ID A0A2T6FEV4_9GAMM Unreviewed; 594 AA.
AC A0A2T6FEV4;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN ORFNames=B0W54_21350 {ECO:0000313|EMBL:PUA26952.1};
OS Cellvibrio sp. 79.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
OC Cellvibrionaceae; Cellvibrio.
OX NCBI_TaxID=1954207 {ECO:0000313|EMBL:PUA26952.1, ECO:0000313|Proteomes:UP000243979};
RN [1] {ECO:0000313|EMBL:PUA26952.1, ECO:0000313|Proteomes:UP000243979}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=79 {ECO:0000313|EMBL:PUA26952.1};
RA Zhang Y., Xu J., Wang N.;
RT "Cellvibrio sp. 79 recovered from metagenome.";
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|PROSITE-ProRule:PRU10060, ECO:0000256|RuleBase:RU361166}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PUA26952.1}.
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DR EMBL; MVDH01000005; PUA26952.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2T6FEV4; -.
DR Proteomes; UP000243979; Unassembled WGS sequence.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR CDD; cd02850; E_set_Cellulase_N; 1.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR004197; Cellulase_Ig-like.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR Pfam; PF02927; CelD_N; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00698; GH9_3; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10060};
KW Cellulose degradation {ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10060};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10060};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10060};
KW Reference proteome {ECO:0000313|Proteomes:UP000243979};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..22
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 23..594
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5015375917"
FT DOMAIN 46..124
FT /note="Cellulase Ig-like"
FT /evidence="ECO:0000259|Pfam:PF02927"
FT DOMAIN 137..584
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT REGION 511..546
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 563
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 572
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 594 AA; 64163 MW; 498437E881D387C7 CRC64;
MSFPPKYLAR IIAVVSLSSL LAACGNDGNS NGSSSVAGAA NSSASSSLIK VNQLGFLPES
SKLAVVPDVP ATSFKLLKAG TAIEVFSGTL SAAKVWDASR ESVKVADFSS VKTPGDYQLH
VDGLANSHVF SIAPDVYNAL NAASIKAFYF NRSGVELLPE FAGKFARPLG HADTQVLIHP
SAASDARPSG TVVSSPKGWY DAGDYNKYIV NSGISTYTLL AAYEHFPEIF NNQNLTIPES
GNALPDLLDE TLWNLEWMLT MQDPNDGGVY HKLTNENFDG TIMPHEALRE RYLVQKTTAA
TLDFAAVMAT ASRVFAPYET QLPGLSAKMR KAAESAWEWA NIHPAIVYKQ PSDVRTGEYG
DKTLSDEFAW AAAELYITTR VDGYYTALKP VDVFNSVPSW GNVNGLAWVS LAHHRAQLTN
VADQTLIASR IKTLADNLLS TWYASPYKVS MEKHDFVWGS NSGALNQAMM LVQAYRLNGK
RDYLDAAQAQ LDYVLGRNAT DTSFVTGFGE KSTLHPHHRP SEADGIPEPI PGFIAGGPQP
GQQDKADCKA VYPSSVAAKS YLDNYCSYAS NEIAINWNAP LVYVSAAIAA LTPK
//
