ID A0A2T5HN19_9PSED Unreviewed; 986 AA.
AC A0A2T5HN19;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=C8K61_102158 {ECO:0000313|EMBL:PTQ72949.1};
OS Pseudomonas sp. GV071.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=2135754 {ECO:0000313|EMBL:PTQ72949.1, ECO:0000313|Proteomes:UP000244119};
RN [1] {ECO:0000313|EMBL:PTQ72949.1, ECO:0000313|Proteomes:UP000244119}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GV071 {ECO:0000313|EMBL:PTQ72949.1,
RC ECO:0000313|Proteomes:UP000244119};
RA Pelletier D.;
RT "Populus root and rhizosphere microbial communities from Tennessee, USA.";
RL Submitted (APR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SUBUNIT: Single-chain monomer with multiple functions.
CC {ECO:0000256|ARBA:ARBA00011541}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PTQ72949.1}.
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DR EMBL; QAOJ01000002; PTQ72949.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2T5HN19; -.
DR Proteomes; UP000244119; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06139; DNA_polA_I_Ecoli_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nuclease {ECO:0000256|ARBA:ARBA00022839};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000244119};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 52..314
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 379..574
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 744..950
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 986 AA; 107906 MW; D7822391264E694B CRC64;
MKLLTRHSGA FDLLASDYKS QSLRRPPVAR YIFPLSVHHG PSNRLILRMS QTPLVLVDGS
SYLYRAFHAL PPLTTSKGLP TGAVKGVLNM LKSLRKQYPQ SPFAVVFDAK GGTFRDELFA
DYKANRPSMP DELRVQIEPL HASVRALGYP LLCVDGVEAD DVIGTLARQC AAAGRDVVIS
TGDKDMAQLV CPHVTLVNTM SGSVYDIDGV KEKFGVGPEL IIDYLALMGD KVDNIPGVPG
VGEKTAVGLL VGVGGGLEVL YANLDKVPEL PIRGAKNLPA KLLEHKDMAF LSYQLATIKL
DVPLNIEIDS LHPGEQDREA LAELYALLEF KTGLDELARD VKAAGIAAIR SGGLFDAPAA
EPPATEENVA AVESAANQYE TVLDAERFAV WLEKLNKAEL FAFDTETTGL DAQQAQLVGI
SIAVQVNEAA YIPLTHSYMG VPAQLDRDTV LKALKPILED PNKAKVGQHA KYDINILANC
AIDGESNNGI TLRGVRFDSM LESYVLDSVA TRHNMDALAL KYLDHTTIAF EDIAGKGAKQ
LTFDQIALEQ AGPYAAEDAD ITLRLHQVLW QKLQAVPSLA KVLSDIEMPL VPVLARIERN
GALVDANLLG LQSNELGEKM VQLEREAFAI AGEEFNLSSP KQLGVILYEK LGMPVLSKTA
KGQASTAEAV LAELAEQDFP LPKVLMQYRS MSKLKSTYTD RLPEQINPRT GRIHTSYHQA
VAATGRLSST DPNLQNIPIR TAEGRRIRQA FIAPPGYKLL AADYSQIELR IMAHLAKDES
LLHAFRDNLD VHRATAAEVF GVELEQVTTD MRRKAKAINF GLIYGMSAFG LAKQIGVDRK
ESQAYIDRYF ARYPGVLSYM ERTREQASEQ GYVETLFGRR LYLPEIHSKN AGLRKGAERT
AINAPMQGTA ADIIKRAMVK VDDWLSESGL DAKVILQVHD ELVLEVREDL LETVSAEIRQ
RMSAAAELDV PLLVEVGVGA NWDEAH
//
