ID A0A2T0Y540_9MICO Unreviewed; 447 AA.
AC A0A2T0Y540;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE RecName: Full=Glutaminase {ECO:0000256|ARBA:ARBA00012918, ECO:0000256|HAMAP-Rule:MF_00313};
DE EC=3.5.1.2 {ECO:0000256|ARBA:ARBA00012918, ECO:0000256|HAMAP-Rule:MF_00313};
GN Name=glsA {ECO:0000256|HAMAP-Rule:MF_00313};
GN ORFNames=BCE75_103241 {ECO:0000313|EMBL:PRZ09111.1};
OS Isoptericola sp. CG 20/1183.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
OC Promicromonosporaceae; Isoptericola.
OX NCBI_TaxID=1881052 {ECO:0000313|EMBL:PRZ09111.1, ECO:0000313|Proteomes:UP000239858};
RN [1] {ECO:0000313|EMBL:PRZ09111.1, ECO:0000313|Proteomes:UP000239858}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CG 20 {ECO:0000313|EMBL:PRZ09111.1,
RC ECO:0000313|Proteomes:UP000239858};
RA Rubin E.;
RT "Comparative analysis of microorganisms from saline springs in Andes
RT Mountain Range, Colombia.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + L-glutamine = L-glutamate + NH4(+);
CC Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=3.5.1.2;
CC Evidence={ECO:0000256|ARBA:ARBA00001062, ECO:0000256|HAMAP-
CC Rule:MF_00313};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881,
CC ECO:0000256|HAMAP-Rule:MF_00313}.
CC -!- SIMILARITY: Belongs to the glutaminase family.
CC {ECO:0000256|ARBA:ARBA00011076, ECO:0000256|HAMAP-Rule:MF_00313}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PRZ09111.1}.
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DR EMBL; PVTW01000003; PRZ09111.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2T0Y540; -.
DR OrthoDB; 9788822at2; -.
DR Proteomes; UP000239858; Unassembled WGS sequence.
DR GO; GO:0004359; F:glutaminase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:InterPro.
DR CDD; cd07042; STAS_SulP_like_sulfate_transporter; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR Gene3D; 3.30.750.24; STAS domain; 1.
DR HAMAP; MF_00313; Glutaminase; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR015868; Glutaminase.
DR InterPro; IPR002645; STAS_dom.
DR InterPro; IPR036513; STAS_dom_sf.
DR NCBIfam; TIGR03814; Gln_ase; 1.
DR PANTHER; PTHR12544; GLUTAMINASE; 1.
DR PANTHER; PTHR12544:SF29; GLUTAMINASE; 1.
DR Pfam; PF04960; Glutaminase; 1.
DR Pfam; PF01740; STAS; 1.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR SUPFAM; SSF52091; SpoIIaa-like; 1.
DR PROSITE; PS50801; STAS; 1.
PE 3: Inferred from homology;
KW Acetylation {ECO:0000256|HAMAP-Rule:MF_00313};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00313};
KW Reference proteome {ECO:0000313|Proteomes:UP000239858}.
FT DOMAIN 330..396
FT /note="STAS"
FT /evidence="ECO:0000259|PROSITE:PS50801"
FT BINDING 64
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00313"
FT BINDING 114
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00313"
FT BINDING 160
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00313"
FT BINDING 167
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00313"
FT BINDING 191
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00313"
FT BINDING 243
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00313"
FT BINDING 261
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00313"
SQ SEQUENCE 447 AA; 47408 MW; ED17DD9144459BE6 CRC64;
MRSMIPDYLD GALSDVAADD SGETARYIPE LAAADPDRLG AVFATVDGQV YGAGDVEVEF
TIQSISKPFA YALALLDRGF EPVLAKVGVE PSGEAFNEIS LEDRTGRPLN PMINAGAMTA
HALTGPEGSG PAARVERVID GLSAFAGRRL RVDEATCASE MEHAHRNLAI AHMLRSHEIL
TEDPRGVVEG YLRQCSVLVT ARDLALMAAT LANRGVNPLS GERVVGERVV RQVLSVMATC
GMYDAAGDWA TQVGIPAKSG VAGGLIGALP GQLGIATFSP RLDAHGNSVR GVSLFERFSS
DMGLHVMEVP PAARAVVRSN RVHGDAEGAV RVLRLQGGIR FAGAERVVRE VTDRPPAEPR
VVIDVSAVYS IDDVSRRMLL EVVRRLTLEG REVYLVDPEG IVPDPDPGEG GRLTVVDAVG
EIQTHTDHAE LVAVVGGGEV HAAERAW
//
