ID A0A2T0RYJ1_9RHOB Unreviewed; 930 AA.
AC A0A2T0RYJ1;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE SubName: Full=Aconitase {ECO:0000313|EMBL:PRY26202.1};
GN ORFNames=CLV78_101297 {ECO:0000313|EMBL:PRY26202.1};
OS Aliiruegeria haliotis.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Roseobacteraceae; Aliiruegeria.
OX NCBI_TaxID=1280846 {ECO:0000313|EMBL:PRY26202.1, ECO:0000313|Proteomes:UP000239480};
RN [1] {ECO:0000313|EMBL:PRY26202.1, ECO:0000313|Proteomes:UP000239480}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 29328 {ECO:0000313|EMBL:PRY26202.1,
RC ECO:0000313|Proteomes:UP000239480};
RA Goeker M.;
RT "Genomic Encyclopedia of Archaeal and Bacterial Type Strains, Phase II
RT (KMG-II): from individual species to whole genera.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = 2-methyl-cis-
CC aconitate + H2O; Xref=Rhea:RHEA:17941, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:57429, ChEBI:CHEBI:57872; EC=4.2.1.99;
CC Evidence={ECO:0000256|ARBA:ARBA00000118};
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PRY26202.1}.
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DR EMBL; PVTD01000001; PRY26202.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2T0RYJ1; -.
DR OrthoDB; 9758061at2; -.
DR Proteomes; UP000239480; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 3.40.1060.10; Aconitase, Domain 2; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR Gene3D; 1.25.40.310; Aconitate B, HEAT-like domain; 1.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR015933; Aconitase_B_HEAT-like_dom.
DR InterPro; IPR036288; Aconitase_B_HEAT-like_dom_sf.
DR InterPro; IPR015929; Aconitase_B_swivel.
DR InterPro; IPR015932; Aconitase_dom2.
DR PANTHER; PTHR43160; ACONITATE HYDRATASE B; 1.
DR PANTHER; PTHR43160:SF4; ACONITATE HYDRATASE B; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF06434; Aconitase_2_N; 1.
DR Pfam; PF11791; Aconitase_B_N; 1.
DR SUPFAM; SSF74778; Aconitase B, N-terminal domain; 1.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000239480};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884}.
FT DOMAIN 6..162
FT /note="Aconitase B HEAT-like"
FT /evidence="ECO:0000259|Pfam:PF11791"
FT DOMAIN 176..407
FT /note="Aconitase B swivel"
FT /evidence="ECO:0000259|Pfam:PF06434"
FT DOMAIN 411..895
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
SQ SEQUENCE 930 AA; 100686 MW; B66321A4AB406615 CRC64;
MSLYTDYLDE IGTRKAQGLS PKPIDDGALV EELVAQIKDA DSVYRADSLN FFIYNTLPGT
TSAAGVKARF LKEIILGEAV VEEITPDFAF ELLSHMKGGP SVEVLLDLAL GEDAAIAAQA
ADVLKTQVFL YEADTDRLEA AYTAGNAVAK DILESYAKAE FFTKLPDIDE EIKVVTYIAA
EGDISTDLLS PGNQAHSRSD RELHGKCMIS EKAQQEIEAL KLQHPDKRVM LIAEKGTMGV
GSSRMSGVNN VALWTGKQAS PYVPFVNIAP VVAGTNGISP IFLTTVGVTG GIGLDLKNWV
KKVDADGKPI LNNDGNPILE QKYSVETGTV LTINTRSKKL YSEDGSEELT DVSSAFTPQK
VEFMKAGGSY AIVFGKKLQN FAAETLGVEP TPVFAPSREI SHEGQGLTAV EKIFNKNAVG
VSDGKVLHAG SDVRVKVNIV GSQDTTGLMT SQELEAMAAT VISPTVDGAY QSGCHTASVW
DKKAQANIPR LMKFMNNFGL ITARDPQGVY HSMTDVIHKV LNDITVDDWD IIIGGDSHTR
MSKGVAFGAD SGTVALALAT GEATMPIPES VKVTFKGAMK DHMDFRDVVH ATQAQMLKQF
GDNVFQGRVI EVHIGTLLAD QAFTFTDWTA EMKAKASICI SQDETLIGSL ELAKSRIKVM
IDKGMDNEAQ TLQGLIDKAD KRIAEIRSGE KPALTPDDNA KYFAEVVVDL DEIDEPMIAD
PDVNNADVSK RYTHDTIRPV SYYGGDKKVD LGFVGSCMVH KGDMKIVAQM LKNLEKAQGK
VEFNAPLVVA APTYNIIDEL KAEGDWEILQ KYSGFEFDDL MPKSAARTEY ENILYLERPG
CNLCMGNQEK AAKGDTVLAT STRLFQGRVV EDSEEKKGES LLASTPVVVL SAILGRTPSV
EEYKSAVEGI DLTKFAPPLE KPAGSRSAHF
//
