO:0000313|EMBL:PRX66729.1, ECO:0000313|Proteomes:UP000238312}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGMCC 4.7104 {ECO:0000313|EMBL:PRX66729.1,
RC ECO:0000313|Proteomes:UP000238312};
RA Whitman W.;
RT "Genomic Encyclopedia of Type Strains, Phase III (KMG-III): the genomes of
RT soil and plant-associated and newly described type strains.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|RuleBase:RU000675};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PRX66729.1}.
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DR EMBL; PVNG01000005; PRX66729.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2T0N3G7; -.
DR OrthoDB; 9813184at2; -.
DR Proteomes; UP000238312; Unassembled WGS sequence.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR026283; B-gal_1-like.
DR InterPro; IPR048912; BetaGal1-like_ABD1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421:SF165; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21317; BetaGal_ABD_1; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PIRSF; PIRSF006336; B-gal; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW Reference proteome {ECO:0000313|Proteomes:UP000238312}.
FT DOMAIN 26..336
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 382..490
FT /note="Beta-galactosidase 1-like first all-beta"
FT /evidence="ECO:0000259|Pfam:PF21317"
FT DOMAIN 548..597
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
FT ACT_SITE 172
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT ACT_SITE 248
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
SQ SEQUENCE 630 AA; 67441 MW; E85BB6493AE41698 CRC64;
MKAGVKAGGP AGSATSALTW RGRRLHRHGV PHRILSGGLH YFRVHPDLWR DRIRRLADLG
LNTIDTYVAW NFHQPREDAA PRFDGWRDVE RFIRLVGEEG LDVVVRPGPY ICAEWSNGGL
PSWLTARDVA LRSSDPAFTA AVGRWFDELI PRLAALQAGA GGPVVAVQIE NEFGSYGDDQ
AYLRWNREAL TSRGIGELLF TADGPTELML DGGTLPGTLA AVTLGSKPAA ARRLQAARRP
DEPFVVAEFW NGWFDHWGER HHVRGVDSAV RTLEGIIADG GSVSVYMAHG GTNFGLWAGA
NEHEGRLQPT VTSYDSDAPV AEDGSLTPKF HAMRAALGAR GPVRVSERPP VLPPARVPLV
RRAGLLPGLR AVSAPATVEP RPASFERLGL DAGMVLHTAH PRVPEGEHRL TLIDVRDRAM
VYCDGTFLGV AAPGLPELSV RGTGAVVRLE ILVESLGRVN YGPGLGRHKG LLGPVLIDRR
IVQGWESTPI ALQEWTPADL ATATAAGEAA AEDLAAGDST AGDSAAAGPA AGELAPAGGG
FAEAIVRVGT PADTFLALPG SGRGLVWVNG FLLGRYWDIG PQVTLYCPAP LLREGDNVVT
VLELERPGAV LELRDRPELG PPEEYVEEFD
//
