ID   A0A2U1LM23_ARTAN        Unreviewed;       556 AA.
AC   A0A2U1LM23;
DT   18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT   18-JUL-2018, sequence version 1.
DT   08-NOV-2023, entry version 11.
DE   SubName: Full=Carotenoid oxygenase {ECO:0000313|EMBL:PWA50047.1};
GN   ORFNames=CTI12_AA476600 {ECO:0000313|EMBL:PWA50047.1};
OS   Artemisia annua (Sweet wormwood).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Asteroideae; Anthemideae;
OC   Artemisiinae; Artemisia.
OX   NCBI_TaxID=35608 {ECO:0000313|EMBL:PWA50047.1, ECO:0000313|Proteomes:UP000245207};
RN   [1] {ECO:0000313|EMBL:PWA50047.1, ECO:0000313|Proteomes:UP000245207}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Huhao1 {ECO:0000313|Proteomes:UP000245207};
RC   TISSUE=Leaf {ECO:0000313|EMBL:PWA50047.1};
RX   PubMed=29703587; DOI=10.1016/j.molp.2018.03.015;
RA   Shen Q., Zhang L., Liao Z., Wang S., Yan T., Shi P., Liu M., Fu X., Pan Q.,
RA   Wang Y., Lv Z., Lu X., Zhang F., Jiang W., Ma Y., Chen M., Hao X., Li L.,
RA   Tang Y., Lv G., Zhou Y., Sun X., Brodelius P.E., Rose J.K.C., Tang K.;
RT   "The genome of Artemisia annua provides insight into the evolution of
RT   Asteraceae family and artemisinin biosynthesis.";
RL   Mol. Plant 11:776-788(2018).
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC         Evidence={ECO:0000256|PIRSR:PIRSR604294-1};
CC       Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR604294-1};
CC   -!- SIMILARITY: Belongs to the carotenoid oxygenase family.
CC       {ECO:0000256|ARBA:ARBA00006787}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PWA50047.1}.
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DR   EMBL; PKPP01008682; PWA50047.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2U1LM23; -.
DR   STRING; 35608.A0A2U1LM23; -.
DR   Proteomes; UP000245207; Unassembled WGS sequence.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:InterPro.
DR   InterPro; IPR004294; Carotenoid_Oase.
DR   PANTHER; PTHR10543; BETA-CAROTENE DIOXYGENASE; 1.
DR   PANTHER; PTHR10543:SF24; CAROTENOID ISOMEROOXYGENASE; 1.
DR   Pfam; PF03055; RPE65; 1.
PE   3: Inferred from homology;
KW   Dioxygenase {ECO:0000256|ARBA:ARBA00022964};
KW   Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR604294-1};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR604294-1};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00022964};
KW   Reference proteome {ECO:0000313|Proteomes:UP000245207}.
FT   BINDING         242
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT   BINDING         292
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT   BINDING         359
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT   BINDING         549
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
SQ   SEQUENCE   556 AA;  61994 MW;  F2D82E4DCC3DDE53 CRC64;
     MASSLVSFLA TGNYISSSCR AISNHDIDSC TFGQASFPNK RKSRELSVVS VATELPLVVQ
     QQPWKGVEKE KKLVAWTSIQ QDRWEGELVV EGTIPRWLNG TYLRNGPGLW HLGDYDFRHL
     FDGYATMVRL HFDNGRLVMG HRQIESDAYK AARKNNKLCY REFSEVPKYD NFLAYIGDLA
     NLFSGASLTD NANTGVVQLG DGRVVCLTET IKGSIVIDPN NLDTLGKFEY SDTLGGLVHS
     AHPIVTESEF LTLLPDLLNP GYLVARMEPG SNERKVIGRV DCRGGPSPGW VHSFPVTEHY
     VIVPEMPLRY CAQNLLRAEP TPLYKFEWHP ESKAYMHVMC KASGKLVASV EVPLFVTFHF
     INAYEEKDED GRVTGVIADC CEHNANTTIL DKLRLKNLRS WSGEDVLPDA RVGRFKIPFN
     GSPKGELVDA LNPNEHGRGM DMCSINPAYL GKKYRYAYAC GAQRPCNFPN TLTKIDLKEK
     KAKNWHDEGA IPSEPFFVAR PGATNEDDGV VISMVSGQNG EGYALILDAS TFEEIARAKF
     PYGLPYGLHG CWVPKR
//