ID A0A2T0SXG3_9PSEU Unreviewed; 1455 AA.
AC A0A2T0SXG3;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=DNA gyrase subunit A {ECO:0000256|ARBA:ARBA00026190};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
GN ORFNames=CLV43_109263 {ECO:0000313|EMBL:PRY38043.1};
OS Umezawaea tangerina.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Umezawaea.
OX NCBI_TaxID=84725 {ECO:0000313|EMBL:PRY38043.1, ECO:0000313|Proteomes:UP000239494};
RN [1] {ECO:0000313|EMBL:PRY38043.1, ECO:0000313|Proteomes:UP000239494}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44720 {ECO:0000313|EMBL:PRY38043.1,
RC ECO:0000313|Proteomes:UP000239494};
RA Goeker M.;
RT "Genomic Encyclopedia of Archaeal and Bacterial Type Strains, Phase II
RT (KMG-II): from individual species to whole genera.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit
CC family. {ECO:0000256|ARBA:ARBA00008263}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PRY38043.1}.
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DR EMBL; PVTF01000009; PRY38043.1; -; Genomic_DNA.
DR OrthoDB; 9806486at2; -.
DR Proteomes; UP000239494; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0034335; F:DNA negative supercoiling activity; IEA:UniProt.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR CDD; cd00081; Hint; 1.
DR CDD; cd00187; TOP4c; 1.
DR Gene3D; 3.30.1360.40; -; 1.
DR Gene3D; 2.120.10.90; DNA gyrase/topoisomerase IV, subunit A, C-terminal; 1.
DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 3.
DR Gene3D; 3.10.28.10; Homing endonucleases; 1.
DR Gene3D; 3.90.199.10; Topoisomerase II, domain 5; 3.
DR Gene3D; 1.10.268.10; Topoisomerase, domain 3; 1.
DR InterPro; IPR006691; GyrA/parC_rep.
DR InterPro; IPR035516; Gyrase/topoIV_suA_C.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR013758; Topo_IIA_A/C_ab.
DR InterPro; IPR013757; Topo_IIA_A_a_sf.
DR InterPro; IPR002205; Topo_IIA_dom_A.
DR NCBIfam; TIGR01445; intein_Nterm; 1.
DR PANTHER; PTHR43493:SF5; DNA GYRASE SUBUNIT A, CHLOROPLASTIC_MITOCHONDRIAL; 1.
DR PANTHER; PTHR43493; DNA GYRASE/TOPOISOMERASE SUBUNIT A; 1.
DR Pfam; PF03989; DNA_gyraseA_C; 2.
DR Pfam; PF00521; DNA_topoisoIV; 3.
DR SMART; SM00305; HintC; 2.
DR SMART; SM00306; HintN; 2.
DR SMART; SM00434; TOP4c; 1.
DR SUPFAM; SSF101904; GyrA/ParC C-terminal domain-like; 1.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 2.
DR SUPFAM; SSF55608; Homing endonucleases; 1.
DR SUPFAM; SSF56719; Type II DNA topoisomerase; 3.
DR PROSITE; PS50818; INTEIN_C_TER; 2.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
PE 3: Inferred from homology;
KW Autocatalytic cleavage {ECO:0000256|ARBA:ARBA00022813};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Protein splicing {ECO:0000256|ARBA:ARBA00023000};
KW Reference proteome {ECO:0000313|Proteomes:UP000239494};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 371..394
FT /note="Intein C-terminal splicing"
FT /evidence="ECO:0000259|PROSITE:PS50818"
FT DOMAIN 577..633
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000259|PROSITE:PS50819"
FT DOMAIN 741..764
FT /note="Intein C-terminal splicing"
FT /evidence="ECO:0000259|PROSITE:PS50818"
FT REGION 1416..1455
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1455 AA; 155143 MW; AB786370EB82782B CRC64;
MARRKGPTTK VDPSAFDQAG ANVVDNPLKS EIEDSYLEYA YSVIHSRALP DARDGLKPVH
RRILYSMNEQ GYRPSHAYVK SARVVGDCFV RGALVSTPEG LRPIEAIGVG DLVLDAEGVA
VPVVEAYENP VSELVRVTWS TGRSMVVTPG QRFRTVNDDL TPGWTAARDL AGRLTAGYGS
ARWAAAPAQG RDARPYVQGL VSAAGRAVDG GVVVELADSA PLDVAHGWAI AAGVAASRDK
HEDPVRHTLT LTGFAVDEVP ASVPADRSTW IPFLAGLLDG AGSASPHGVL LPAAAGSAVR
AMLADLGVRA STHPEGLAVT GADAAALAAM LVRWSTTDGL HAVPATKSRA AALPGLSLTG
VTSVEPVGRD TTYDIQVGTA EHAFVVEGQV VHNCMGKYHP HGDGAIYDAM VRLAQDFSLN
TPLVDGHGNF GSPDDGPAAS RYCVVGDTRV RLADGSSTRI ADLVDLPADA EADADFEVLD
KDGKAVRVSK VFNSGVHPTL SVTTRAGFSI RGSENHLVLC LEAPMGVPLF QWRRLDEVRP
GAVVCIARNA WADVIPTARE TMLGVRGAAA VARGEAVPEF VWAGGWGVKR AFLTAVFDED
GEVRATEVGP VVHCPAGGEL AAEIQEMLAE FGVIARRTAP GLAVSGVRDL RAFVERVGFQ
NAKARRLEDL LRRPHRSAPD EIPFVAEFVG EALDLDDWET GRARIVDGIA DQETLATILP
IMDSGYRFET VTDVVPGERA EVYSVRVDTE DHSFLAGAFV NHNTEARMSE AAMLLVGELG
EDTIDFRPNY DGSLVEPSVL PAAFPNLLVN GTSGIAVGMA TNMIPHNLGE VVTAARWLIT
HPDASLDKLM EFVPGPDLPT GGMLLGLDEV RKAYETGRGV VRMRAKVDIG PLEGSRGRQA
ITVTELPYGV GSERIIEKIT DEVNKSKRLS GIADVKDLTD RENGIRLVVE CKVGVNPQAL
LADLYRLTPM EQSFGINNLV LVEGQPQTLG LKALLEVFLA HRYDVVTRRT RFRRRKREDR
LHLVEGLLTA LLDIDKVIKL IRDSENAQAA KDGLMKRFKL SEIQATYILD TPLRRLTKFD
KIELEAEQDR LRTEIAELTR ILDDPAVLRK LVSTELAAVA KDLSADRRTS LIDGDLKEVL
AASKPAGPLE VADDPCQVIL SATGLVARTA AESEEASEAR RRKGRTRHDA VAAVVHSTAR
GQILLVTNLG RAFKTDVLPL PVLPEQVGTV SLSGGMAASE LLPLEKGEKV VGIAPLGEPD
PTSPGLALGT RQGVVKVCAP EWPVRSDEFE VISLKEGDEV VGATWLTDGT ETLAFVTSDS
SLLRYAVSLV RPQGLKGGGM AGINVSAGAK VVFFGAVRPD DEVHGEAMVV TSSGQSVKVT
PFGEYPAKGR ATGGVRAHRF LKGETGLVLA WIGSRPAGSS KSGVPVELPE VDQRRDGSGS
PHPGPDVVGH VIERG
//
