ID A0A2T0QEY8_9ACTN Unreviewed; 407 AA.
AC A0A2T0QEY8;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000256|HAMAP-Rule:MF_01074};
DE Short=P2TMN nickel insertion protein {ECO:0000256|HAMAP-Rule:MF_01074};
DE EC=4.99.1.12 {ECO:0000256|HAMAP-Rule:MF_01074};
DE AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000256|HAMAP-Rule:MF_01074};
GN Name=larC {ECO:0000256|HAMAP-Rule:MF_01074};
GN ORFNames=CLV72_1011015 {ECO:0000313|EMBL:PRY02413.1};
OS Allonocardiopsis opalescens.
OC Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
OC Allonocardiopsis.
OX NCBI_TaxID=1144618 {ECO:0000313|EMBL:PRY02413.1, ECO:0000313|Proteomes:UP000237846};
RN [1] {ECO:0000313|EMBL:PRY02413.1, ECO:0000313|Proteomes:UP000237846}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 45601 {ECO:0000313|EMBL:PRY02413.1,
RC ECO:0000313|Proteomes:UP000237846};
RA Goeker M.;
RT "Genomic Encyclopedia of Archaeal and Bacterial Type Strains, Phase II
RT (KMG-II): from individual species to whole genera.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC (P2TMN), to form the mature cofactor. Is thus probably required for the
CC activation of nickel-pincer cofactor-dependent enzymes.
CC {ECO:0000256|HAMAP-Rule:MF_01074}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_01074};
CC -!- SIMILARITY: Belongs to the LarC family. {ECO:0000256|HAMAP-
CC Rule:MF_01074}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PRY02413.1}.
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DR EMBL; PVZC01000001; PRY02413.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2T0QEY8; -.
DR OrthoDB; 9765625at2; -.
DR Proteomes; UP000237846; Unassembled WGS sequence.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.70.1380; Transcriptional regulatory protein pf0864 domain like; 1.
DR HAMAP; MF_01074; LarC; 1.
DR InterPro; IPR002822; Ni_insertion.
DR NCBIfam; TIGR00299; nickel pincer cofactor biosynthesis protein LarC; 1.
DR PANTHER; PTHR36566; NICKEL INSERTION PROTEIN-RELATED; 1.
DR PANTHER; PTHR36566:SF1; NICKEL INSERTION PROTEIN-RELATED; 1.
DR Pfam; PF01969; Ni_insertion; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|HAMAP-Rule:MF_01074};
KW Nickel {ECO:0000256|ARBA:ARBA00022596, ECO:0000256|HAMAP-Rule:MF_01074};
KW Reference proteome {ECO:0000313|Proteomes:UP000237846}.
FT REGION 384..407
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 407 AA; 41481 MW; D1E1E0AFDDF15F8B CRC64;
MILFLHPFAG ISGDMLLGAL IDLGAPVAGI EAAVRSTGLD GWRLTAVRAA KGGLMATRAQ
VRVDDDATAR PAARLLEHVA RAEPEPVARL AATAVRLLAE AEGALHGVDP GEVHLHEIGG
LDTVVDTVGT AAALHLLGVE QVVSAPVVLG RGTVATAHGR LPAPAPATLA LLRGAEVSWA
AIAAETVTPT GAALLAAAGA SYRPAPPMTV RATGYGAGSR ELPDRPNVLQ ATLGEALGAG
AAGSGAYTVL ETNVDDTTGE VLGHLIGAAL DAGAADAWIT PAVMKKNRPA HTVHVLCRAS
DAPALETLVL AHTGSLGLRR TAVDRVAVER AGTTVHVSGH PVRIKYGPWQ AKPEHDDVVR
AAAALGVPPR TVAEEARRLA PSALGGDWPV ARPGRTDRCP PAREDAT
//
