ID A0A2T5J5C2_9SPHI Unreviewed; 505 AA.
AC A0A2T5J5C2;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=Probable membrane transporter protein {ECO:0000256|RuleBase:RU363041};
GN ORFNames=C8P68_11051 {ECO:0000313|EMBL:PTQ92920.1};
OS Mucilaginibacter yixingensis.
OC Bacteria; Bacteroidota; Sphingobacteriia; Sphingobacteriales;
OC Sphingobacteriaceae; Mucilaginibacter.
OX NCBI_TaxID=1295612 {ECO:0000313|EMBL:PTQ92920.1, ECO:0000313|Proteomes:UP000244168};
RN [1] {ECO:0000313|EMBL:PTQ92920.1, ECO:0000313|Proteomes:UP000244168}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 26809 {ECO:0000313|EMBL:PTQ92920.1,
RC ECO:0000313|Proteomes:UP000244168};
RA Goeker M.;
RT "Genomic Encyclopedia of Archaeal and Bacterial Type Strains, Phase II
RT (KMG-II): from individual species to whole genera.";
RL Submitted (APR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin;
CC Xref=Rhea:RHEA:15613, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58351, ChEBI:CHEBI:58827; EC=1.3.1.76;
CC Evidence={ECO:0000256|ARBA:ARBA00001156};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; siroheme
CC biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
CC {ECO:0000256|ARBA:ARBA00005010}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|RuleBase:RU363041};
CC Multi-pass membrane protein {ECO:0000256|RuleBase:RU363041}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the 4-toluene sulfonate uptake permease (TSUP)
CC (TC 2.A.102) family. {ECO:0000256|RuleBase:RU363041}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PTQ92920.1}.
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DR EMBL; QAOQ01000010; PTQ92920.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2T5J5C2; -.
DR OrthoDB; 45564at2; -.
DR UniPathway; UPA00262; UER00222.
DR Proteomes; UP000244168; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004325; F:ferrochelatase activity; IEA:InterPro.
DR GO; GO:0043115; F:precorrin-2 dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0019354; P:siroheme biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR028161; Met8-like.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR028281; Sirohaem_synthase_central.
DR InterPro; IPR006367; Sirohaem_synthase_N.
DR InterPro; IPR002781; TM_pro_TauE-like.
DR NCBIfam; TIGR01470; cysG_Nterm; 1.
DR PANTHER; PTHR35330; SIROHEME BIOSYNTHESIS PROTEIN MET8; 1.
DR PANTHER; PTHR35330:SF1; SIROHEME BIOSYNTHESIS PROTEIN MET8; 1.
DR Pfam; PF13241; NAD_binding_7; 1.
DR Pfam; PF14824; Sirohm_synth_M; 1.
DR Pfam; PF01925; TauE; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF75615; Siroheme synthase middle domains-like; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|RuleBase:RU363041};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU363041};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Porphyrin biosynthesis {ECO:0000256|ARBA:ARBA00023244};
KW Reference proteome {ECO:0000313|Proteomes:UP000244168};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU363041};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU363041}.
FT TRANSMEM 221..240
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 255..279
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 324..343
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 349..369
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 389..417
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 429..447
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 454..474
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 480..502
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT DOMAIN 144..165
FT /note="Siroheme synthase central"
FT /evidence="ECO:0000259|Pfam:PF14824"
SQ SEQUENCE 505 AA; 54612 MW; 012D8A394BD63515 CRC64;
MTLLPKDNQA QSAGEAAAQG NNTLFPVFLK LHDLHTVVIG AGNVGLEKLN AVLNNSPQAK
VTVIAQAFLP QVHELIAKYP NVTAQQKTFI DTDLDHADLV IAATGSPELN DYIRQSARER
KLLVNIADKP ALCDFYLASI VQKGDLKIAI STNGKSPTVA KRLKEVLNET IPDEIDTTLQ
QVNQIRETLN GDFSEKVHQL NKLTAVLVEP KVEQPKPNFQ WIIWGAIITS VVITVGALWY
RNPEANAFIR GIDPIFYYFL GAGFVFAMID GAIGMSYGVT STSFSLSMGI PPASASMGVH
LSEIMSNGIA GWMHYRLGNI NRKLFWLLVI PGAAGAMIGA YLLSSLEHYS TYTKPFVSLY
TLILGIVILK KAFKTGAKKS ADKIKRIRLL GFGGGFIDAV GGGGWGSIVL SSLIAGGRHP
RFSLGTVKLS RFFIAMVSSM TFIFMLSRDA RWDAIAGLII GSALASPIAV RVANKISAKS
IMVAVGIIVI ATSIRSIILF LLKIL
//
