ID A0A2T6CMS6_9BACT Unreviewed; 473 AA.
AC A0A2T6CMS6;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 24-JAN-2024, entry version 22.
DE RecName: Full=Tyrosine phenol-lyase {ECO:0000256|ARBA:ARBA00016166};
DE EC=4.1.99.2 {ECO:0000256|ARBA:ARBA00013154};
DE AltName: Full=Beta-tyrosinase {ECO:0000256|ARBA:ARBA00033449};
GN ORFNames=DB354_16410 {ECO:0000313|EMBL:PTX91471.1};
OS Opitutus sp. ER46.
OC Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae; Opitutus.
OX NCBI_TaxID=2161864 {ECO:0000313|EMBL:PTX91471.1, ECO:0000313|Proteomes:UP000244023};
RN [1] {ECO:0000313|EMBL:PTX91471.1, ECO:0000313|Proteomes:UP000244023}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ER46 {ECO:0000313|EMBL:PTX91471.1,
RC ECO:0000313|Proteomes:UP000244023};
RA Buenger W., Mueller J., Hurek T., Reinhold-Hurek B.;
RT "Genomic and phenotypic characterization of four novel plant-associated
RT Verrucomicrobia indicate a new subdivision with one cultivated member.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + L-tyrosine = NH4(+) + phenol + pyruvate;
CC Xref=Rhea:RHEA:21704, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15882, ChEBI:CHEBI:28938, ChEBI:CHEBI:58315; EC=4.1.99.2;
CC Evidence={ECO:0000256|ARBA:ARBA00001821};
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000256|ARBA:ARBA00001933,
CC ECO:0000256|PIRSR:PIRSR611166-50};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC -!- SIMILARITY: Belongs to the beta-eliminating lyase family.
CC {ECO:0000256|ARBA:ARBA00009721}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PTX91471.1}.
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DR EMBL; QAYX01000024; PTX91471.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2T6CMS6; -.
DR InParanoid; A0A2T6CMS6; -.
DR OrthoDB; 9764079at2; -.
DR Proteomes; UP000244023; Unassembled WGS sequence.
DR GO; GO:0050371; F:tyrosine phenol-lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0006570; P:tyrosine metabolic process; IEA:InterPro.
DR CDD; cd00617; Tnase_like; 1.
DR Gene3D; 3.90.1150.10; Aspartate Aminotransferase, domain 1; 1.
DR Gene3D; 3.40.640.10; Type I PLP-dependent aspartate aminotransferase-like (Major domain); 1.
DR InterPro; IPR001597; ArAA_b-elim_lyase/Thr_aldolase.
DR InterPro; IPR011166; Beta-eliminating_lyase.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR InterPro; IPR018176; Tryptophanase_CS.
DR InterPro; IPR013441; Tyr_phenol_ly.
DR NCBIfam; TIGR02618; tyr_phenol_ly; 1.
DR PANTHER; PTHR32325; BETA-ELIMINATING LYASE-LIKE PROTEIN-RELATED; 1.
DR PANTHER; PTHR32325:SF4; TRYPTOPHANASE; 1.
DR Pfam; PF01212; Beta_elim_lyase; 1.
DR PIRSF; PIRSF001386; Trpase; 1.
DR SUPFAM; SSF53383; PLP-dependent transferases; 1.
DR PROSITE; PS00853; BETA_ELIM_LYASE; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000313|EMBL:PTX91471.1};
KW Pyridoxal phosphate {ECO:0000256|ARBA:ARBA00022898,
KW ECO:0000256|PIRSR:PIRSR611166-50};
KW Reference proteome {ECO:0000313|Proteomes:UP000244023}.
FT DOMAIN 65..439
FT /note="Aromatic amino acid beta-eliminating lyase/threonine
FT aldolase"
FT /evidence="ECO:0000259|Pfam:PF01212"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 274
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000256|PIRSR:PIRSR611166-50"
SQ SEQUENCE 473 AA; 52934 MW; C0284F1645DC3517 CRC64;
MPPEPAPSTP IRTTGQQLRR RSWAEPWKIK MVEPIKVTTA AQRATALRDA GYNTFLLRSE
DVYIDLLTDS GTSAMSDWQW AGLMLGDEAY AGSKNFYHLE DTVRRHYGYK HLIPTHQGRG
AEHILSQIMI QPGDTVPGNM YFTTTRYHQE HAGAQFVDVV VDEGHDPANP APFKGNVDLA
KLERVLTTAK HVPYVCVAAT VNMAGGQPIS MANMRAVREL CARQHVPIML DATRAVENAW
FIKTREPGYA ERSCGEILRE FCSYSDGCTM SGKKDPLVNI GGWLSLNDDQ LADKARNMVV
VYEGLHTYGG MAGRDMEAMA RGIEESVQDD HMRARIGQVE YLGELLQVAG VPVVLPIGGH
AVYLDARRFY PHLTQDEFPA QTLAAELYLD AGVRAMERGI VSAGRDHKTG KHHYPKLELV
RLTIPRRVYT QAHMDVVAES VTAVFQNPDK PRGLKMVYEP ENLRFFQARF EPL
//
