ID   A0A2U2PK85_9SPHI        Unreviewed;       557 AA.
AC   A0A2U2PK85;
DT   18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT   18-JUL-2018, sequence version 1.
DT   24-JAN-2024, entry version 19.
DE   SubName: Full=Peptidase M1 {ECO:0000313|EMBL:PWG81827.1};
GN   ORFNames=DDR33_05565 {ECO:0000313|EMBL:PWG81827.1};
OS   Pararcticibacter amylolyticus.
OC   Bacteria; Bacteroidota; Sphingobacteriia; Sphingobacteriales;
OC   Sphingobacteriaceae; Pararcticibacter.
OX   NCBI_TaxID=2173175 {ECO:0000313|EMBL:PWG81827.1, ECO:0000313|Proteomes:UP000245647};
RN   [1] {ECO:0000313|EMBL:PWG81827.1, ECO:0000313|Proteomes:UP000245647}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FJ4-8 {ECO:0000313|EMBL:PWG81827.1,
RC   ECO:0000313|Proteomes:UP000245647};
RA   Cai Y.;
RT   "Pedobacter chongqingensis sp. nov., isolated from a rottenly hemp rope.";
RL   Submitted (APR-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|PIRSR:PIRSR634015-3};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000256|PIRSR:PIRSR634015-3};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PWG81827.1}.
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DR   EMBL; QEAS01000003; PWG81827.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2U2PK85; -.
DR   OrthoDB; 100605at2; -.
DR   Proteomes; UP000245647; Unassembled WGS sequence.
DR   GO; GO:0008237; F:metallopeptidase activity; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd09603; M1_APN_like; 1.
DR   Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR   Gene3D; 2.60.40.1730; tricorn interacting facor f3 domain; 1.
DR   InterPro; IPR042097; Aminopeptidase_N-like_N_sf.
DR   InterPro; IPR034015; M1_LTA4H.
DR   InterPro; IPR014782; Peptidase_M1_dom.
DR   InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR   PANTHER; PTHR45726; LEUKOTRIENE A-4 HYDROLASE; 1.
DR   PANTHER; PTHR45726:SF3; LEUKOTRIENE A-4 HYDROLASE; 1.
DR   Pfam; PF01433; Peptidase_M1; 1.
DR   SUPFAM; SSF63737; Leukotriene A4 hydrolase N-terminal domain; 1.
DR   SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE   4: Predicted;
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR634015-3};
KW   Reference proteome {ECO:0000313|Proteomes:UP000245647};
KW   Signal {ECO:0000256|SAM:SignalP}; Zinc {ECO:0000256|PIRSR:PIRSR634015-3}.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           27..557
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5015446772"
FT   DOMAIN          339..479
FT                   /note="Peptidase M1 membrane alanine aminopeptidase"
FT                   /evidence="ECO:0000259|Pfam:PF01433"
FT   ACT_SITE        345
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-1"
FT   ACT_SITE        419
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-1"
FT   BINDING         344
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-3"
FT   BINDING         348
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-3"
FT   BINDING         367
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-3"
SQ   SEQUENCE   557 AA;  63391 MW;  F62DADA70C39AF87 CRC64;
     MSHNISNLIK CCVLAAAFAL PGGASAQLMA DKETFTRADS LRGNLTDLRT CYDIRYYHLD
     VKLDIGKKFI SGSNVFEFTA TRDFNRLQFD LFDNLKVEKI EYNGTELQFT REFNAVFVSF
     PNTVKQGQRD RFKVFYSGKP VEAKNAPWDG GVMFSKDDSG KPWVATACQG VGASIWWPNK
     DHQSDEVDSM LISVTVPEGL QDVSNGRLRK VSPAGNGYTR YDWFVANPIN NYCVSLNIGD
     YDHWGEAFDG EKGKLDLDFW VLKANRKKAE KQFAANVKPM LKAFEYWFGP YPFYEDGYKL
     VETPHLGMEH QSAVAYGNKF QNGYMGRDLS GTGWGLKWDF IVVHESGHEW FGNNITSKDL
     ADMWIHESFT NYSESLFIES LYGKKAGQEY VHGTRGAILN DKPVQGIYNV NKEGSGDMYY
     KGGNLLNMIR TIINDDTKWR HILRGLNKTF YHRTVDATDI ISYINGQSGI DLSKVFGQYL
     KFKNIPTLEV KFEKGKTFCR WIADVDDFDM PVRLRVKGGE YQFVKPSTSF AEIALPGATK
     ENFEVDSFNY YIGLLLE
//