ID A0A2T5BE54_MYCDI Unreviewed; 992 AA.
AC A0A2T5BE54;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=C7449_102107 {ECO:0000313|EMBL:PTM97238.1};
OS Mycoplana dimorpha.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Mycoplana.
OX NCBI_TaxID=28320 {ECO:0000313|EMBL:PTM97238.1, ECO:0000313|Proteomes:UP000241247};
RN [1] {ECO:0000313|EMBL:PTM97238.1, ECO:0000313|Proteomes:UP000241247}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 7138 {ECO:0000313|EMBL:PTM97238.1,
RC ECO:0000313|Proteomes:UP000241247};
RA Goeker M.;
RT "Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing the
RT most valuable type-strain genomes for metagenomic binning, comparative
RT biology and taxonomic classification.";
RL Submitted (APR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SUBUNIT: Single-chain monomer with multiple functions.
CC {ECO:0000256|ARBA:ARBA00011541}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PTM97238.1}.
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DR EMBL; PZZZ01000002; PTM97238.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2T5BE54; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000241247; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06139; DNA_polA_I_Ecoli_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nuclease {ECO:0000256|ARBA:ARBA00022839};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000241247};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 5..268
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 374..577
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 746..952
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 992 AA; 108215 MW; B09041262930D53B CRC64;
MKNGDHLFLV DGSGFIFRAF HAIPPLNRKS DGLPVNAVSG FCNMLWKLLK DARSTDVGVT
PTHFAVIFDY SSKTFRNELY DQYKANRTAP PDDLIPQFGL IRHATRAFNL PCIEKEGYEA
DDLIATYARL AEEAGADVTI ISSDKDLMQL VTSKVSMYDS MKDKQIAIPE VVEKWGVTPD
KMIDLQAMTG DSTDNIPGIP GIGPKTAAQL LQEFGDLDTL LARADEIKQQ KRRENIIANR
EQALLSRQLV TLKTDTPLDT PLDELQLEPQ DGPRLIAFLK AMEFNALTRR VAEACNCDAS
AIEPAFVPVE WGTDAHGPDL DIVAGRPLTD DALSAAQAAG TLLPDASPDA PTPAALAKAR
AAFFSGRPFD RSAYVTIRDL ATLDLWLAAA RETGLVGFAA ATTTADAMQA ELCGFSLAIA
DNAKDPAGAS IRAAYVPMQH RTGDGDLLGG GLAEGQIPIG ESLARLRQLV EDPSILKVAQ
NLKFDYLVLK RHGMAIQSFD DAMLISYALD AGAAAHGIDS LSERWLAHKP IALKDVAGSG
KGAQSFDLVD IDRATAFVAE DADITLRLWH VLKPRLAAEG LTTVYERLER PLVPVLARME
ERGITIDRQV LSRLSGELGQ GAAALEDEIY QLAGERFTIG SPKQLGDILF GKMGLPGGAK
TKTGQWSTSA QVLEDLAAEG VPLPRKIVDW RQLTKLKSTY TDALPGYVHP QTRRVHTCYS
LASTTTGRLS STEPNLQNIP IRTGEGRKIR AAFIASPGHK LVSADYSQIE LRVLAHVADI
PQLRQAFAEG IDIHAMTASE MFGVPVEGMP AEVRRRAKAI NFGIIYGISA FGLANQLSIE
RSEAGDYIKR YFERFPGIRD YMESTKAFAR ENGYVETIFG RRAHYPEIRS SNPSVRAFNE
RAAINAPIQG SAADIIRRAM IKMEPALEAG KLSARMLLQV HDELVFEVEE TEVERTLPVI
VEVMENAAMP ALAMKVPLKV DARAADNWDE AH
//
