ID A0A2T4IDH7_9RHOO Unreviewed; 1048 AA.
AC A0A2T4IDH7;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=Type I restriction enzyme endonuclease subunit {ECO:0000256|RuleBase:RU364115};
DE Short=R protein {ECO:0000256|RuleBase:RU364115};
DE EC=3.1.21.3 {ECO:0000256|RuleBase:RU364115};
GN ORFNames=C8261_12560 {ECO:0000313|EMBL:PTD95827.1};
OS Pseudothauera lacus.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales; Zoogloeaceae;
OC Pseudothauera.
OX NCBI_TaxID=2136175 {ECO:0000313|EMBL:PTD95827.1, ECO:0000313|Proteomes:UP000241193};
RN [1] {ECO:0000313|EMBL:PTD95827.1, ECO:0000313|Proteomes:UP000241193}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=D20 {ECO:0000313|EMBL:PTD95827.1,
RC ECO:0000313|Proteomes:UP000241193};
RA Keele B.F.;
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:PTD95827.1, ECO:0000313|Proteomes:UP000241193}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=D20 {ECO:0000313|EMBL:PTD95827.1,
RC ECO:0000313|Proteomes:UP000241193};
RA Liang Q.-Y.;
RT "Thauera lacus sp. nov., isolated from an saline lake in Inner Mongolia,
RT China.";
RL Submitted (APR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Subunit R is required for both nuclease and ATPase
CC activities, but not for modification. {ECO:0000256|RuleBase:RU364115}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give random double-stranded
CC fragments with terminal 5'-phosphates, ATP is simultaneously
CC hydrolyzed.; EC=3.1.21.3; Evidence={ECO:0000256|ARBA:ARBA00000851,
CC ECO:0000256|RuleBase:RU364115};
CC -!- SUBUNIT: The type I restriction/modification system is composed of
CC three polypeptides R, M and S. {ECO:0000256|RuleBase:RU364115}.
CC -!- SIMILARITY: Belongs to the HsdR family. {ECO:0000256|ARBA:ARBA00008598,
CC ECO:0000256|RuleBase:RU364115}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PTD95827.1}.
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DR EMBL; PZKC01000010; PTD95827.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2T4IDH7; -.
DR OrthoDB; 9758243at2; -.
DR Proteomes; UP000241193; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0009035; F:type I site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR CDD; cd18030; DEXHc_RE_I_HsdR; 1.
DR CDD; cd22332; HsdR_N; 1.
DR CDD; cd18800; SF2_C_EcoR124I-like; 1.
DR Gene3D; 3.90.1570.50; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR007409; Restrct_endonuc_type1_HsdR_N.
DR InterPro; IPR004473; Restrct_endonuc_typeI_HsdR.
DR InterPro; IPR040980; SWI2_SNF2.
DR InterPro; IPR021810; T1RH-like_C.
DR NCBIfam; TIGR00348; hsdR; 1.
DR PANTHER; PTHR30195:SF15; TYPE I RESTRICTION ENYME HINDI ENDONUCLEASE SUBUNIT; 1.
DR PANTHER; PTHR30195; TYPE I SITE-SPECIFIC DEOXYRIBONUCLEASE PROTEIN SUBUNIT M AND R; 1.
DR Pfam; PF04313; HSDR_N; 1.
DR Pfam; PF18766; SWI2_SNF2; 1.
DR Pfam; PF11867; T1RH-like_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU364115};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU364115};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Helicase {ECO:0000313|EMBL:PTD95827.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU364115};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU364115};
KW Reference proteome {ECO:0000313|Proteomes:UP000241193};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747,
KW ECO:0000256|RuleBase:RU364115}.
FT DOMAIN 282..486
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
SQ SEQUENCE 1048 AA; 117305 MW; 43294040C14AE3B2 CRC64;
MTEDQLEQEA LGWLADVGYT HRYGLDIAPD GDAPERAAYT QTLLPFRLRE AIHRLNPQIP
TSAREDALKQ VMELGVPALL AANRQFHKLL VNGVPVQYEV DGETRGDFVR LIDWERPDRN
EWLAINQFSI KGPHHTRRPD IILFINGLPL VLIELKNPAD ANADIWKAFD QIQTYKEQIA
DVFQYNELLV ISDGSEARLG SLSASAERFM QWRTIDGVTL DPLGQFNELE TLVRGVLAPA
CLLDYLRYFV LFEDDGTLVK KVAGYHQFHA VRAAIQQVVD ASRPGGSHKG GVVWHTQGSG
KSITMTCFAA RVMREAAMEN PTIVVITDRN DLDGQLFGVF SLAQDLLREQ PVQATTRQEL
RALLGNRPSG GIVFATIQKF MPGEDEDVFP LLSDRHNIVV IADEAHRTQY GFEAKLKIVK
PRAIAAAAAN DTQALLAAEP VARYQVGYAQ HLRDALPHAT FVAFTGTPVS SEDRDTRAVF
GDYIHVYDMQ QAKEDGATVA IYFESRLAKL SLKQEDLPAI DDEVDELAED EEESQQARLK
SKWAALEKVV GAEPRIASVA ADLVAHFEER NQAQSGKAMV VAMSRDICVH LYNEIIKLRP
EWHSPDPEQG AIKIVMTGSA SDKALLRPHI YSAQVKKRLE KRFKDPADPL RLVIVRDMWL
TGFDAPCVHT LYVDKPMKGH NLMQAIARVN RVFKDKQGGL VVDYIGIANE LKSALKEYTA
SKGRGRPTVD AAEAYAVLEE KLDILRALLH GYDYSDFLTA SHKRLAGAAN HVLGQKDGKK
RFADTALAMS KAFTLCCTLD EAKALREEVA FMQAVKVILT KRDLSQQKKT DEQRELAIRQ
IIGAAVVSED VVDVFDAVGL DKPNIGILDD AFLAEVRNLP ERNLAVELLE RLLEGEIKSR
FGGNLVQEKK FSELLANVIK RYQNRAIETA QVIEELIGMA KKFRAAASRG EQLGLTEDEI
RFYDALADNE SAVRELSDET LKKIAHELTE NLRQNLSVDW SARESVRAKL RIMVKRILRK
YKYPPDQQEA AVELVLQQAK ALGEMWMT
//
