ID A0A2T0TLQ6_9PSEU Unreviewed; 1195 AA.
AC A0A2T0TLQ6;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=CLV43_101931 {ECO:0000313|EMBL:PRY46652.1};
OS Umezawaea tangerina.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Umezawaea.
OX NCBI_TaxID=84725 {ECO:0000313|EMBL:PRY46652.1, ECO:0000313|Proteomes:UP000239494};
RN [1] {ECO:0000313|EMBL:PRY46652.1, ECO:0000313|Proteomes:UP000239494}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44720 {ECO:0000313|EMBL:PRY46652.1,
RC ECO:0000313|Proteomes:UP000239494};
RA Goeker M.;
RT "Genomic Encyclopedia of Archaeal and Bacterial Type Strains, Phase II
RT (KMG-II): from individual species to whole genera.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PRY46652.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; PVTF01000001; PRY46652.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2T0TLQ6; -.
DR Proteomes; UP000239494; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000239494}.
FT DOMAIN 647..808
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 826..983
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1195 AA; 129747 MW; B5C2AB57E6841459 CRC64;
MPQLGPLSGL LTTVLSDKAL RALVDAAGDP SLELEGPPAA RPLVTAALAS DQGADRTVLV
VTATGREADD LTAELRDLIG PDAVVHFPSW ETLPHERLSP RADTVGARLQ ILRRLAHPEE
HPAGALKVVV TTVRSLIQPM APGLGELKPV HLEVGVELDF AELVGRLVDV AYARVDMVEK
RGEFAVRGGI LDVFPPTAEH PLRVEFWGDE VSEIRPFSVA DQRSLPTEVR EFTAPPCREM
LLTDGVKARA AALAEEHQGD AHLVEMLDKI VDGIPSEGME ALIPALCEGE LQMLTDIVPD
GTQVLLADPE KIRARAQDLV RTGQEFLEAS WMAAAGGGKA PIDLGASAYR SLAEAADAAR
AKGRPWWTLS QLTTEGEDVL RLDLKHVEAY QGDVERAFGD LRAHVATGGT AVLVVPGSGT
AQRAAEQLRE ADVASALAED GLVGPPKAGV VTVVRGTLES GFSLPELALV VLTETDLTGG
RGGTSTKDMR RMPSKRRNSV DPLALRAGDY VVHEQHGIGK YVEMVQRTVA GATREYLVLE
YGSSKRGQPG DRLFVPTDQL DDISRYVGGE LPTLNKLGGS DWKNTKTKAR KAVKQIAAEL
VQLYAARQSA PGHAFAPDTP WQRELEDAFP FTETVDQMAA IDEVKADMER SVPMDRVICG
DVGYGKTEIA VRAAFKAVQD GKQVVVLVPT TLLAQQHLNT FAERMRAFPV TVKGLSRFTD
ALEAEQTVAG LEDGSVDVVI GTHRLLQKGL RYKDLGLVIV DEEQRFGVEH KEHIKALRTH
VDVLTMSATP IPRTLEMSLA GIREMSTILT PPEERHPILT YVGGYDDKQV GAAIRRELLR
DGQVFYVHNR VSSIERAARK IRELVPEARV VTAHGQMNED KLEKIIEGFW ENEYDVLVCT
TIVETGLDIS NANTLIVERG DMLGLSQLHQ LRGRVGRGRD RGYAYFLYPP ESPLTETAHD
RLATIAQNTE LGAGMAVAMK DLEIRGAGNI LGAEQSGHIA GVGFDLYIRL VGEAVEAFRK
HAGAESELDE ELADVRIDLP VDAHIPHDYV PGERLRLEAY RKIAAAGDAD ALTAVLDELK
DRYGAPPLPV ERLLAVAVLR QLCREHGVTE VSAQGSTIRF APLELMDSQL VRLRRLHPKA
VYKEVARTVS VPRPTEGAAG GRMGAPQLRD QELLDWCGHF LRSLSGSAVR AATGA
//
