ID   A0A2U0HCM5_9MICO        Unreviewed;       821 AA.
AC   A0A2U0HCM5;
DT   18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT   18-JUL-2018, sequence version 1.
DT   27-MAR-2024, entry version 24.
DE   RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE            EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
GN   ORFNames=DEA06_03615 {ECO:0000313|EMBL:PVW06604.1};
OS   Microbacterium sp. Gd 4-13.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC   Microbacterium.
OX   NCBI_TaxID=2173179 {ECO:0000313|EMBL:PVW06604.1, ECO:0000313|Proteomes:UP000245623};
RN   [1] {ECO:0000313|EMBL:PVW06604.1, ECO:0000313|Proteomes:UP000245623}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Gd 4-13 {ECO:0000313|EMBL:PVW06604.1,
RC   ECO:0000313|Proteomes:UP000245623};
RA   Spirina E.V., Kuleshov K.V., Rivkina E.M.;
RT   "Genome sequencing and assembly of Microbacterium sp. Gd 4-13.";
RL   Submitted (APR-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP-dependent breakage, passage and rejoining of double-
CC         stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC   -!- SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit
CC       family. {ECO:0000256|ARBA:ARBA00008263}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PVW06604.1}.
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DR   EMBL; QEIJ01000001; PVW06604.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2U0HCM5; -.
DR   OrthoDB; 9806486at2; -.
DR   Proteomes; UP000245623; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0034335; F:DNA negative supercoiling activity; IEA:UniProt.
DR   GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR   CDD; cd00187; TOP4c; 1.
DR   Gene3D; 3.30.1360.40; -; 1.
DR   Gene3D; 2.120.10.90; DNA gyrase/topoisomerase IV, subunit A, C-terminal; 1.
DR   Gene3D; 3.90.199.10; Topoisomerase II, domain 5; 1.
DR   Gene3D; 1.10.268.10; Topoisomerase, domain 3; 1.
DR   InterPro; IPR006691; GyrA/parC_rep.
DR   InterPro; IPR035516; Gyrase/topoIV_suA_C.
DR   InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR   InterPro; IPR013758; Topo_IIA_A/C_ab.
DR   InterPro; IPR013757; Topo_IIA_A_a_sf.
DR   InterPro; IPR002205; Topo_IIA_dom_A.
DR   PANTHER; PTHR43493:SF5; DNA GYRASE SUBUNIT A, CHLOROPLASTIC_MITOCHONDRIAL; 1.
DR   PANTHER; PTHR43493; DNA GYRASE/TOPOISOMERASE SUBUNIT A; 1.
DR   Pfam; PF03989; DNA_gyraseA_C; 3.
DR   Pfam; PF00521; DNA_topoisoIV; 1.
DR   SMART; SM00434; TOP4c; 1.
DR   SUPFAM; SSF101904; GyrA/ParC C-terminal domain-like; 1.
DR   SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
PE   3: Inferred from homology;
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:PVW06604.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000245623};
KW   Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT   DOMAIN          21..474
FT                   /note="DNA topoisomerase type IIA"
FT                   /evidence="ECO:0000259|SMART:SM00434"
FT   REGION          780..808
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   821 AA;  87070 MW;  9D706EC418546169 CRC64;
     MPASRPSPAA AANVVERIED VDVSTEMQGS FLEYAYSVIY SRALPDARDG LKPVQRRILY
     QMAEMGLRPD RGHVKSARVV GEVMGKLHPH GDSPIYDALV RLAQSFTLRV PLVDGHGNFG
     SLDDGPAASR YTEARLAPPA LALTENLDED VVDFIPNYDG QFQQPEVLPA AFPNLLVNGA
     TGIAVGMATN MAPHNLIEVV AAAVHLLANP EASVEELMEF VPGPDLPTGG IIVGLDGIKD
     AYTNGRGTFR TRAKVSIESL GPRRTGLVVT ELPYLVGPER LVEKIKDAVT AKKLTGISDV
     TDLTDRNNGL RLIIGIKTGF DPQAVLDQLY RLTPLEDSFG INNVALVDGQ PKTLGLRDLL
     RVYIDHRIRV VTRRSEFRLA RKQERLHLVE GLLIAILDID EVIQVIRGSD DGEAARLRLM
     EVFDLSQAQA EYILELRLRR LTKFSRIELE AERDALKAEI AQLIELLSST ALLHAQVARE
     LEAAAEAYGT PRRTMLLNGG PVVPRSAKAA AAADLQIADA PCRVFLSATG RMVRAELVAD
     APDGGIVAPS RRSKHDAIRS AVDSTTRGDL GAVTTKGRLV RFTPVDLPSV PANSVQLAAG
     TRVDQYLGLP GGEHVVAIVP LDEAPPLAIG TAHGVVKRVA ASEITNKPDV EIIALKPGDR
     VVGAAPAPDD AELVFVAADA QLLRFDASAV RPQGRSAGGM AGIRLSSGVE VVAFGVVPAS
     AAQTVVVTVA GDSTALPGTD AGSAKVSAFT EYPPKGRATG GVRAQRFLRG EDALVQAWVG
     EEPRAVGPDG STRALPDAGA KRDASGSALD GSVGAIGTVV R
//