ID A0A2T6DQV3_9BACT Unreviewed; 934 AA.
AC A0A2T6DQV3;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 24-JAN-2024, entry version 17.
DE RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891};
DE EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891};
DE AltName: Full=Omega-protein {ECO:0000256|ARBA:ARBA00032877};
DE AltName: Full=Relaxing enzyme {ECO:0000256|ARBA:ARBA00032235};
DE AltName: Full=Swivelase {ECO:0000256|ARBA:ARBA00030003};
DE AltName: Full=Untwisting enzyme {ECO:0000256|ARBA:ARBA00031985};
GN ORFNames=DB347_19155 {ECO:0000313|EMBL:PTY04822.1};
OS Opitutaceae bacterium EW11.
OC Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae.
OX NCBI_TaxID=2161865 {ECO:0000313|EMBL:PTY04822.1, ECO:0000313|Proteomes:UP000244239};
RN [1] {ECO:0000313|EMBL:PTY04822.1, ECO:0000313|Proteomes:UP000244239}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EW11 {ECO:0000313|EMBL:PTY04822.1,
RC ECO:0000313|Proteomes:UP000244239};
RA Buenger W., Mueller J., Hurek T., Reinhold-Hurek B.;
RT "Genomic and phenotypic characterization of four novel plant-associated
RT Verrucomicrobia indicate a new subdivision with one cultivated member.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213};
CC -!- SIMILARITY: Belongs to the type IA topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00009446}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PTY04822.1}.
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DR EMBL; QAYY01000013; PTY04822.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2T6DQV3; -.
DR OrthoDB; 9803554at2; -.
DR Proteomes; UP000244239; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:UniProtKB-EC.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00186; TOP1Ac; 1.
DR CDD; cd03362; TOPRIM_TopoIA_TopoIII; 1.
DR Gene3D; 3.40.50.140; -; 1.
DR Gene3D; 1.10.460.10; Topoisomerase I, domain 2; 1.
DR Gene3D; 2.70.20.10; Topoisomerase I, domain 3; 1.
DR Gene3D; 1.10.290.10; Topoisomerase I, domain 4; 1.
DR InterPro; IPR000380; Topo_IA.
DR InterPro; IPR003601; Topo_IA_2.
DR InterPro; IPR023406; Topo_IA_AS.
DR InterPro; IPR013497; Topo_IA_cen.
DR InterPro; IPR013824; Topo_IA_cen_sub1.
DR InterPro; IPR013825; Topo_IA_cen_sub2.
DR InterPro; IPR013826; Topo_IA_cen_sub3.
DR InterPro; IPR023405; Topo_IA_core_domain.
DR InterPro; IPR003602; Topo_IA_DNA-bd_dom.
DR InterPro; IPR005738; TopoIII.
DR InterPro; IPR025589; Toprim_C_rpt.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR034144; TOPRIM_TopoIII.
DR NCBIfam; TIGR01056; topB; 1.
DR PANTHER; PTHR11390:SF21; DNA TOPOISOMERASE 3-ALPHA; 1.
DR PANTHER; PTHR11390; PROKARYOTIC DNA TOPOISOMERASE; 1.
DR Pfam; PF01131; Topoisom_bac; 1.
DR Pfam; PF01751; Toprim; 1.
DR Pfam; PF13342; Toprim_Crpt; 3.
DR PRINTS; PR00417; PRTPISMRASEI.
DR SMART; SM00437; TOP1Ac; 1.
DR SMART; SM00436; TOP1Bc; 1.
DR SMART; SM00493; TOPRIM; 1.
DR SUPFAM; SSF56712; Prokaryotic type I DNA topoisomerase; 1.
DR PROSITE; PS00396; TOPOISOMERASE_I_PROK; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:PTY04822.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000244239};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 2..136
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
FT REGION 456..481
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 618..639
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 934 AA; 104465 MW; 3AAF83E7D0887F02 CRC64;
MKSLVIAEKP SVAADLARSL GKVPKKGDHY ENDEYVITSA VGHLVELLMP EDIDKKKYGF
WRLETLPIIP EKFELKPIQD AKDRFDHLKK LLQRKDVDQV VNACDAGREG ELIFAYIYQL
AKAKLPVKRA WMQTMTTEGI REAFKHLRDG AEMAGLADAA RCRSESDWLI GINGTRAITK
RMFGSRAGNV ASVGRVQTPT LAIVFARELE IRNFKPRGYW RVTATFQVTK GNYEGVYQRP
NYRKSDSDEH DRIDRIWDQA TAEAIVAACQ GMPLASVSEE KKSATQSSPR LYDLTTLQRE
ANNRFGLSAR RTLQIAQALY EKHKMITYPR TDSRALPEDY MPTVRQALGN LQGDLAVHAQ
KVLGEGWVRP NKRIFNNAQI SDHFAIIPTD AEPKNLEEME AKIYDMIARR FVATFFPPAE
FDITTRLSTV AGHQFKTEGK VLTSPGWLAV YGKTTVDDDS PDSKALPPID PADGNPPQAK
TLGAQLHSET TKPPPRYTEA TLLSAMEGAG KLVDDENLAE AMKERGLGTP ATRADTIDGL
INQKYIERQQ RELVPTAKAE SLLQFLAAVK ADALTKPDMT GEWEHKLRQM EHGKFPRDRF
MSEIVDVTKG IVDRTKTFEE DASTARQTDI PSPTDGKPML ETLRGYKSQD GTLMIYKVMS
GRKLEENEVR ELVANGQIGP LDGFVSAKTG NRFPATVRLV DDPKEAGKRK AELDFGNKYD
VNTIEPFWTD PKTGAELCEA PTSYVLRERE GEGWKVAFQV GRLMCQKPIP REEAIRLIEA
GKTELIKGFI SKKGRPFDAY LVRQGPKVSW EFPPRKTAAK DGEVGAKTRR TKAPLDLTKA
VRIGESKAHG GELLQTPESF VVTKPSGDGS PRVVFELKRT LCQKEIPSDE VERLVETGKT
GLIEGFVSKR GSTFSAYLTL SKDKKKAEFE FPPR
//
