ID A0A2U1K9U5_ARTAN Unreviewed; 447 AA.
AC A0A2U1K9U5;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 08-NOV-2023, entry version 15.
DE RecName: Full=3-ketoacyl-CoA synthase {ECO:0000256|PIRNR:PIRNR036417};
DE EC=2.3.1.- {ECO:0000256|PIRNR:PIRNR036417};
GN ORFNames=CTI12_AA627830 {ECO:0000313|EMBL:PWA27278.1};
OS Artemisia annua (Sweet wormwood).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Asteroideae; Anthemideae;
OC Artemisiinae; Artemisia.
OX NCBI_TaxID=35608 {ECO:0000313|EMBL:PWA27278.1, ECO:0000313|Proteomes:UP000245207};
RN [1] {ECO:0000313|EMBL:PWA27278.1, ECO:0000313|Proteomes:UP000245207}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Huhao1 {ECO:0000313|Proteomes:UP000245207};
RC TISSUE=Leaf {ECO:0000313|EMBL:PWA27278.1};
RX PubMed=29703587; DOI=10.1016/j.molp.2018.03.015;
RA Shen Q., Zhang L., Liao Z., Wang S., Yan T., Shi P., Liu M., Fu X., Pan Q.,
RA Wang Y., Lv Z., Lu X., Zhang F., Jiang W., Ma Y., Chen M., Hao X., Li L.,
RA Tang Y., Lv G., Zhou Y., Sun X., Brodelius P.E., Rose J.K.C., Tang K.;
RT "The genome of Artemisia annua provides insight into the evolution of
RT Asteraceae family and artemisinin biosynthesis.";
RL Mol. Plant 11:776-788(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a very-long-chain acyl-CoA + H(+) + malonyl-CoA = a very-long-
CC chain 3-oxoacyl-CoA + CO2 + CoA; Xref=Rhea:RHEA:32727,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57384, ChEBI:CHEBI:90725, ChEBI:CHEBI:90736;
CC EC=2.3.1.199; Evidence={ECO:0000256|ARBA:ARBA00001906};
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005194, ECO:0000256|PIRNR:PIRNR036417}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000256|ARBA:ARBA00005531,
CC ECO:0000256|PIRNR:PIRNR036417}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PWA27278.1}.
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DR EMBL; PKPP01027920; PWA27278.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2U1K9U5; -.
DR STRING; 35608.A0A2U1K9U5; -.
DR UniPathway; UPA00094; -.
DR Proteomes; UP000245207; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0009922; F:fatty acid elongase activity; IEA:UniProtKB-EC.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR012392; 3-ktacl-CoA_syn.
DR InterPro; IPR013747; ACP_syn_III_C.
DR InterPro; IPR013601; FAE1_typ3_polyketide_synth.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR31561; 3-KETOACYL-COA SYNTHASE; 1.
DR PANTHER; PTHR31561:SF158; 3-KETOACYL-COA SYNTHASE; 1.
DR Pfam; PF08541; ACP_syn_III_C; 1.
DR Pfam; PF08392; FAE1_CUT1_RppA; 1.
DR PIRSF; PIRSF036417; 3-ktacl-CoA_syn; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|PIRNR:PIRNR036417};
KW Reference proteome {ECO:0000313|Proteomes:UP000245207};
KW Transferase {ECO:0000256|PIRNR:PIRNR036417}.
FT DOMAIN 23..308
FT /note="FAE"
FT /evidence="ECO:0000259|Pfam:PF08392"
FT DOMAIN 330..413
FT /note="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08541"
FT ACT_SITE 166
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 245
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 334
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 338
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 367
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 371
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
SQ SEQUENCE 447 AA; 50857 MW; 250E9F537CD3F691 CRC64;
MFLTFACSLA LCLLYIFYRF IDKRRSQSCY ILHYECFKPS EDRKLSIEFS VQMILQCQNL
GLEEHRFLLR AIVSSGIGED TYGPKCFFTK SGTNARLSDC QFEMDEFYIE TLDRLFSKTR
VSPQDIDILV VNVSVMTLVP SLTSRIINHY KMRDDIKSFN ISGMGCSASL ISINLVQNLF
KSNRNKLALV LTSEAISPNW YNGNEKSMIL TNCLFRCGGS SILLTNKPAL RKQAMFKLKC
LVRTHLGSSD EAHTCCQQQE DSKGNIGFFL GKTLPKTATR ALTENLRSIA PKILPLSAIF
RYVLQVNIQK LRAKYLHNTA KKRVNMNFKS GVDHFCLHPG GKAVIEGVKK SLVLTDDDVE
PSRMTLHRFG NTSASSLWYV LAYMEAKNRL RKGDRVLMIG FGSGFKCNSC TWEVLRDLDD
RNVWDDCVDR FPPNLPIYPS SRNMNGF
//
