ID A0A2U1QLK3_ARTAN Unreviewed; 1759 AA.
AC A0A2U1QLK3;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU363044};
DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU363044};
GN ORFNames=CTI12_AA004850 {ECO:0000313|EMBL:PWA98889.1};
OS Artemisia annua (Sweet wormwood).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Asteroideae; Anthemideae;
OC Artemisiinae; Artemisia.
OX NCBI_TaxID=35608 {ECO:0000313|EMBL:PWA98889.1, ECO:0000313|Proteomes:UP000245207};
RN [1] {ECO:0000313|EMBL:PWA98889.1, ECO:0000313|Proteomes:UP000245207}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Huhao1 {ECO:0000313|Proteomes:UP000245207};
RC TISSUE=Leaf {ECO:0000313|EMBL:PWA98889.1};
RX PubMed=29703587; DOI=10.1016/j.molp.2018.03.015;
RA Shen Q., Zhang L., Liao Z., Wang S., Yan T., Shi P., Liu M., Fu X., Pan Q.,
RA Wang Y., Lv Z., Lu X., Zhang F., Jiang W., Ma Y., Chen M., Hao X., Li L.,
RA Tang Y., Lv G., Zhou Y., Sun X., Brodelius P.E., Rose J.K.C., Tang K.;
RT "The genome of Artemisia annua provides insight into the evolution of
RT Asteraceae family and artemisinin biosynthesis.";
RL Mol. Plant 11:776-788(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- SIMILARITY: Belongs to the helicase family.
CC {ECO:0000256|RuleBase:RU363044}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PWA98889.1}.
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DR EMBL; PKPP01000044; PWA98889.1; -; Genomic_DNA.
DR STRING; 35608.A0A2U1QLK3; -.
DR Proteomes; UP000245207; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR CDD; cd18809; SF1_C_RecD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR010285; DNA_helicase_pif1-like.
DR InterPro; IPR025476; Helitron_helicase-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049163; Pif1-like_2B_dom.
DR PANTHER; PTHR45786; DNA BINDING PROTEIN-LIKE; 1.
DR PANTHER; PTHR45786:SF7; OS12G0539700 PROTEIN; 1.
DR Pfam; PF14214; Helitron_like_N; 1.
DR Pfam; PF05970; PIF1; 1.
DR Pfam; PF21530; Pif1_2B_dom; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU363044};
KW DNA damage {ECO:0000256|RuleBase:RU363044};
KW DNA recombination {ECO:0000256|RuleBase:RU363044};
KW DNA repair {ECO:0000256|RuleBase:RU363044};
KW Helicase {ECO:0000256|RuleBase:RU363044, ECO:0000313|EMBL:PWA98889.1};
KW Hydrolase {ECO:0000256|RuleBase:RU363044};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU363044};
KW Reference proteome {ECO:0000313|Proteomes:UP000245207}.
FT DOMAIN 599..777
FT /note="Helitron helicase-like"
FT /evidence="ECO:0000259|Pfam:PF14214"
FT DOMAIN 1568..1614
FT /note="DNA helicase Pif1-like 2B"
FT /evidence="ECO:0000259|Pfam:PF21530"
FT REGION 173..201
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 260..310
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 176..201
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 260..302
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1759 AA; 198810 MW; 2798116A4174EA24 CRC64;
MKTKSKASKK DDALGFVTDP LPHHLGVISL EEPNDQNLWR LSSKDARTQS IVNAGHVVES
SASKRKVVSP AFSPLRLDEK RLKRGDDGCA SSNDCVVTHN SSGSRSGVQF WSTQGWTTGS
FSHDQRPVVD QVVSHEPGRG GQLGIVDVST SVVAPNEGIT LAGNSADRRL KRKAGSLQRQ
PSNNTINKAR RTITDHPSLP FETQLGETVP VQRNTYFRGS SNMPLILDFE SGHARPAGHS
FSSPRGHQRC ASPLLPSRMT TAATASVQSD SGSPTAEAPN SVPENDVGNQ RSRPQFHETQ
RRGGSSGPPA EYKAFGRCNR VCRNCRAIFW DEEKLSTSTG STGPLYHRCC LQGRVKLFVP
REYPPLIQQL FSDSHFLENI RAYNQMFAMT SLGATIDESV NTGTGPYVFK ISGQIYHRIG
RLCPDRDKPP RFLQLYIYDT VNEVQHRLGH FTSNGHPVLR REIVEQLIRF LDHNNALVQL
FRMARDKLLQ NDVPEFKVRL FNVGSRSQYE LPTADAIGAI VFDDTAETES DFDIILETHS
GEPQRINKLH ACYMSAQFPL LFVYGEQGYH TDLKFLNVDG VYTRRGKRLS MKAFYLYQLH
DRLGYYNLLS RGGKLFQQYV VTAYCSIEQS RMDYIRTHQS DIRGDYLAGL YDAITRGDTD
GSDIGSRIIL PSSFTGGPRY MYSHYLDALA ICRKHGNPSF FITFTCNTKW PEIEDYMEAY
PRLAAADRPD VVDRVFECKI HSLIKYIRDR QPFGVLTAVL YTVEFQKRGL PHCHILIWIN
QRPETRRDSD IDRYISAELP DQVIDPEAFR VVSEFMVHGP CGEAFPNAPC MINRSTCKKF
FPKEYCHATF VDNAGYVHYR RRDIAINATR HNVKLDNAYV VPYNRELCMA FYAHINVEYC
GWTMLIKYLF KYISKGTDRI MARITRAVGE QPHPPSVASS SNGQPYVQVD EIQNFVDARY
IGPHEACWRI FGFDIHNRDP AVQILAVHLE NMQTVTFRRR HRLQAIVDNP GSTKTTLTEW
LAFNKSFEMG RHLTYLEFPS EFVWSKRDKS WSPRCNLNRP AIGRLTYVHP SAGDLFYQRI
LLCHQRGCTS FPSIRTVDGY THSTNRSACE AMGLLQDDQE WITAMQEAAA TATASQLRAL
FSEILVFTDI CSPLKLWEMF WKHMSDDIPL ILSQSLGIRL LCVNEPELRG GVVYELQVLL
ALHSKYLKDF GLPSPPEGLL NMFRNRAVME ERNYNREELA AESRSLIPQL NVEQKDVFDT
IMRAVTQQKQ ELLFVYGHGG TGKTFLWKTV IATLRSQGKI VLAVASSGIA SLLLPSGRTA
HSRFKIPLQL ADESVCSVKK GTQLGELLKQ TDLVIWDEAP MSERRCFEAL DRTFRDILDE
PRLVFGGKPV ALGGDFRQTL PVKKKATRDQ IIAVSIAESY LWRHFKIFFL EENMRLRRPS
MTMAEQQEIA LFSEWLLNIG DANIGSQDVD DPQNTSWVRI PDKHCFPPTA DGILQLIEFI
YDNSSLQQPT ASSFQEKAIV CPKNETADLI NAQILDKVSG PSQFYLSVDE AVPVGNDGGA
SELLYPIEYL NSMKFPGIPP HRLEMKIGSP VMLLRNLNLT GGLCNGTRMI ISQLLSKVIE
AKIISGVRIG QKVFLPRIIL SVKEENMPFT FKRKQFPIKL CYAMTINKSQ GQSLKKIGVY
LPEPIFGHGQ LYVALSRATS PDGLKMVVAH QEGQPPNTTK NIVYKDFLRK LRRALLKNVT
EPVIAGLFKI PEKETDELA
//
