ID A0A2U1N056_ARTAN Unreviewed; 1133 AA.
AC A0A2U1N056;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=DNA polymerase {ECO:0000256|RuleBase:RU000442};
DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU000442};
GN ORFNames=CTI12_AA323620 {ECO:0000313|EMBL:PWA66889.1};
OS Artemisia annua (Sweet wormwood).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Asteroideae; Anthemideae;
OC Artemisiinae; Artemisia.
OX NCBI_TaxID=35608 {ECO:0000313|EMBL:PWA66889.1, ECO:0000313|Proteomes:UP000245207};
RN [1] {ECO:0000313|EMBL:PWA66889.1, ECO:0000313|Proteomes:UP000245207}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Huhao1 {ECO:0000313|Proteomes:UP000245207};
RC TISSUE=Leaf {ECO:0000313|EMBL:PWA66889.1};
RX PubMed=29703587; DOI=10.1016/j.molp.2018.03.015;
RA Shen Q., Zhang L., Liao Z., Wang S., Yan T., Shi P., Liu M., Fu X., Pan Q.,
RA Wang Y., Lv Z., Lu X., Zhang F., Jiang W., Ma Y., Chen M., Hao X., Li L.,
RA Tang Y., Lv G., Zhou Y., Sun X., Brodelius P.E., Rose J.K.C., Tang K.;
RT "The genome of Artemisia annua provides insight into the evolution of
RT Asteraceae family and artemisinin biosynthesis.";
RL Mol. Plant 11:776-788(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|RuleBase:RU000442};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family.
CC {ECO:0000256|ARBA:ARBA00005755, ECO:0000256|RuleBase:RU000442}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PWA66889.1}.
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DR EMBL; PKPP01003943; PWA66889.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2U1N056; -.
DR STRING; 35608.A0A2U1N056; -.
DR Proteomes; UP000245207; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:1902975; P:mitotic DNA replication initiation; IEA:InterPro.
DR CDD; cd05776; DNA_polB_alpha_exo; 1.
DR CDD; cd05532; POLBc_alpha; 1.
DR Gene3D; 3.30.70.2820; -; 1.
DR Gene3D; 1.10.3200.20; DNA Polymerase alpha, zinc finger; 1.
DR Gene3D; 1.10.132.60; DNA polymerase family B, C-terminal domain; 1.
DR Gene3D; 1.10.287.690; Helix hairpin bin; 1.
DR Gene3D; 3.90.1600.10; Palm domain of DNA polymerase; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR042087; DNA_pol_B_thumb.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR038256; Pol_alpha_znc_sf.
DR InterPro; IPR045846; POLBc_alpha.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR015088; Znf_DNA-dir_DNA_pol_B_alpha.
DR NCBIfam; TIGR00592; pol2; 1.
DR PANTHER; PTHR45861; DNA POLYMERASE ALPHA CATALYTIC SUBUNIT; 1.
DR PANTHER; PTHR45861:SF1; DNA POLYMERASE ALPHA CATALYTIC SUBUNIT; 1.
DR Pfam; PF00136; DNA_pol_B; 1.
DR Pfam; PF03104; DNA_pol_B_exo1; 1.
DR Pfam; PF08996; zf-DNA_Pol; 1.
DR PRINTS; PR00106; DNAPOLB.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE 3: Inferred from homology;
KW DNA replication {ECO:0000256|RuleBase:RU000442};
KW DNA-binding {ECO:0000256|RuleBase:RU000442};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU000442};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU000442};
KW Reference proteome {ECO:0000313|Proteomes:UP000245207};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000442};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT DOMAIN 52..369
FT /note="DNA-directed DNA polymerase family B exonuclease"
FT /evidence="ECO:0000259|Pfam:PF03104"
FT DOMAIN 434..890
FT /note="DNA-directed DNA polymerase family B
FT multifunctional"
FT /evidence="ECO:0000259|Pfam:PF00136"
FT DOMAIN 930..1130
FT /note="Zinc finger DNA-directed DNA polymerase family B
FT alpha"
FT /evidence="ECO:0000259|Pfam:PF08996"
FT REGION 470..491
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1133 AA; 127112 MW; 92215F279B41EE11 CRC64;
MQRCVYAIPS DSVFEDPVIA KLEQDVKDSK MLPTAFHTKL HEMASGLKTN LTKLLLDRNV
SNFSMKPVKR NYAFERSDIE RKEHYVLKIS YPFKDPPLPA DLKGETFCAL LGTHSSALEL
FLIKRKIKGP SWLSISKFTN CSTAQRVSWC KFEVTVDCSK DIKVATNPEN TREIPPVIVT
AINLKTVINQ KQNTNEVASA SVVCCHKAKI DTPMLASEWT RSGMLSHFTV VRKLEDGLFP
MGFSKEAADK NAKAGSNIIS TESSERALLN RLMIELHKLD SDILVGHNIS GFDLDVLLNR
VKACKVPSSM WSKIGRLKRS EMPRLNKGNT VFGSGASPGI MSCIAGRLLC DTFLCSRDLL
KEVSYSLTEL AKTQLNKNRK EIAPHDIPRL FQSSQTLMEL IETGETDAWL SMELMFHLCV
LPLTRQLTNI SGNLWGKTLQ GARAQRVEYL LLHAFHAKKF MVPDKFYSPK ESKTAKRKPD
GSEDKVTNDL GVNDEIENEP VKSKKSASYA GGLVLEPKKG LYDKYILLLD FNSLYPSIIQ
EYNICFTTVE RSDDSLISRL PSSKATGVLP ELLKNLVERR KSVKRSMKSA TGLKHQQFDI
QQQALKLTAN SMYGCLGFSN SRFYAKPLAE LITLQGREIL QQTVDLVQNN LNFEVIYGDT
DSIMIYTGLD DINKAKSIAG NVIREVNKKY KCLEIDLDGV YKRMLLLKKK KYAAVKEQTK
DGKIYKVIER KGLDMVRRDW SLLSKETGDF CLEQILSELS CEEVVESIHD KLRKVQEDMR
NGQIALEKYI ITKSLTKPPE AYPDARSQPH VEVALRLRKL GYTSGCSAGD TVPYIICCEQ
GKGSSTSAGI AQRARHPDEL KKDNENLMID IDYYLAQQIH PVVSRLCASI EGTSPSMLAN
CLGLDPSKFQ SKSSEVANND HSGSVLGVAD DEERYRGCEP LTLSCPGCSG TFDCPSIISY
IRTLAVEKST DVQDQSSNTS FWNKLCCPKC QVEVPSVSVA NQVKKRAELF ISTYYKGLMT
CDDETCDYTT RSLNLRVIGD SERGTVCPNY PRCNGRLVRQ YSEANVYKQL SYFCYLLDAN
RCIDKVEAKF KISVEKEVAR ITPLVELASL TVQKVRDRCG YGWVKLGDLF STS
//
