ID   A0A2T9XSZ9_9CELL        Unreviewed;       555 AA.
AC   A0A2T9XSZ9;
DT   18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT   18-JUL-2018, sequence version 1.
DT   27-MAR-2024, entry version 18.
DE   SubName: Full=3-oxosteroid 1-dehydrogenase {ECO:0000313|EMBL:PVU83192.1};
GN   ORFNames=DDP54_09490 {ECO:0000313|EMBL:PVU83192.1};
OS   Cellulomonas sp. WB94.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Cellulomonadaceae;
OC   Cellulomonas.
OX   NCBI_TaxID=2173174 {ECO:0000313|EMBL:PVU83192.1, ECO:0000313|Proteomes:UP000245142};
RN   [1] {ECO:0000313|EMBL:PVU83192.1, ECO:0000313|Proteomes:UP000245142}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WB94 {ECO:0000313|EMBL:PVU83192.1,
RC   ECO:0000313|Proteomes:UP000245142};
RA   Anderson E., Ishii S., Jang J.;
RT   "Cellulomonas - woodchip bioreactor.";
RL   Submitted (APR-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|ARBA:ARBA00001974};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PVU83192.1}.
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DR   EMBL; QEES01000002; PVU83192.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2T9XSZ9; -.
DR   OrthoDB; 9813348at2; -.
DR   Proteomes; UP000245142; Unassembled WGS sequence.
DR   GO; GO:0033765; F:steroid dehydrogenase activity, acting on the CH-CH group of donors; IEA:UniProt.
DR   GO; GO:0008202; P:steroid metabolic process; IEA:UniProt.
DR   Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR   Gene3D; 3.90.700.10; Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain; 1.
DR   InterPro; IPR003953; FAD-binding_2.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR027477; Succ_DH/fumarate_Rdtase_cat_sf.
DR   PANTHER; PTHR43400:SF7; FAD_BINDING_2 DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR43400; FUMARATE REDUCTASE; 1.
DR   Pfam; PF00890; FAD_binding_2; 1.
DR   SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR   SUPFAM; SSF56425; Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain; 1.
PE   4: Predicted;
KW   Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Reference proteome {ECO:0000313|Proteomes:UP000245142}.
FT   DOMAIN          9..532
FT                   /note="FAD-dependent oxidoreductase 2 FAD binding"
FT                   /evidence="ECO:0000259|Pfam:PF00890"
SQ   SEQUENCE   555 AA;  59288 MW;  4C8C228B1DB0EB82 CRC64;
     MEKFDKVVDV VVVGSGSAAM TTALAVRESG KEPLVLESTE LYGGSSAMSG GGLWIPNNPV
     MREAGVEDSY EKARTYMDTV IGDVGPASSP ERRDAFLRNG PEMVSFLRGL GMKFVYARGY
     SDYYPEKPGG TPIGRGIEGE RWNVKKLGPW AGKVRGLIPM AAHTSEVGAI NLSFRTLKGF
     LTAANVVGVQ TILPLLIGQK KVGLGNSLMG QLLYLALQRD IDVWLSSPLV ELITADGAVV
     GVVVDKDGTR MRIGARQGVM LAAGGFAHND EMRQKYHPHP ITTTWTSANP GDIGTPINAG
     VAVGATLALM DDAWWGPSVL NANGSAGFHL AERSLPHGFI VDSAGERFMN ESESYVDAGH
     DQYERNAKVS AIPAYLIIDS HHRRWYPFGM ALPGMTPKKM IQSGFFTKAD TLAELAGKIG
     VEPDGLHRTA SRFAEFARTG VDEDFHRGDS AYDRVYSDPR VKPNPNLGAV SKGPFYAVKV
     WPGDLGTKGG LLTDEHARVL REDGSVIEGL YAAGNTSASV MGRTYPGPGS TIGPAMTFGY
     IGGRHVAAKG ETVPS
//