ID A0A2U2RMP9_9MICO Unreviewed; 1108 AA.
AC A0A2U2RMP9;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=DEO23_07960 {ECO:0000313|EMBL:PWH07065.1};
OS Brachybacterium endophyticum.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Dermabacteraceae;
OC Brachybacterium.
OX NCBI_TaxID=2182385 {ECO:0000313|EMBL:PWH07065.1, ECO:0000313|Proteomes:UP000245590};
RN [1] {ECO:0000313|EMBL:PWH07065.1, ECO:0000313|Proteomes:UP000245590}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M1HQ-2 {ECO:0000313|EMBL:PWH07065.1,
RC ECO:0000313|Proteomes:UP000245590};
RA Tuo L.;
RT "Brachybacterium sp. M1HQ-2T, whole genome shotgun sequence.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PWH07065.1}.
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DR EMBL; QFKX01000002; PWH07065.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2U2RMP9; -.
DR OrthoDB; 4812256at2; -.
DR Proteomes; UP000245590; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 4.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF55; DNA HELICASE; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000245590}.
FT DOMAIN 4..345
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 346..670
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 405..428
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 414..428
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 25..32
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1108 AA; 120258 MW; 985BED1F0BA24FA3 CRC64;
MGRPAPTPEQ TAVIEAPLAP MLVVAGAGSG KTETMASRVV WLIANGHVEP RQVLGLTFTR
KAAHELAERI AGRLSTLASV LRAEGLPLPR GLARAGQDLL GQDVQVHTYN GFALDLVREH
ALRVGLDPEF TMMSPSAAWQ LAHELVEAAD DTLALDASPA SLTAALVSLT SSLADHLVSP
AELAAELRAI REHLAQIPLQ VSGRRRTTPK DVAAVVSACE QRLELIGLIE EFARVRTERS
ALEFSDQVHL AARIAREVPG AGRLARELHP VVLLDEFQDT SVAQLRMLAD LFGPGHAACA
VGDPQQAIYG WRGASAASLD GFARSFASET QEVLQRTLST SWRNDRAVLD VANRVAAPLR
SASTAITIPE LQVRPGAGEG TVHVHEAADE QTEARAIAAW IRRRRGPEVR GDDSSSSGPD
TTSTPLPSAA VLVRARRQIP PIVEALEEAG LPVQVMGLGG LLDRPEVADV RALLECVHDP
SRGDWLMRLL AGPRFRLGSR DIAVLGRWRE RLGARRRRGV GEAGTVDDAD EMSLIDAVDD
LPPADWADSE GRSLSAAARE RLLDLQGMLR HLRRRLALPL PDLVHEAVRA LDLDLTLLAD
ADSSPALGGL PALREHAAAF ERTAQRPGLG PFLALLEISE DQEAGLSMGA GQADPDPDAV
TVVTMHSAKG LEWDLVAVAG LSEGSVPSYD LRRARTTEDG TVRVADSGWL GKLADASIPS
SLRGDADILP QLDWAAADTQ VDAESLIQQF RFDQGEESLR EDRRLMYVAV TRAREDLLLS
SCAWRTGAAK PRPRSRYLRE VLPQVPAPFQ VLEDVPEKNP LDALLLEAEW PPPPGEAEQR
RHEAERLLAA ASEGDLPEVP EDAELAEQTR RVLADLAERR EQLTVRSPAR LSASQIVARA
QDPQAAARAM LRPLPRRPSR SARRGTAFHA WIEQRLEGGA LLDLEDIAEF ADLEEQEQDR
TQDRADADAG ADTDAAALAR MRESFESSPY ASQVPIAVEE PVTTRIGPLA IRGVIDAVFP
DPEHPDGVVI LDWKTGSPPT GARRRARALQ LSVYRLAWHE RTGMPLSRIR TVFYYVADGV
SHEVTHHPSR SSMERMLLAA DEETSPER
//
