ID A0A2U2RLS5_9MICO Unreviewed; 446 AA.
AC A0A2U2RLS5;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE SubName: Full=Zinc metalloprotease {ECO:0000313|EMBL:PWH06734.1};
GN ORFNames=DEO23_07360 {ECO:0000313|EMBL:PWH06734.1};
OS Brachybacterium endophyticum.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Dermabacteraceae;
OC Brachybacterium.
OX NCBI_TaxID=2182385 {ECO:0000313|EMBL:PWH06734.1, ECO:0000313|Proteomes:UP000245590};
RN [1] {ECO:0000313|EMBL:PWH06734.1, ECO:0000313|Proteomes:UP000245590}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M1HQ-2 {ECO:0000313|EMBL:PWH06734.1,
RC ECO:0000313|Proteomes:UP000245590};
RA Tuo L.;
RT "Brachybacterium sp. M1HQ-2T, whole genome shotgun sequence.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the peptidase M50B family.
CC {ECO:0000256|ARBA:ARBA00007931}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PWH06734.1}.
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DR EMBL; QFKX01000002; PWH06734.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2U2RLS5; -.
DR OrthoDB; 9782003at2; -.
DR Proteomes; UP000245590; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd00989; PDZ_metalloprotease; 1.
DR CDD; cd06163; S2P-M50_PDZ_RseP-like; 1.
DR Gene3D; 2.30.42.10; -; 1.
DR InterPro; IPR041489; PDZ_6.
DR InterPro; IPR036034; PDZ_sf.
DR InterPro; IPR004387; Pept_M50_Zn.
DR InterPro; IPR008915; Peptidase_M50.
DR PANTHER; PTHR42837:SF2; MEMBRANE METALLOPROTEASE ARASP2, CHLOROPLASTIC-RELATED; 1.
DR PANTHER; PTHR42837; REGULATOR OF SIGMA-E PROTEASE RSEP; 1.
DR Pfam; PF17820; PDZ_6; 1.
DR Pfam; PF02163; Peptidase_M50; 1.
DR SUPFAM; SSF50156; PDZ domain-like; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049,
KW ECO:0000313|EMBL:PWH06734.1};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000313|EMBL:PWH06734.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000245590};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT TRANSMEM 6..23
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 137..156
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 368..394
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 415..436
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 14..396
FT /note="Peptidase M50"
FT /evidence="ECO:0000259|Pfam:PF02163"
FT DOMAIN 186..232
FT /note="PDZ"
FT /evidence="ECO:0000259|Pfam:PF17820"
SQ SEQUENCE 446 AA; 47041 MW; 0F7E735B9395D0FF CRC64;
MTALLYALGV VVVALGIAVS IALHELGHLV PAKAFGVRVT QYMIGFGPTI LSRRRGETEY
GLKAFPLGGY IRMIGMYPPH KGDAPGTIRE DSTGLVQQMS RDAREWEASQ YDPSEAHRTF
SALSVPRKLV VMLGGPTMNL LISAVLLAVL VCGIGLPSTS TTVESVSRCV LPASAPAGTS
CEGRQEAPAL AAGIRPGDRI LSVDGRGIDE WSDVTTAVRA AGGSRIDVVV ERDGARKTLH
ATPILDQRPK IAADGSAQKN PDGSIATEKV GFLGIGPGQE LQRSSPLEVP GMVWQTFEGT
ARIVITLPMR LVDVAQAAFG DGERDPDGPV GVVGVSRLAG EVASADQPEF DLTAKVSTLI
SMLASLNMAL FVFNLVPLMP LDGGHVAGAL VEGVRRFVAR LRGRPDPGPV DMSRLLPLTN
VVALAFLAMT VMLLYADIVD PITLFP
//
