ID A0A2U1NIS9_ARTAN Unreviewed; 1222 AA.
AC A0A2U1NIS9;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=RNA helicase {ECO:0000256|ARBA:ARBA00012552};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
GN ORFNames=CTI12_AA261300 {ECO:0000313|EMBL:PWA73424.1};
OS Artemisia annua (Sweet wormwood).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Asteroideae; Anthemideae;
OC Artemisiinae; Artemisia.
OX NCBI_TaxID=35608 {ECO:0000313|EMBL:PWA73424.1, ECO:0000313|Proteomes:UP000245207};
RN [1] {ECO:0000313|EMBL:PWA73424.1, ECO:0000313|Proteomes:UP000245207}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Huhao1 {ECO:0000313|Proteomes:UP000245207};
RC TISSUE=Leaf {ECO:0000313|EMBL:PWA73424.1};
RX PubMed=29703587; DOI=10.1016/j.molp.2018.03.015;
RA Shen Q., Zhang L., Liao Z., Wang S., Yan T., Shi P., Liu M., Fu X., Pan Q.,
RA Wang Y., Lv Z., Lu X., Zhang F., Jiang W., Ma Y., Chen M., Hao X., Li L.,
RA Tang Y., Lv G., Zhou Y., Sun X., Brodelius P.E., Rose J.K.C., Tang K.;
RT "The genome of Artemisia annua provides insight into the evolution of
RT Asteraceae family and artemisinin biosynthesis.";
RL Mol. Plant 11:776-788(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PWA73424.1}.
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DR EMBL; PKPP01002740; PWA73424.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2U1NIS9; -.
DR STRING; 35608.A0A2U1NIS9; -.
DR Proteomes; UP000245207; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IEA:InterPro.
DR CDD; cd17971; DEXHc_DHX8; 1.
DR CDD; cd21691; GH2-like_DHX8; 1.
DR CDD; cd05684; S1_DHX8_helicase; 1.
DR CDD; cd18791; SF2_C_RHA; 1.
DR Gene3D; 1.20.120.1080; -; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR044762; DHX8/Prp22_DEXHc.
DR InterPro; IPR049588; DHX8_GH2-like.
DR InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR InterPro; IPR048333; HA2_WH.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049621; S1_DHX8_helicase.
DR InterPro; IPR003029; S1_domain.
DR PANTHER; PTHR18934; ATP-DEPENDENT RNA HELICASE; 1.
DR PANTHER; PTHR18934:SF85; ATP-DEPENDENT RNA HELICASE DHX8; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF21010; HA2_C; 1.
DR Pfam; PF04408; HA2_N; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR Pfam; PF00575; S1; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00316; S1; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS50126; S1; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000313|EMBL:PWA73424.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664};
KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000245207}.
FT DOMAIN 264..333
FT /note="S1 motif"
FT /evidence="ECO:0000259|PROSITE:PS50126"
FT DOMAIN 579..742
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 760..940
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 131..254
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 341..368
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 493..521
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1222 AA; 139340 MW; 6647E2D99706EC9B CRC64;
MKPASKPTKK KDTQSLKKLE YLSLVSKVCT ELESHLGFTD KVLAEFITDL GRKCLTHEEF
DQKLKQNGAD LPDYFVRTLL TIIHAILPPK VKKSKGVDSD KGALGIGDSR DHVRSLEKEI
EMEALEKARL RGENLDNGEN GENRREKREN RDRYDRKRDD RDNDRFERKR GDRDNERARD
RDNERYERRR DDIGRDRGEN DENEPRSRRE RDRDRYDKKR DDKGRDRGDK YERKRDDRDD
DRYERKGGDS RDGVNRRMDE LELYQVYRGR VTRIMEHGCF VEILDVKGKE GLVHVSQMAT
RRVANAKDVV KRDQEVFVKV ISVSTGKVSL SMRDVDQNTG KDLLPLKKSG EDGGGDGGRV
NVGSGVDNGN NKSRIGLSGI KLTDEDVGVS SRRPLKKMSS PERWEANQLI ASGVLSVKDY
PMYDEETNDG MVYQEEGGDE ELEVELNEDE PAFLKGQSHY SMDMSPVKIF KNPEGSLSRA
AALQSALIKE RREVRDQQQR TMLDSIPKDL NRPWEDPMPE NGERHLAQEL RGVGLSAYDM
PEWKKDSFSK ALTFGQRSKL SLQEQRQSLP IYKLKKELVQ AVNDNQVLVV IGETGSGKTT
QVTQYLAEAG YTTRGKIGCT QPRRVAAMSV AKRVAEEFGC RLGEEVGYSI RFEDCTGPET
VIKYMTDGML LREILVDENL SQYSVIMLDE AHERTMSTDV LFGLLKGLIK RRPDLRLIVT
SATLDAEKFS GYFFSCNIFT IPGRTFPVEI LYTKQPESDY VDAALITVMQ IHLTEPEGDI
LMFLTGQEEI DHACQCLFER MKGLGKNVPE LIILPVYSAL PSEMQSRIFE PAPPGKRKVV
VATNIAEASL TIDGIFYVID PGFAKQNVYN PKQGLDSLVI TPISQASAKQ RAGRAGRTGP
GKCYRLYTEN AFNNEMSPTS IPEIQRINLA LVTLNLKAMG INDLLAFDFM DPPSPQALIA
ALEQLYTLGA LDEEGLLTKL GRKMAEFPLD PPLSKMLLAS VDLGCSDEIL TIIAMITTGN
IFYRPREKQA QADQKKAKFF QPEGDHLTLL AVYEAWKAKN FSGPWCFENF VQSRSLRRAQ
DVRKQLLSIM DKYKLDIVSA GKNFTKIRKA ITAGFFFHAA RKDPQEGYRT IVENQPVYVH
PSSALFQRQP DWVIYHELVM TTKEYMREVT VVDPKWLVEL APRFFKVSDP TKMSKRKRQE
RIEPLYDRYN EPNSWRLSKR RA
//
